חיפוש מתקדם
Orlofsky, E., Zuckerberg Institute for Water Research, J. Blaustein Institutes for Desert Research, Ben Gurion University, Midreshet Ben Gurion, Israel
Bernstein, N., Institute of Soil Water and Environmental Sciences, Volcani Center, POB 6, Bet-Dagan, Israel
Sacks, M., Ministry of Agriculture, Extension Service Bureau, Tzfon Yehuda, Israel
Vonshak, A., Zuckerberg Institute for Water Research, J. Blaustein Institutes for Desert Research, Ben Gurion University, Midreshet Ben Gurion, Israel
Benami, M., Zuckerberg Institute for Water Research, J. Blaustein Institutes for Desert Research, Ben Gurion University, Midreshet Ben Gurion, Israel
Kundu, A., Department of Civil and Environmental Engineering, University of California, One Shields Avenue, Davis, CA, United States
Maki, M., Department of Civil and Environmental Engineering, University of California, One Shields Avenue, Davis, CA, United States
Smith, W., Singapore Centre on Environmental Life Sciences Engineering (SCELSE), School of Civil and Environmental Engineering, Nanyang Technological University, Singapore
Wuertz, S., Department of Civil and Environmental Engineering, University of California, One Shields Avenue, Davis, CA, United States, Singapore Centre on Environmental Life Sciences Engineering (SCELSE), School of Civil and Environmental Engineering, Nanyang Technological University, Singapore
Shapiro, K., Department of Pathology, Microbiology and Immunology, University of California, One Shields Avenue, Davis, CA, United States
Gillor, O., Zuckerberg Institute for Water Research, J. Blaustein Institutes for Desert Research, Ben Gurion University, Midreshet Ben Gurion, Israel
To evaluate the impact of treated wastewater (TWW) irrigation for produce safety, field experiments were conducted to compare secondary and tertiary TWW with potable water using tomatoes as a model crop. Human pathogens including a suite of obligate and opportunistic bacterial pathogens (Campylobacter, Pseudomonas, Salmonella, Shigella, Staphylococcus), protozoa (Cryptosporidium and Giardia), and viruses (Adenovirus and Enterovirus) were monitored in two field trials using a combination of microscopic, cultivation-based, and molecular (qPCR) techniques. Results indicate that microbial contamination on the surface of tomatoes was not associated with the source of irrigation waters; fecal indicator bacteria (FIB) contamination was not statistically different on tomatoes irrigated with TWW or potable water. In fact, indicator bacteria testing did not predict the presence of pathogens in any of the matrices tested. Indicator bacteria and the opportunistic pathogens were detected in water, soil and on tomato surfaces from all irrigation treatment schemes, and were positively correlated with duration of time in the field (p<. 0.0001). Pathogen contamination (Cryptosporidium) was detected in secondary TWW (3/14 samples) and on the surface of a TWW irrigated tomato (1/41 samples). Furthermore, the Enterobacteriaceae species in the TWW were markedly different from those found in soil and tomato. The results indicate that (surface drip) irrigation with TWW did not result in the transfer of fecal indicator bacteria or microbial pathogens to the irrigated soil or crop. Moreover, parallel testing for pathogens with traditional culture-based and quantitative PCR indicates that specific and rapid molecular testing of pathogens appears to be a more appropriate strategy than fecal indicator testing for the determination of reclaimed water safety. © 2015 Elsevier B.V.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Comparable levels of microbial contamination in soil and on tomato crops after drip irrigation with treated wastewater or potable water
215
Orlofsky, E., Zuckerberg Institute for Water Research, J. Blaustein Institutes for Desert Research, Ben Gurion University, Midreshet Ben Gurion, Israel
Bernstein, N., Institute of Soil Water and Environmental Sciences, Volcani Center, POB 6, Bet-Dagan, Israel
Sacks, M., Ministry of Agriculture, Extension Service Bureau, Tzfon Yehuda, Israel
Vonshak, A., Zuckerberg Institute for Water Research, J. Blaustein Institutes for Desert Research, Ben Gurion University, Midreshet Ben Gurion, Israel
Benami, M., Zuckerberg Institute for Water Research, J. Blaustein Institutes for Desert Research, Ben Gurion University, Midreshet Ben Gurion, Israel
Kundu, A., Department of Civil and Environmental Engineering, University of California, One Shields Avenue, Davis, CA, United States
Maki, M., Department of Civil and Environmental Engineering, University of California, One Shields Avenue, Davis, CA, United States
Smith, W., Singapore Centre on Environmental Life Sciences Engineering (SCELSE), School of Civil and Environmental Engineering, Nanyang Technological University, Singapore
Wuertz, S., Department of Civil and Environmental Engineering, University of California, One Shields Avenue, Davis, CA, United States, Singapore Centre on Environmental Life Sciences Engineering (SCELSE), School of Civil and Environmental Engineering, Nanyang Technological University, Singapore
Shapiro, K., Department of Pathology, Microbiology and Immunology, University of California, One Shields Avenue, Davis, CA, United States
Gillor, O., Zuckerberg Institute for Water Research, J. Blaustein Institutes for Desert Research, Ben Gurion University, Midreshet Ben Gurion, Israel
Comparable levels of microbial contamination in soil and on tomato crops after drip irrigation with treated wastewater or potable water
To evaluate the impact of treated wastewater (TWW) irrigation for produce safety, field experiments were conducted to compare secondary and tertiary TWW with potable water using tomatoes as a model crop. Human pathogens including a suite of obligate and opportunistic bacterial pathogens (Campylobacter, Pseudomonas, Salmonella, Shigella, Staphylococcus), protozoa (Cryptosporidium and Giardia), and viruses (Adenovirus and Enterovirus) were monitored in two field trials using a combination of microscopic, cultivation-based, and molecular (qPCR) techniques. Results indicate that microbial contamination on the surface of tomatoes was not associated with the source of irrigation waters; fecal indicator bacteria (FIB) contamination was not statistically different on tomatoes irrigated with TWW or potable water. In fact, indicator bacteria testing did not predict the presence of pathogens in any of the matrices tested. Indicator bacteria and the opportunistic pathogens were detected in water, soil and on tomato surfaces from all irrigation treatment schemes, and were positively correlated with duration of time in the field (p<. 0.0001). Pathogen contamination (Cryptosporidium) was detected in secondary TWW (3/14 samples) and on the surface of a TWW irrigated tomato (1/41 samples). Furthermore, the Enterobacteriaceae species in the TWW were markedly different from those found in soil and tomato. The results indicate that (surface drip) irrigation with TWW did not result in the transfer of fecal indicator bacteria or microbial pathogens to the irrigated soil or crop. Moreover, parallel testing for pathogens with traditional culture-based and quantitative PCR indicates that specific and rapid molecular testing of pathogens appears to be a more appropriate strategy than fecal indicator testing for the determination of reclaimed water safety. © 2015 Elsevier B.V.
Scientific Publication
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