נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Induction of potato steroidal glycoalkaloid biosynthetic pathway by overexpression of cDNA encoding primary metabolism HMG-CoA reductase and squalene synthase
Year:
2012
Source of publication :
Planta
Authors :
גינזברג, עידית
;
.
Volume :
235
Co-Authors:
Ginzberg, I., Institute of Plant Sciences, ARO, the Volcani Center, 50250 Bet Dagan, Israel
Thippeswamy, M., Institute of Plant Sciences, ARO, the Volcani Center, 50250 Bet Dagan, Israel, Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India
Fogelman, E., Institute of Plant Sciences, ARO, the Volcani Center, 50250 Bet Dagan, Israel
Demirel, U., Institute of Plant Sciences, ARO, the Volcani Center, 50250 Bet Dagan, Israel, Department of Field Crops, Harran University, Sanliurfa 63000, Turkey
Mweetwa, A.M., Department of Horticulture, Virginia Tech Blacksburg, Virginia, United States, University of Zambia, Lusaka, Zambia
Tokuhisa, J., Department of Horticulture, Virginia Tech Blacksburg, Virginia, United States
Veilleux, R.E., Department of Horticulture, Virginia Tech Blacksburg, Virginia, United States
Facilitators :
From page:
1341
To page:
1353
(
Total pages:
13
)
Abstract:
Potato steroidal glycoalkaloids (SGAs) are toxic secondary metabolites whose total content in tubers must be regulated. SGAs are biosynthesized by the sterol branch of the mevalonic acid/isoprenoid pathway. In a previous study, we showed a correlation between SGA levels and the abundance of transcript coding for HMG-CoA reductase 1 (HMG1) and squalene synthase 1 (SQS1) in potato tissues and potato genotypes varying in SGA content. Here, Solanum tuberosum cv. Desirée (low SGA producer) was transformed with a gene construct containing the coding region of either HMG1 or SQS1 of Solanum chacoense Bitt. clone 8380-1, a high SGA producer. SGA levels in transgenic HMG-plants were either greater than (in eight of 14 plants) or no different from untransformed controls, whereas only four of 12 SQS-transgenics had greater SGA levels than control, as determined by HPLC. Quantitative real-time PCR was used to estimate relative steady-state transcript levels of isoprenoid-, steroid-, and SGA-related genes in leaves of the transgenic plants compared to nontransgenic controls. HMG-transgenic plants exhibited increased transcript accumulation of SQS1, sterol C24-methyltransferase type1 (SMT1), and solanidine glycosyltransferase 2 (SGT2), whereas SQS-transgenic plants, had consistently lower transcript levels of HMG1 and variable SMT1 and SGT2 transcript abundance among different transgenics. HMG-transgenic plants exhibited changes in transcript accumulation for some sterol biosynthetic genes as well. Taken together, the data suggest coordinated regulation of isoprenoid metabolism and SGA secondary metabolism. © 2011 Springer-Verlag.
Note:
Related Files :
alpha chaconine
biosynthesis
drug derivative
Genetics
metabolism
Solanum
Solanum tuberosum
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00425-011-1578-6
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22644
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:53
Scientific Publication
Induction of potato steroidal glycoalkaloid biosynthetic pathway by overexpression of cDNA encoding primary metabolism HMG-CoA reductase and squalene synthase
235
Ginzberg, I., Institute of Plant Sciences, ARO, the Volcani Center, 50250 Bet Dagan, Israel
Thippeswamy, M., Institute of Plant Sciences, ARO, the Volcani Center, 50250 Bet Dagan, Israel, Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India
Fogelman, E., Institute of Plant Sciences, ARO, the Volcani Center, 50250 Bet Dagan, Israel
Demirel, U., Institute of Plant Sciences, ARO, the Volcani Center, 50250 Bet Dagan, Israel, Department of Field Crops, Harran University, Sanliurfa 63000, Turkey
Mweetwa, A.M., Department of Horticulture, Virginia Tech Blacksburg, Virginia, United States, University of Zambia, Lusaka, Zambia
Tokuhisa, J., Department of Horticulture, Virginia Tech Blacksburg, Virginia, United States
Veilleux, R.E., Department of Horticulture, Virginia Tech Blacksburg, Virginia, United States
Induction of potato steroidal glycoalkaloid biosynthetic pathway by overexpression of cDNA encoding primary metabolism HMG-CoA reductase and squalene synthase
Potato steroidal glycoalkaloids (SGAs) are toxic secondary metabolites whose total content in tubers must be regulated. SGAs are biosynthesized by the sterol branch of the mevalonic acid/isoprenoid pathway. In a previous study, we showed a correlation between SGA levels and the abundance of transcript coding for HMG-CoA reductase 1 (HMG1) and squalene synthase 1 (SQS1) in potato tissues and potato genotypes varying in SGA content. Here, Solanum tuberosum cv. Desirée (low SGA producer) was transformed with a gene construct containing the coding region of either HMG1 or SQS1 of Solanum chacoense Bitt. clone 8380-1, a high SGA producer. SGA levels in transgenic HMG-plants were either greater than (in eight of 14 plants) or no different from untransformed controls, whereas only four of 12 SQS-transgenics had greater SGA levels than control, as determined by HPLC. Quantitative real-time PCR was used to estimate relative steady-state transcript levels of isoprenoid-, steroid-, and SGA-related genes in leaves of the transgenic plants compared to nontransgenic controls. HMG-transgenic plants exhibited increased transcript accumulation of SQS1, sterol C24-methyltransferase type1 (SMT1), and solanidine glycosyltransferase 2 (SGT2), whereas SQS-transgenic plants, had consistently lower transcript levels of HMG1 and variable SMT1 and SGT2 transcript abundance among different transgenics. HMG-transgenic plants exhibited changes in transcript accumulation for some sterol biosynthetic genes as well. Taken together, the data suggest coordinated regulation of isoprenoid metabolism and SGA secondary metabolism. © 2011 Springer-Verlag.
Scientific Publication
You may also be interested in