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פותח על ידי קלירמאש פתרונות בע"מ -
Mutagenesis of an AUG codon in the adeno-associated virus rep gene: Effects on viral DNA replication
Year:
1989
Source of publication :
Virology
Authors :
צ'חנובסקי, נור
;
.
Volume :
173
Co-Authors:
Chejanovsky, N., Laboratory of Molecular and Cellular Biology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Building 8, Room 304, Bethesda, MD 20892, United States
Carter, B.J., Laboratory of Molecular and Cellular Biology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Building 8, Room 304, Bethesda, MD 20892, United States
Facilitators :
From page:
120
To page:
128
(
Total pages:
9
)
Abstract:
The adeno-associated virus (AAV) rep gene is transcribed from two promoters, pa and p19, which code for two overlapping families of rep proteins. The proteins coded by p5 transcripts contain an amino-terminal domain not present in the proteins coded by p19 transcripts. The rep gene is required for AAV DNA replication and also mediates pleiotropic effects in positive and negative regulation of expression of genes driven by either AAV or heterologous promoters. All three functions require rep proteins coded by p5 transcripts. The functions of the rep proteins coded by the p,9 transcripts could not be independently discerned since the coding region for these proteins and the p,9 promoter are embedded within the coding region of the p5 transcript unit. We describe here an AAV mutant in which the putative AUG initiation codon in the p,9 transcripts was altered and which did not express p19-coded rep proteins. This mutant exhibited normal AAV duplex RF DNA replication but was deficient in accumulation of AAV single-stranded progeny DNA and infectious AAV particles. This mutant defines a novel phenotype for a rep gene mutation and suggests a role for the rep proteins in the generation or accumulation of the viral SS DNA. Moreover this mutant distinguishes two different functions of the rep protein(s) in accumulation of the RF molecules and accumulation or processing of the SS DNA molecules, respectively. © 1989.
Note:
Related Files :
Base Sequence
Cell Line, Transformed
genetic engineering
mutation
phenotype
Plasmid
virus gene
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0042-6822(89)90227-4
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22720
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:53
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Scientific Publication
Mutagenesis of an AUG codon in the adeno-associated virus rep gene: Effects on viral DNA replication
173
Chejanovsky, N., Laboratory of Molecular and Cellular Biology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Building 8, Room 304, Bethesda, MD 20892, United States
Carter, B.J., Laboratory of Molecular and Cellular Biology, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Building 8, Room 304, Bethesda, MD 20892, United States
Mutagenesis of an AUG codon in the adeno-associated virus rep gene: Effects on viral DNA replication
The adeno-associated virus (AAV) rep gene is transcribed from two promoters, pa and p19, which code for two overlapping families of rep proteins. The proteins coded by p5 transcripts contain an amino-terminal domain not present in the proteins coded by p19 transcripts. The rep gene is required for AAV DNA replication and also mediates pleiotropic effects in positive and negative regulation of expression of genes driven by either AAV or heterologous promoters. All three functions require rep proteins coded by p5 transcripts. The functions of the rep proteins coded by the p,9 transcripts could not be independently discerned since the coding region for these proteins and the p,9 promoter are embedded within the coding region of the p5 transcript unit. We describe here an AAV mutant in which the putative AUG initiation codon in the p,9 transcripts was altered and which did not express p19-coded rep proteins. This mutant exhibited normal AAV duplex RF DNA replication but was deficient in accumulation of AAV single-stranded progeny DNA and infectious AAV particles. This mutant defines a novel phenotype for a rep gene mutation and suggests a role for the rep proteins in the generation or accumulation of the viral SS DNA. Moreover this mutant distinguishes two different functions of the rep protein(s) in accumulation of the RF molecules and accumulation or processing of the SS DNA molecules, respectively. © 1989.
Scientific Publication
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