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A bulk segregant transcriptome analysis reveals metabolic and cellular processes associated with Orange allelic variation and fruit β-carotene accumulation in melon fruit
Year:
2015
Source of publication :
BMC Plant Biology
Authors :
אהלי, שחר
;
.
בורגר, יוסף
;
.
חיות, נועם
;
.
יסלזון, ילנה
;
.
לוינסון, אפרים
;
.
מאיר, איילה
;
.
פורטנוי, ויטלי
;
.
קציר, נורית
;
.
שפר, ארתור
;
.
תדמור, יעקב
;
.
Volume :
15
Co-Authors:
Chayut, N., Newe Ya'ar Research Center, Plant Science Institute, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, Israel, Technion - Israel Institute of Technology, Faculty of Biology, Haifa, Israel
Yuan, H., Cornell University, Plant Breeding and Genetics Section, School of Integrative Plant Science, Ithaca, NY, United States
Ohali, S., Newe Ya'ar Research Center, Plant Science Institute, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Meir, A., Newe Ya'ar Research Center, Plant Science Institute, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Yeselson, Y., The Volcani Center, Plant Science Institute, Agricultural Research Organization, P.O.B. 6, Bet-Dagan, Israel
Portnoy, V., Newe Ya'ar Research Center, Plant Science Institute, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Zheng, Y., Cornell University, Boyce Thompson Institute for Plant Research, Ithaca, NY, United States
Fei, Z., Cornell University, Boyce Thompson Institute for Plant Research, Ithaca, NY, United States
Lewinsohn, E., Newe Ya'ar Research Center, Plant Science Institute, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Katzir, N., Newe Ya'ar Research Center, Plant Science Institute, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Schaffer, A.A., The Volcani Center, Plant Science Institute, Agricultural Research Organization, P.O.B. 6, Bet-Dagan, Israel
Gepstein, S., Technion - Israel Institute of Technology, Faculty of Biology, Haifa, Israel
Burger, J., Newe Ya'ar Research Center, Plant Science Institute, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Li, L., Cornell University, Plant Breeding and Genetics Section, School of Integrative Plant Science, Ithaca, NY, United States, Cornell University, US Department of Agriculture-Agricultural Research Service, Robert W Holly Center for Agriculture and Health, Ithaca, NY, United States
Tadmor, Y., Newe Ya'ar Research Center, Plant Science Institute, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Facilitators :
From page:
0
To page:
0
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Total pages:
1
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Abstract:
Background: Melon fruit flesh color is primarily controlled by the "golden" single nucleotide polymorhism of the "Orange" gene, CmOr, which dominantly triggers the accumulation of the pro-vitamin A molecule, β-carotene, in the fruit mesocarp. The mechanism by which CmOr operates is not fully understood. To identify cellular and metabolic processes associated with CmOr allelic variation, we compared the transcriptome of bulks of developing fruit of homozygous orange and green fruited F3 families derived from a cross between orange and green fruited parental lines. Results: Pooling together F3 families that share same fruit flesh color and thus the same CmOr allelic variation, normalized traits unrelated to CmOr allelic variation. RNA sequencing analysis of these bulks enabled the identification of differentially expressed genes. These genes were clustered into functional groups. The relatively enriched functional groups were those involved in photosynthesis, RNA and protein regulation, and response to stress. Conclusions: The differentially expressed genes and the enriched processes identified here by bulk segregant RNA sequencing analysis are likely part of the regulatory network of CmOr. Our study demonstrates the resolution power of bulk segregant RNA sequencing in identifying genes related to commercially important traits and provides a useful tool for better understanding the mode of action of CmOr gene in the mediation of carotenoid accumulation. © 2015 Chayut et al.
Note:
Related Files :
Beta-carotene
carotenoids
Cucumis melo
Genetics
metabolism
molecular genetics
Molecular Sequence Data
Muskmelon
עוד תגיות
תוכן קשור
More details
DOI :
10.1186/s12870-015-0661-8
Article number:
274
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22463
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:52
Scientific Publication
A bulk segregant transcriptome analysis reveals metabolic and cellular processes associated with Orange allelic variation and fruit β-carotene accumulation in melon fruit
15
Chayut, N., Newe Ya'ar Research Center, Plant Science Institute, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, Israel, Technion - Israel Institute of Technology, Faculty of Biology, Haifa, Israel
Yuan, H., Cornell University, Plant Breeding and Genetics Section, School of Integrative Plant Science, Ithaca, NY, United States
Ohali, S., Newe Ya'ar Research Center, Plant Science Institute, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Meir, A., Newe Ya'ar Research Center, Plant Science Institute, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Yeselson, Y., The Volcani Center, Plant Science Institute, Agricultural Research Organization, P.O.B. 6, Bet-Dagan, Israel
Portnoy, V., Newe Ya'ar Research Center, Plant Science Institute, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Zheng, Y., Cornell University, Boyce Thompson Institute for Plant Research, Ithaca, NY, United States
Fei, Z., Cornell University, Boyce Thompson Institute for Plant Research, Ithaca, NY, United States
Lewinsohn, E., Newe Ya'ar Research Center, Plant Science Institute, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Katzir, N., Newe Ya'ar Research Center, Plant Science Institute, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Schaffer, A.A., The Volcani Center, Plant Science Institute, Agricultural Research Organization, P.O.B. 6, Bet-Dagan, Israel
Gepstein, S., Technion - Israel Institute of Technology, Faculty of Biology, Haifa, Israel
Burger, J., Newe Ya'ar Research Center, Plant Science Institute, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, Israel
Li, L., Cornell University, Plant Breeding and Genetics Section, School of Integrative Plant Science, Ithaca, NY, United States, Cornell University, US Department of Agriculture-Agricultural Research Service, Robert W Holly Center for Agriculture and Health, Ithaca, NY, United States
Tadmor, Y., Newe Ya'ar Research Center, Plant Science Institute, Agricultural Research Organization, P.O. Box 1021, Ramat Yishay, Israel
A bulk segregant transcriptome analysis reveals metabolic and cellular processes associated with Orange allelic variation and fruit β-carotene accumulation in melon fruit
Background: Melon fruit flesh color is primarily controlled by the "golden" single nucleotide polymorhism of the "Orange" gene, CmOr, which dominantly triggers the accumulation of the pro-vitamin A molecule, β-carotene, in the fruit mesocarp. The mechanism by which CmOr operates is not fully understood. To identify cellular and metabolic processes associated with CmOr allelic variation, we compared the transcriptome of bulks of developing fruit of homozygous orange and green fruited F3 families derived from a cross between orange and green fruited parental lines. Results: Pooling together F3 families that share same fruit flesh color and thus the same CmOr allelic variation, normalized traits unrelated to CmOr allelic variation. RNA sequencing analysis of these bulks enabled the identification of differentially expressed genes. These genes were clustered into functional groups. The relatively enriched functional groups were those involved in photosynthesis, RNA and protein regulation, and response to stress. Conclusions: The differentially expressed genes and the enriched processes identified here by bulk segregant RNA sequencing analysis are likely part of the regulatory network of CmOr. Our study demonstrates the resolution power of bulk segregant RNA sequencing in identifying genes related to commercially important traits and provides a useful tool for better understanding the mode of action of CmOr gene in the mediation of carotenoid accumulation. © 2015 Chayut et al.
Scientific Publication
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