נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Physiological characterization and comparative transcriptome analysis of white and green leaves of ananas comosus var. bracteatus
Year:
2017
Source of publication :
PLoS ONE
Authors :
קנקלה, סורפת'רודו
;
.
Volume :
12
Co-Authors:
Li, X., College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China
Kanakala, S., Institute of Plant Protection, Agricultural Research Organization, Volcani Center, Beit Dagan, Israel
He, Y., Horticultural Biotechnology College of South China Agricultural University, Guangzhou, Guangdong, China
Zhong, X., College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China
Yu, S., College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China
Li, R., College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China
Sun, L., College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China
Ma, J., College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
Leaf coloration is one of the most important and attractive characteristics of Ananas comosus var. bracteatus. The chimeric character is not stable during the in vitro tissue culturing. Many regenerated plants lost economic values for the loss of the chimeric character of leaves. In order to reveal the molecular mechanisms involved in the albino phenotype of the leaf cells, the physiological and transcriptional differences between complete white (CWh) and green (CGr) leaf cells of A. comosus var. bracteatus were analyzed. A total of 1,431 differentially expressed unigenes (DEGs) in CGr and CWh leaves were identified using RNAseq. A comparison to the COG, GO and KEGG annotations revealed DEGs involved in chlorophyll biosynthesis, chloroplast development and photosynthesis. Furthermore, the measurement of main precursors of chlorophyll in the CWh leaves confirmed that the ratelimiting step in chlorophyll biosynthesis, and thus the cause of the albino phenotype of the white cells, was the conversion of pyrrole porphobilinogen (PBG) to uroporphyrinogen III (Uro III). The enzyme activity of porphobilinogen deaminase (PBGD) and uroporporphyrinogn III synthase (UROS), which catalyze the transition of PBG to Uro III, was significantly decreased in the CWh leaves. Our data showed the transcriptional differences between the CWh and CGr plants and characterized key steps in chlorophyll biosynthesis of the CWh leaves. These results contribute to our understanding of the mechanisms and regulation of pigment biosynthesis in the CWh leaf cells of A. comosus var. bracteatus. © 2017 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Note:
Related Files :
עוד תגיות
תוכן קשור
More details
DOI :
10.1371/journal.pone.0169838
Article number:
169838
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22466
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:52
You may also be interested in
Scientific Publication
Physiological characterization and comparative transcriptome analysis of white and green leaves of ananas comosus var. bracteatus
12
Li, X., College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China
Kanakala, S., Institute of Plant Protection, Agricultural Research Organization, Volcani Center, Beit Dagan, Israel
He, Y., Horticultural Biotechnology College of South China Agricultural University, Guangzhou, Guangdong, China
Zhong, X., College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China
Yu, S., College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China
Li, R., College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China
Sun, L., College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China
Ma, J., College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China
Physiological characterization and comparative transcriptome analysis of white and green leaves of ananas comosus var. bracteatus
Leaf coloration is one of the most important and attractive characteristics of Ananas comosus var. bracteatus. The chimeric character is not stable during the in vitro tissue culturing. Many regenerated plants lost economic values for the loss of the chimeric character of leaves. In order to reveal the molecular mechanisms involved in the albino phenotype of the leaf cells, the physiological and transcriptional differences between complete white (CWh) and green (CGr) leaf cells of A. comosus var. bracteatus were analyzed. A total of 1,431 differentially expressed unigenes (DEGs) in CGr and CWh leaves were identified using RNAseq. A comparison to the COG, GO and KEGG annotations revealed DEGs involved in chlorophyll biosynthesis, chloroplast development and photosynthesis. Furthermore, the measurement of main precursors of chlorophyll in the CWh leaves confirmed that the ratelimiting step in chlorophyll biosynthesis, and thus the cause of the albino phenotype of the white cells, was the conversion of pyrrole porphobilinogen (PBG) to uroporphyrinogen III (Uro III). The enzyme activity of porphobilinogen deaminase (PBGD) and uroporporphyrinogn III synthase (UROS), which catalyze the transition of PBG to Uro III, was significantly decreased in the CWh leaves. Our data showed the transcriptional differences between the CWh and CGr plants and characterized key steps in chlorophyll biosynthesis of the CWh leaves. These results contribute to our understanding of the mechanisms and regulation of pigment biosynthesis in the CWh leaf cells of A. comosus var. bracteatus. © 2017 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Scientific Publication
You may also be interested in