חיפוש מתקדם
Journal of Visualized Experiments
Charuvi, D., Department of Biological Chemistry, Weizmann Institute of Science, Israel, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Israel
Nevo, R., Department of Biological Chemistry, Weizmann Institute of Science, Israel
Kaplan-Ashiri, I., Department of Chemical Research Support, Weizmann Institute of Science, Israel
Shimoni, E., Department of Chemical Research Support, Weizmann Institute of Science, Israel
Reich, Z., Department of Biological Chemistry, Weizmann Institute of Science, Israel
Cryo-scanning electron microscopy (SEM) of freeze-fractured samples allows investigation of biological structures at near native conditions. Here, we describe a technique for studying the supramolecular organization of photosynthetic (thylakoid) membranes within leaf samples. This is achieved by high-pressure freezing of leaf tissues, freeze-fracturing, double-layer coating and finally cryo-SEM imaging. Use of the double-layer coating method allows acquiring high magnification (>100,000X) images with minimal beam damage to the frozen-hydrated samples as well as minimal charging effects. Using the described procedures we investigated the alterations in supramolecular distribution of photosystem and lightharvesting antenna protein complexes that take place during dehydration of the resurrection plant Craterostigma pumilum, in situ. © 2016 Journal of Visualized Experiments.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Studying the supramolecular organization of photosynthetic membranes within freeze-fractured leaf tissues by cryo-scanning electron microscopy
2016
Charuvi, D., Department of Biological Chemistry, Weizmann Institute of Science, Israel, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Israel
Nevo, R., Department of Biological Chemistry, Weizmann Institute of Science, Israel
Kaplan-Ashiri, I., Department of Chemical Research Support, Weizmann Institute of Science, Israel
Shimoni, E., Department of Chemical Research Support, Weizmann Institute of Science, Israel
Reich, Z., Department of Biological Chemistry, Weizmann Institute of Science, Israel
Studying the supramolecular organization of photosynthetic membranes within freeze-fractured leaf tissues by cryo-scanning electron microscopy
Cryo-scanning electron microscopy (SEM) of freeze-fractured samples allows investigation of biological structures at near native conditions. Here, we describe a technique for studying the supramolecular organization of photosynthetic (thylakoid) membranes within leaf samples. This is achieved by high-pressure freezing of leaf tissues, freeze-fracturing, double-layer coating and finally cryo-SEM imaging. Use of the double-layer coating method allows acquiring high magnification (>100,000X) images with minimal beam damage to the frozen-hydrated samples as well as minimal charging effects. Using the described procedures we investigated the alterations in supramolecular distribution of photosystem and lightharvesting antenna protein complexes that take place during dehydration of the resurrection plant Craterostigma pumilum, in situ. © 2016 Journal of Visualized Experiments.

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