אסיף מאגר המחקר החקלאי

Agrobacterium mediated transformation of lisianthus (Eustoma grandiflorum)

Year:

1997

Source of publication :

Authors :

Volume :

447

Co-Authors:

Hecht, M., Department of Ornamental Horticulture, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel, Department of Genetics, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Ecker, R., Department of Ornamental Horticulture, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Stav, R., Department of Ornamental Horticulture, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Watad, A.A., Department of Ornamental Horticulture, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Hirschberg, J., Department of Genetics, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Mann, V., Department of Genetics, Hebrew University of Jerusalem, Jerusalem 91904, Israel

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Abstract:

We have developed an efficient technique for shoot regeneration of lisianthus from leaf explants by direct organogenesis. Transformation experiments with DNA were carried out using Agrobacterium tumefaciens carrying the Ti plasmid pBI121 which contained the genes NPTII and ((3-glucuronidase) GUS fused to the 35S promoter of the CaMV. Kanamycin-resistant shoots of lisianthus were regenerated on Murashige and Skoog (MS) medium with 6-Benzylaminopurine (BA) showed GUS activity, as determined by the appearance of a blue precipitate after incubation with the substrate X-Glue (5-bromo-4-chloro-3-indolyl glucuronide).

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