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פותח על ידי קלירמאש פתרונות בע"מ -
Proliferation of meristematic clusters in disposable presterilized plastic bioreactors for the large-scale micropropagation of plants
Year:
1998
Authors :
רביב, מיכאל
;
.
Volume :
34
Co-Authors:
Ziv, M., Department of Agricultural Botany, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Ronen, G., Department of Agricultural Botany, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Raviv, M., Department of Agricultural Botany, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Facilitators :
From page:
152
To page:
158
(
Total pages:
7
)
Abstract:
Proliferation of meristematic clusters of several plants in an inexpensive airlift bioreactor system, consisting of a disposable presterilized light transmittable plastic film vessel is described. The optimal shape, size, and structural function of the disposable plastic bioreactor are based on the bubble column and airlift glass bioreactors. The disposable bioreactors are designed in a conical configuration with a single inoculation and harvest port and multiple use dispensing and mixing accessories. Shearing damage and foaming problems known to exist in bioreactors due to the plant's rigid cell wall and size were greatly reduced in the disposable plastic bioreactors. The disposable bioreactors were used for propagule proliferation and growth, using meristem and bud clusters of potato, fern, banana, and gladiolus. The clusters' biomass increased five-to eightfold over a period of 26-30 d, depending on the species. The clusters were separated mechanically by a chopper made of a grid of knives. The chopped propagules were inoculated to agar medium for further growth and developed into transplantable plants. In the case of gladiolus and potato, corms and tubers developed in a sucrose-elevated storage organ induction medium, respectively, after the initial formation of small shoots. The plantlets and storage organs were transplanted to an acclimation greenhouse and continued to grow with a 95-100% survival, depending on the species. Plant development was followed for a period of 16 wk in fern and 12-14 wk in potato, banana, and gladiolus and normal shoot and leaf growth was observed. The feasibility of large-scale liquid cultures for plant micropropagation is discussed.
Note:
Related Files :
Bioreactor
Clusters
Gladiolus
Growth retardants
micropropagation
plant development
plant growth
plant propagation
tubers
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22668
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:53
Scientific Publication
Proliferation of meristematic clusters in disposable presterilized plastic bioreactors for the large-scale micropropagation of plants
34
Ziv, M., Department of Agricultural Botany, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Ronen, G., Department of Agricultural Botany, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Raviv, M., Department of Agricultural Botany, Otto Warburg Ctr. Biotechnol. Agric., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Proliferation of meristematic clusters in disposable presterilized plastic bioreactors for the large-scale micropropagation of plants
Proliferation of meristematic clusters of several plants in an inexpensive airlift bioreactor system, consisting of a disposable presterilized light transmittable plastic film vessel is described. The optimal shape, size, and structural function of the disposable plastic bioreactor are based on the bubble column and airlift glass bioreactors. The disposable bioreactors are designed in a conical configuration with a single inoculation and harvest port and multiple use dispensing and mixing accessories. Shearing damage and foaming problems known to exist in bioreactors due to the plant's rigid cell wall and size were greatly reduced in the disposable plastic bioreactors. The disposable bioreactors were used for propagule proliferation and growth, using meristem and bud clusters of potato, fern, banana, and gladiolus. The clusters' biomass increased five-to eightfold over a period of 26-30 d, depending on the species. The clusters were separated mechanically by a chopper made of a grid of knives. The chopped propagules were inoculated to agar medium for further growth and developed into transplantable plants. In the case of gladiolus and potato, corms and tubers developed in a sucrose-elevated storage organ induction medium, respectively, after the initial formation of small shoots. The plantlets and storage organs were transplanted to an acclimation greenhouse and continued to grow with a 95-100% survival, depending on the species. Plant development was followed for a period of 16 wk in fern and 12-14 wk in potato, banana, and gladiolus and normal shoot and leaf growth was observed. The feasibility of large-scale liquid cultures for plant micropropagation is discussed.
Scientific Publication
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