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פותח על ידי קלירמאש פתרונות בע"מ -
Detection of bean yellow mosaic virus in gladioli corms by the polymerase chain reaction
Year:
1991
Source of publication :
Annals of Applied Biology
Authors :
רוזנר, אריה
;
.
ריגר-שטיין, עדינה
;
.
Volume :
119
Co-Authors:
VUNSH, R., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
ROSNER, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
STEIN, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Facilitators :
From page:
289
To page:
294
(
Total pages:
6
)
Abstract:
The polymerase chain reaction (PCR) method readily detected bean yellow mosaic virus (BYMV) in gladioli leaves, but in initial tests PCR did not detect virus in corm tissue. Extracts of RN A from corm tissue were shown to inhibit the amplification of viral sequences when added to a PCR reaction. An additional purification step for the RNA extracts using a Sephadex G‐50 column eliminated the inhibitory effect and enabled PCR to amplify and detect viral RNA in corm tissue preparations. Copyright © 1991, Wiley Blackwell. All rights reserved
Note:
Related Files :
BYMV
gladioli corms
PCR
virus detection
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1744-7348.1991.tb04867.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22575
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:52
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Scientific Publication
Detection of bean yellow mosaic virus in gladioli corms by the polymerase chain reaction
119
VUNSH, R., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
ROSNER, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
STEIN, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Detection of bean yellow mosaic virus in gladioli corms by the polymerase chain reaction
The polymerase chain reaction (PCR) method readily detected bean yellow mosaic virus (BYMV) in gladioli leaves, but in initial tests PCR did not detect virus in corm tissue. Extracts of RN A from corm tissue were shown to inhibit the amplification of viral sequences when added to a PCR reaction. An additional purification step for the RNA extracts using a Sephadex G‐50 column eliminated the inhibitory effect and enabled PCR to amplify and detect viral RNA in corm tissue preparations. Copyright © 1991, Wiley Blackwell. All rights reserved
Scientific Publication
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