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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Defective RNA molecules associated with citrus tristeza virus
Year:
1995
Source of publication :
Virology
Authors :
בר-יוסף, משה
;
.
גפני, רון
;
.
מוואסי, מוניר
;
.
מיטקיבסקה, אליזבטה
;
.
Volume :
208
Co-Authors:

Mawassi, M., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan 50250, Israel,
Karasev, A.V.
Mietkiewska, E., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan 50250, Israel,
Gafny, R., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan 50250, Israel,
Lee, R.F.
Dawson, W.O.
Bar- Joseph, M., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan 50250, Israel,
 

Facilitators :
From page:
383
To page:
387
(
Total pages:
5
)
Abstract:
Preparations of single-stranded (ss) RNA extracted from particles of the Israeli VT strain of citrus tristeza virus (CTV-VT), and ss- and double- stranded (ds) RNA preparations extracted from infected Alemow (Citrus macrophylla) plants, contained a population of molecules with features that suggest that they are defective RNAs. The prototype of 2424 nt was cloned and sequenced and was found to be composed of two genomic regions corresponding to the 5' (1151 nt) and the 3' (1259 nt) termini of the genomic CTV-RNA, with two perfect direct repeats of eight nucleotides of unknown origin at the junction site. Northern hybridization analysis demonstrated that this 2.4-kb defective RNA is an abundant species among the other CTV-specific ss- and ds- RNAs in infected plants. The 2.4-kb RNA was found encapsidated by the CTV coat protein indicating that the CTV origin of assembly is located close to the 5' or 3' terminus. This is the first defective RNA to be reported for a member of the closterovirus group.
Note:
Related Files :
Base Sequence
Citrus macrophylla
Citrus tristeza virus
Closterovirus
Genome, Viral
Molecular Sequence Data
virus gene
virus RNA
עוד תגיות
תוכן קשור
More details
DOI :
10.1006/viro.1995.1165
Article number:
3000
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22722
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:53
You may also be interested in
Scientific Publication
Defective RNA molecules associated with citrus tristeza virus
208

Mawassi, M., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan 50250, Israel,
Karasev, A.V.
Mietkiewska, E., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan 50250, Israel,
Gafny, R., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan 50250, Israel,
Lee, R.F.
Dawson, W.O.
Bar- Joseph, M., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan 50250, Israel,
 

Defective RNA molecules associated with citrus tristeza virus
Preparations of single-stranded (ss) RNA extracted from particles of the Israeli VT strain of citrus tristeza virus (CTV-VT), and ss- and double- stranded (ds) RNA preparations extracted from infected Alemow (Citrus macrophylla) plants, contained a population of molecules with features that suggest that they are defective RNAs. The prototype of 2424 nt was cloned and sequenced and was found to be composed of two genomic regions corresponding to the 5' (1151 nt) and the 3' (1259 nt) termini of the genomic CTV-RNA, with two perfect direct repeats of eight nucleotides of unknown origin at the junction site. Northern hybridization analysis demonstrated that this 2.4-kb defective RNA is an abundant species among the other CTV-specific ss- and ds- RNAs in infected plants. The 2.4-kb RNA was found encapsidated by the CTV coat protein indicating that the CTV origin of assembly is located close to the 5' or 3' terminus. This is the first defective RNA to be reported for a member of the closterovirus group.
Scientific Publication
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