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פותח על ידי קלירמאש פתרונות בע"מ -
Intron retention is a major phenomenon in alternative splicing in Arabidopsis
Year:
2004
Source of publication :
Plant Journal
Authors :
אופיר, רון
;
.
Volume :
39
Co-Authors:
Ner-Gaon, H., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Halachmi, R., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Savaldi-Goldstein, S., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Rubin, E., Bioinformatics Unit, Department of Biological Services, Weizmann Institute of Science, Rehovot 76100, Israel
Ophir, R., Bioinformatics Unit, Department of Biological Services, Weizmann Institute of Science, Rehovot 76100, Israel
Fluhr, R., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Facilitators :
From page:
877
To page:
885
(
Total pages:
9
)
Abstract:
Alternative splicing (AS) combines different transcript splice junctions that result in transcripts with shuffled exons, alternative 5′ or 3′ splicing sites, retained introns and different transcript termini. In this way, multiple mRNA species and proteins can be created from a single gene expanding the potential informational content of eukaryotic genomes. Search algorithms of AS forms in a variety of Arabidopsis databases showed they contained an unusually high fraction of retained introns (above 30%), compared with 10% that was reported for humans. The preponderance of retained introns (65%) were either part of open reading frames, present in the UTR region or present as the last intron in the transcript, indicating that their occurrence would not participate in non-sense-mediated decay. Interestingly, the functional distribution of the transcripts with retained introns is skewed towards stress and external/internal stimuli-related functions. A sampling of the alternative transcripts with retained introns were confirmed by RT-PCR and were shown to co-purify with polyribosomes, indicating their nuclear export. Thus, retained introns are a prominent feature of AS in Arabidopsis and as such may play a regulatory function.
Note:
Related Files :
arabidopsis
bioinformatics
Genetics
Nucleic acids
Plants
proteins
RNA
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1365-313X.2004.02172.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22512
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:52
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Scientific Publication
Intron retention is a major phenomenon in alternative splicing in Arabidopsis
39
Ner-Gaon, H., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Halachmi, R., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Savaldi-Goldstein, S., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Rubin, E., Bioinformatics Unit, Department of Biological Services, Weizmann Institute of Science, Rehovot 76100, Israel
Ophir, R., Bioinformatics Unit, Department of Biological Services, Weizmann Institute of Science, Rehovot 76100, Israel
Fluhr, R., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Intron retention is a major phenomenon in alternative splicing in Arabidopsis
Alternative splicing (AS) combines different transcript splice junctions that result in transcripts with shuffled exons, alternative 5′ or 3′ splicing sites, retained introns and different transcript termini. In this way, multiple mRNA species and proteins can be created from a single gene expanding the potential informational content of eukaryotic genomes. Search algorithms of AS forms in a variety of Arabidopsis databases showed they contained an unusually high fraction of retained introns (above 30%), compared with 10% that was reported for humans. The preponderance of retained introns (65%) were either part of open reading frames, present in the UTR region or present as the last intron in the transcript, indicating that their occurrence would not participate in non-sense-mediated decay. Interestingly, the functional distribution of the transcripts with retained introns is skewed towards stress and external/internal stimuli-related functions. A sampling of the alternative transcripts with retained introns were confirmed by RT-PCR and were shown to co-purify with polyribosomes, indicating their nuclear export. Thus, retained introns are a prominent feature of AS in Arabidopsis and as such may play a regulatory function.
Scientific Publication
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