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פותח על ידי קלירמאש פתרונות בע"מ -
Studying the supramolecular organization of photosynthetic membranes within freeze-fractured leaf tissues by cryo-scanning electron microscopy
Year:
2016
Source of publication :
Journal of Visualized Experiments
Authors :
חרובי, דנה
;
.
Volume :
2016
Co-Authors:
Charuvi, D., Department of Biological Chemistry, Weizmann Institute of Science, Israel, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Israel
Nevo, R., Department of Biological Chemistry, Weizmann Institute of Science, Israel
Kaplan-Ashiri, I., Department of Chemical Research Support, Weizmann Institute of Science, Israel
Shimoni, E., Department of Chemical Research Support, Weizmann Institute of Science, Israel
Reich, Z., Department of Biological Chemistry, Weizmann Institute of Science, Israel
Facilitators :
From page:
0
To page:
0
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Total pages:
1
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Abstract:
Cryo-scanning electron microscopy (SEM) of freeze-fractured samples allows investigation of biological structures at near native conditions. Here, we describe a technique for studying the supramolecular organization of photosynthetic (thylakoid) membranes within leaf samples. This is achieved by high-pressure freezing of leaf tissues, freeze-fracturing, double-layer coating and finally cryo-SEM imaging. Use of the double-layer coating method allows acquiring high magnification (>100,000X) images with minimal beam damage to the frozen-hydrated samples as well as minimal charging effects. Using the described procedures we investigated the alterations in supramolecular distribution of photosystem and lightharvesting antenna protein complexes that take place during dehydration of the resurrection plant Craterostigma pumilum, in situ. © 2016 Journal of Visualized Experiments.
Related Files :
Exoplasmic fracture face
Issue 112
plant biology
resurrection plants
Supramolecular organization
thylakoid membrane
עוד תגיות
תוכן קשור
More details
DOI :
10.3791/54066
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
וידאו
;
.
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22552
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:52
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Scientific Publication
Studying the supramolecular organization of photosynthetic membranes within freeze-fractured leaf tissues by cryo-scanning electron microscopy
2016
Charuvi, D., Department of Biological Chemistry, Weizmann Institute of Science, Israel, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Israel
Nevo, R., Department of Biological Chemistry, Weizmann Institute of Science, Israel
Kaplan-Ashiri, I., Department of Chemical Research Support, Weizmann Institute of Science, Israel
Shimoni, E., Department of Chemical Research Support, Weizmann Institute of Science, Israel
Reich, Z., Department of Biological Chemistry, Weizmann Institute of Science, Israel
Studying the supramolecular organization of photosynthetic membranes within freeze-fractured leaf tissues by cryo-scanning electron microscopy
Cryo-scanning electron microscopy (SEM) of freeze-fractured samples allows investigation of biological structures at near native conditions. Here, we describe a technique for studying the supramolecular organization of photosynthetic (thylakoid) membranes within leaf samples. This is achieved by high-pressure freezing of leaf tissues, freeze-fracturing, double-layer coating and finally cryo-SEM imaging. Use of the double-layer coating method allows acquiring high magnification (>100,000X) images with minimal beam damage to the frozen-hydrated samples as well as minimal charging effects. Using the described procedures we investigated the alterations in supramolecular distribution of photosystem and lightharvesting antenna protein complexes that take place during dehydration of the resurrection plant Craterostigma pumilum, in situ. © 2016 Journal of Visualized Experiments.

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Scientific Publication
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