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פותח על ידי קלירמאש פתרונות בע"מ -
Limited DNA elimination from the irradiated potato parent in fusion products of albino Lycopersicon esculentum and Solanum tuberosum
Year:
1991
Source of publication :
Theoretical and Applied Genetics
Authors :
זלצר, אהרון
;
.
Volume :
83
Co-Authors:
Wolters, A.M.A., Department of Genetics, Wageningen Agricultural University, Dreijenlaan 2, Wageningen, NL-6703 HA, Netherlands
Schoenmakers, H.C.H., Department of Genetics, Wageningen Agricultural University, Dreijenlaan 2, Wageningen, NL-6703 HA, Netherlands
van der Meulen-Muisers, J.J.M., Department of Genetics, Wageningen Agricultural University, Dreijenlaan 2, Wageningen, NL-6703 HA, Netherlands
van der Knaap, E., Department of Genetics, Wageningen Agricultural University, Dreijenlaan 2, Wageningen, NL-6703 HA, Netherlands
Derks, E.H.M., Centre for Plant Breeding and Reproduction Research CPRO, P.O. Box 16, Wageningen, NL-6700 AA, Netherlands
Koornneef, M., Department of Genetics, Wageningen Agricultural University, Dreijenlaan 2, Wageningen, NL-6703 HA, Netherlands
Zelcer, A., Division of Genetics and Plant Breeding, Institute of Field and Garden Crops, Volcani Centre, Agricultural Research Organization, Bet-Dagan, 50250, Israel
Facilitators :
From page:
225
To page:
232
(
Total pages:
8
)
Abstract:
This paper describes the analysis of the elimination of potato DNA from potato-tomato somatic cell hybrids. The hybrids were obtained by fusion of protoplasts of a cytoplasmic albino tomato genotype with leaf mesophyll protoplasts of a potato genotype carrying the β-glucuronidase (GUS) gene of Escherichia coli. The potato protoplasts were either isolated from unirradiated plants or from plants irradiated with 50 or 500 Gy of γ-rays. Green calli were selected as putative fusion products. The hybridity of these calli was confirmed by isoenzyme analysis. All of the green calli tested contained a potato-specific chloroplast DNA restriction fragment, and most of the calli analysed were positive for β-glucuronidase activity. In 72 of the hybrid calli we determined the percentage of potato nuclear DNA using species-specific probes. All of the tested green calli contained a considerable amount of potato genomic DNA, irrespective of the dose of irradiation of the potato parent. The limited degree of potato DNA elimination and the absence of true cybrids are discussed. © 1991 Springer-Verlag.
Note:
Related Files :
Asymmetric somatic hybrids
Gamma irradiation
Plastome-genome interaction
potato
tomato
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF00226255
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22534
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:52
Scientific Publication
Limited DNA elimination from the irradiated potato parent in fusion products of albino Lycopersicon esculentum and Solanum tuberosum
83
Wolters, A.M.A., Department of Genetics, Wageningen Agricultural University, Dreijenlaan 2, Wageningen, NL-6703 HA, Netherlands
Schoenmakers, H.C.H., Department of Genetics, Wageningen Agricultural University, Dreijenlaan 2, Wageningen, NL-6703 HA, Netherlands
van der Meulen-Muisers, J.J.M., Department of Genetics, Wageningen Agricultural University, Dreijenlaan 2, Wageningen, NL-6703 HA, Netherlands
van der Knaap, E., Department of Genetics, Wageningen Agricultural University, Dreijenlaan 2, Wageningen, NL-6703 HA, Netherlands
Derks, E.H.M., Centre for Plant Breeding and Reproduction Research CPRO, P.O. Box 16, Wageningen, NL-6700 AA, Netherlands
Koornneef, M., Department of Genetics, Wageningen Agricultural University, Dreijenlaan 2, Wageningen, NL-6703 HA, Netherlands
Zelcer, A., Division of Genetics and Plant Breeding, Institute of Field and Garden Crops, Volcani Centre, Agricultural Research Organization, Bet-Dagan, 50250, Israel
Limited DNA elimination from the irradiated potato parent in fusion products of albino Lycopersicon esculentum and Solanum tuberosum
This paper describes the analysis of the elimination of potato DNA from potato-tomato somatic cell hybrids. The hybrids were obtained by fusion of protoplasts of a cytoplasmic albino tomato genotype with leaf mesophyll protoplasts of a potato genotype carrying the β-glucuronidase (GUS) gene of Escherichia coli. The potato protoplasts were either isolated from unirradiated plants or from plants irradiated with 50 or 500 Gy of γ-rays. Green calli were selected as putative fusion products. The hybridity of these calli was confirmed by isoenzyme analysis. All of the green calli tested contained a potato-specific chloroplast DNA restriction fragment, and most of the calli analysed were positive for β-glucuronidase activity. In 72 of the hybrid calli we determined the percentage of potato nuclear DNA using species-specific probes. All of the tested green calli contained a considerable amount of potato genomic DNA, irrespective of the dose of irradiation of the potato parent. The limited degree of potato DNA elimination and the absence of true cybrids are discussed. © 1991 Springer-Verlag.
Scientific Publication
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