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Annals of Applied Biology
VUNSH, R., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
ROSNER, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
STEIN, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
The polymerase chain reaction (PCR) method readily detected bean yellow mosaic virus (BYMV) in gladioli leaves, but in initial tests PCR did not detect virus in corm tissue. Extracts of RN A from corm tissue were shown to inhibit the amplification of viral sequences when added to a PCR reaction. An additional purification step for the RNA extracts using a Sephadex G‐50 column eliminated the inhibitory effect and enabled PCR to amplify and detect viral RNA in corm tissue preparations. Copyright © 1991, Wiley Blackwell. All rights reserved
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הספר "אוצר וולקני"
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תנאי שימוש
Detection of bean yellow mosaic virus in gladioli corms by the polymerase chain reaction
119
VUNSH, R., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
ROSNER, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
STEIN, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Detection of bean yellow mosaic virus in gladioli corms by the polymerase chain reaction
The polymerase chain reaction (PCR) method readily detected bean yellow mosaic virus (BYMV) in gladioli leaves, but in initial tests PCR did not detect virus in corm tissue. Extracts of RN A from corm tissue were shown to inhibit the amplification of viral sequences when added to a PCR reaction. An additional purification step for the RNA extracts using a Sephadex G‐50 column eliminated the inhibitory effect and enabled PCR to amplify and detect viral RNA in corm tissue preparations. Copyright © 1991, Wiley Blackwell. All rights reserved
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