חיפוש מתקדם
Plant, Cell and Environment
Heifetz, P.B., Devmtl., Cell and Molec. Biol. Group, Departments of Botany and Zoology, Duke University, Durham, NC 27708, United States, Novartis Crop Protection Inc., Biotechnology and Genomics Center, 3054 Cornwallis Road, Research Triangle Park, NC, United States
Lers, A., Devmtl., Cell and Molec. Biol. Group, Departments of Botany and Zoology, Duke University, Durham, NC 27708, United States, Dept. Postharvest Sci. Fresh Produce, Volcani Center, Bet-Dagan 50250, Israel
Turpin, D.H., Devmtl., Cell and Molec. Biol. Group, Departments of Botany and Zoology, Duke University, Durham, NC 27708, United States, Department of Biology, Queen's University, Kingston, Ont. K7L 3N6, Canada
Gillham, N.W., Devmtl., Cell and Molec. Biol. Group, Departments of Botany and Zoology, Duke University, Durham, NC 27708, United States
Boynton, J.E., Devmtl., Cell and Molec. Biol. Group, Departments of Botany and Zoology, Duke University, Durham, NC 27708, United States, DCMB Group, LSRC Building, Duke University, Durham, NC 27708-1000, United States
Osmond, C.B., Devmtl., Cell and Molec. Biol. Group, Departments of Botany and Zoology, Duke University, Durham, NC 27708, United States, Photobioenergetics Group, Institute of Advanced Studies, Australian National University, Canberra, ACT 2601, Australia
The effects of introduced chloroplast gene mutations affecting D1 synthesis, turnover and function on photosynthesis, growth and competitive ability were examined in autotrophic cultures of Chlamydomonas reinhardtii (Chlorophyta) adapted to low or high irradiance. Few discernible effects were evident when the mutants were grown in low light (LL, 70 μmol m-2 s-1). The herbicide-resistant psbA mutation Ser264 → Ala (dr) slowed electron transfer and accelerated D1 degradation in cells grown under high light (HL, 600 μmol m-2 S-1). The maximum rate of light-and CO2-saturated photosynthesis, cell growth rate and competitive ability in the dr mutant were reduced compared to wild type under HL. However, the wild-type rate of D1 synthesis in dr was adequate to compensate for accelerated D1 degradation. 16S rRNA mutations conferring resistance to streptomycin and spectinomycin (spr/sr) that altered chloroplast ribosome structure and assembly were used to inhibit chloroplast protein synthesis. In spr/sr cells grown under HL, D1 synthesis was reduced by 40-60% compared to wild type and D1 degradation was accelerated, leading to a 4-fold reduction in D1 pool size. The reduced D1 levels were accompanied by an elevation of F(o) and a decline in F(v)/F(m), quantum yield and maximum rate of CO2-saturated photosynthesis. Chemostat experiments showed that the growth rate and competitive ability of spr/sr were reduced against both wild type and dr.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
dr and spr/sr mutations of Chlamydomonas reinhardtii affecting D1 protein function and synthesis define two independent steps leading to chronic photoinhibition and confer differential fitness
20
Heifetz, P.B., Devmtl., Cell and Molec. Biol. Group, Departments of Botany and Zoology, Duke University, Durham, NC 27708, United States, Novartis Crop Protection Inc., Biotechnology and Genomics Center, 3054 Cornwallis Road, Research Triangle Park, NC, United States
Lers, A., Devmtl., Cell and Molec. Biol. Group, Departments of Botany and Zoology, Duke University, Durham, NC 27708, United States, Dept. Postharvest Sci. Fresh Produce, Volcani Center, Bet-Dagan 50250, Israel
Turpin, D.H., Devmtl., Cell and Molec. Biol. Group, Departments of Botany and Zoology, Duke University, Durham, NC 27708, United States, Department of Biology, Queen's University, Kingston, Ont. K7L 3N6, Canada
Gillham, N.W., Devmtl., Cell and Molec. Biol. Group, Departments of Botany and Zoology, Duke University, Durham, NC 27708, United States
Boynton, J.E., Devmtl., Cell and Molec. Biol. Group, Departments of Botany and Zoology, Duke University, Durham, NC 27708, United States, DCMB Group, LSRC Building, Duke University, Durham, NC 27708-1000, United States
Osmond, C.B., Devmtl., Cell and Molec. Biol. Group, Departments of Botany and Zoology, Duke University, Durham, NC 27708, United States, Photobioenergetics Group, Institute of Advanced Studies, Australian National University, Canberra, ACT 2601, Australia
dr and spr/sr mutations of Chlamydomonas reinhardtii affecting D1 protein function and synthesis define two independent steps leading to chronic photoinhibition and confer differential fitness
The effects of introduced chloroplast gene mutations affecting D1 synthesis, turnover and function on photosynthesis, growth and competitive ability were examined in autotrophic cultures of Chlamydomonas reinhardtii (Chlorophyta) adapted to low or high irradiance. Few discernible effects were evident when the mutants were grown in low light (LL, 70 μmol m-2 s-1). The herbicide-resistant psbA mutation Ser264 → Ala (dr) slowed electron transfer and accelerated D1 degradation in cells grown under high light (HL, 600 μmol m-2 S-1). The maximum rate of light-and CO2-saturated photosynthesis, cell growth rate and competitive ability in the dr mutant were reduced compared to wild type under HL. However, the wild-type rate of D1 synthesis in dr was adequate to compensate for accelerated D1 degradation. 16S rRNA mutations conferring resistance to streptomycin and spectinomycin (spr/sr) that altered chloroplast ribosome structure and assembly were used to inhibit chloroplast protein synthesis. In spr/sr cells grown under HL, D1 synthesis was reduced by 40-60% compared to wild type and D1 degradation was accelerated, leading to a 4-fold reduction in D1 pool size. The reduced D1 levels were accompanied by an elevation of F(o) and a decline in F(v)/F(m), quantum yield and maximum rate of CO2-saturated photosynthesis. Chemostat experiments showed that the growth rate and competitive ability of spr/sr were reduced against both wild type and dr.
Scientific Publication
You may also be interested in