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פותח על ידי קלירמאש פתרונות בע"מ -
ETHQV6.3 is involved in melon climacteric fruit ripening and is encoded by a NAC domain transcription factor
Year:
2017
Source of publication :
Plant Journal
Authors :
קניגסוולד, מרב
;
.
Volume :
91
Co-Authors:
Ríos, P., IRTA, Centre for Research in Agricultural Genomics CSIC-IRTA-UAB-UB, Barcelona, Spain, Syngenta España S.A, El Ejido, Spain
Argyris, J., IRTA, Centre for Research in Agricultural Genomics CSIC-IRTA-UAB-UB, Barcelona, Spain
Vegas, J., IRTA, Centre for Research in Agricultural Genomics CSIC-IRTA-UAB-UB, Barcelona, Spain, Sesvanderhave N.V, Tienen, Belgium
Leida, C., Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universitat Politècnica de València (UPV)-Consejo Superior de Investigaciones Científicas (CSIC), Valencia, Spain
Kenigswald, M., Department of Vegetable Research, Agricultural Research Organization (ARO), Newe Ya'ar Research Center, Ramat Yishay, Israel, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Jerusalem, Israel
Tzuri, G., Department of Vegetable Research, Agricultural Research Organization (ARO), Newe Ya'ar Research Center, Ramat Yishay, Israel
Troadec, C., Institute of Plant Sciences Paris-Saclay (IPS2), INRA, CNRS, University of Paris-Sud, University of Evry, University Paris-Diderot, Sorbone Paris-Cité, University of Paris-Saclay, Orsay, France
Bendahmane, A., Institute of Plant Sciences Paris-Saclay (IPS2), INRA, CNRS, University of Paris-Sud, University of Evry, University Paris-Diderot, Sorbone Paris-Cité, University of Paris-Saclay, Orsay, France
Katzir, N., Department of Vegetable Research, Agricultural Research Organization (ARO), Newe Ya'ar Research Center, Ramat Yishay, Israel
Picó, B., Institute for the Conservation and Breeding of the Agricultural Biodiversity, COMAV-UPV, Universitat Politècnica de València, Valencia, Spain
Monforte, A.J., Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universitat Politècnica de València (UPV)-Consejo Superior de Investigaciones Científicas (CSIC), Valencia, Spain
Garcia-Mas, J., IRTA, Centre for Research in Agricultural Genomics CSIC-IRTA-UAB-UB, Barcelona, Spain
Facilitators :
From page:
671
To page:
683
(
Total pages:
13
)
Abstract:
Fruit ripening is divided into climacteric and non-climacteric types depending on the presence or absence of a transient rise in respiration rate and the production of autocatalytic ethylene. Melon is ideal for the study of fruit ripening, as both climacteric and non-climacteric varieties exist. Two introgressions of the non-climacteric accession PI 161375, encompassed in the QTLs ETHQB3.5 and ETHQV6.3, into the non-climacteric ‘Piel de Sapo’ background are able to induce climacteric ripening independently. We report that the gene underlying ETHQV6.3 is MELO3C016540 (CmNAC-NOR), encoding a NAC (NAM, ATAF1,2, CUC2) transcription factor that is closely related to the tomato NOR (non-ripening) gene. CmNAC-NOR was functionally validated through the identification of two TILLING lines carrying non-synonymous mutations in the conserved NAC domain region. In an otherwise highly climacteric genetic background, both mutations provoked a significant delay in the onset of fruit ripening and in the biosynthesis of ethylene. The PI 161375 allele of ETHQV6.3 is similar to that of climacteric lines of the cantalupensis type and, when introgressed into the non-climacteric ‘Piel de Sapo’, partially restores its climacteric ripening capacity. CmNAC-NOR is expressed in fruit flesh of both climacteric and non-climacteric lines, suggesting that the causal mutation may not be acting at the transcriptional level. The use of a comparative genetic approach in a species with both climacteric and non-climacteric ripening is a powerful strategy to dissect the complex mechanisms regulating the onset of fruit ripening. © 2017 The Authors The Plant Journal © 2017 John Wiley & Sons Ltd
Note:
Related Files :
Biochemistry
Cucumis melo
ETHQV6.3
ethylene
Fruits
Genes
introgression lines
TILLING mutant
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/tpj.13596
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22457
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:52
You may also be interested in
Scientific Publication
ETHQV6.3 is involved in melon climacteric fruit ripening and is encoded by a NAC domain transcription factor
91
Ríos, P., IRTA, Centre for Research in Agricultural Genomics CSIC-IRTA-UAB-UB, Barcelona, Spain, Syngenta España S.A, El Ejido, Spain
Argyris, J., IRTA, Centre for Research in Agricultural Genomics CSIC-IRTA-UAB-UB, Barcelona, Spain
Vegas, J., IRTA, Centre for Research in Agricultural Genomics CSIC-IRTA-UAB-UB, Barcelona, Spain, Sesvanderhave N.V, Tienen, Belgium
Leida, C., Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universitat Politècnica de València (UPV)-Consejo Superior de Investigaciones Científicas (CSIC), Valencia, Spain
Kenigswald, M., Department of Vegetable Research, Agricultural Research Organization (ARO), Newe Ya'ar Research Center, Ramat Yishay, Israel, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Jerusalem, Israel
Tzuri, G., Department of Vegetable Research, Agricultural Research Organization (ARO), Newe Ya'ar Research Center, Ramat Yishay, Israel
Troadec, C., Institute of Plant Sciences Paris-Saclay (IPS2), INRA, CNRS, University of Paris-Sud, University of Evry, University Paris-Diderot, Sorbone Paris-Cité, University of Paris-Saclay, Orsay, France
Bendahmane, A., Institute of Plant Sciences Paris-Saclay (IPS2), INRA, CNRS, University of Paris-Sud, University of Evry, University Paris-Diderot, Sorbone Paris-Cité, University of Paris-Saclay, Orsay, France
Katzir, N., Department of Vegetable Research, Agricultural Research Organization (ARO), Newe Ya'ar Research Center, Ramat Yishay, Israel
Picó, B., Institute for the Conservation and Breeding of the Agricultural Biodiversity, COMAV-UPV, Universitat Politècnica de València, Valencia, Spain
Monforte, A.J., Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universitat Politècnica de València (UPV)-Consejo Superior de Investigaciones Científicas (CSIC), Valencia, Spain
Garcia-Mas, J., IRTA, Centre for Research in Agricultural Genomics CSIC-IRTA-UAB-UB, Barcelona, Spain
ETHQV6.3 is involved in melon climacteric fruit ripening and is encoded by a NAC domain transcription factor
Fruit ripening is divided into climacteric and non-climacteric types depending on the presence or absence of a transient rise in respiration rate and the production of autocatalytic ethylene. Melon is ideal for the study of fruit ripening, as both climacteric and non-climacteric varieties exist. Two introgressions of the non-climacteric accession PI 161375, encompassed in the QTLs ETHQB3.5 and ETHQV6.3, into the non-climacteric ‘Piel de Sapo’ background are able to induce climacteric ripening independently. We report that the gene underlying ETHQV6.3 is MELO3C016540 (CmNAC-NOR), encoding a NAC (NAM, ATAF1,2, CUC2) transcription factor that is closely related to the tomato NOR (non-ripening) gene. CmNAC-NOR was functionally validated through the identification of two TILLING lines carrying non-synonymous mutations in the conserved NAC domain region. In an otherwise highly climacteric genetic background, both mutations provoked a significant delay in the onset of fruit ripening and in the biosynthesis of ethylene. The PI 161375 allele of ETHQV6.3 is similar to that of climacteric lines of the cantalupensis type and, when introgressed into the non-climacteric ‘Piel de Sapo’, partially restores its climacteric ripening capacity. CmNAC-NOR is expressed in fruit flesh of both climacteric and non-climacteric lines, suggesting that the causal mutation may not be acting at the transcriptional level. The use of a comparative genetic approach in a species with both climacteric and non-climacteric ripening is a powerful strategy to dissect the complex mechanisms regulating the onset of fruit ripening. © 2017 The Authors The Plant Journal © 2017 John Wiley & Sons Ltd
Scientific Publication
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