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פותח על ידי קלירמאש פתרונות בע"מ -
Simple hand-held devices for the efficient infection of plants with viral-encoding contructs by particle bombardment
Year:
1997
Source of publication :
Journal of Virological Methods
Authors :
אלמן, חסיה
;
.
גאבה, ויקטור
;
.
גל-און, עמית
;
.
מאירי, אתי
;
.
Volume :
64
Co-Authors:
Gal-On, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Meiri, E., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Elman, C., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Gray, D.J., Ctrl. Florida Res. and Educ. Center, IFAS, University of Florida, 5336 University Avenue, Leesburg, FL 34748-8232, United States
Gaba, V., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Facilitators :
From page:
103
To page:
110
(
Total pages:
8
)
Abstract:
An efficient method for infection of plants with a cloned potyvirus by particle bombardment has been described (Gal-On et al., 1995). A simplified method is described now whereby a vacuum chamber and helium propulsive gas are not required to achieve a high efficiency of infection. The new device - the 'HandGun' - is hand-held, and easily constructed from readily available materials. With this technique it is possible to bombard soft plants and seedlings that do not survive particle bombardment by other devices. Bombardment of C. pepo plants with a full length clone of zucchini yellow mosaic potyvirus results in approximately 100% infection at 100 pg cDNA per plant using air or helium to propel the microprojectiles. The HandGun is 105-fold more efficient than mechanical inoculation. Tungsten and gold were found to be the most efficient materials tested for use as microprojectiles. Crude extracts of plasmids from E. coli were found to be effective, as well as column-purified cDNA. A functional, simple version of the HandGun - 'the Blowpipe' - was also constructed, which does not require an electrically controlled valve. Plants can be inoculated with plant viruses from sap with the HandGun.
Note:
Related Files :
infectious clone
Inoculation
plant virus inoculation
vegetables
virus infection
virus particle
Zucchini yellow mosaic virus
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/S0166-0934(96)02146-5
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22761
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:54
Scientific Publication
Simple hand-held devices for the efficient infection of plants with viral-encoding contructs by particle bombardment
64
Gal-On, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Meiri, E., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Elman, C., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Gray, D.J., Ctrl. Florida Res. and Educ. Center, IFAS, University of Florida, 5336 University Avenue, Leesburg, FL 34748-8232, United States
Gaba, V., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Simple hand-held devices for the efficient infection of plants with viral-encoding contructs by particle bombardment
An efficient method for infection of plants with a cloned potyvirus by particle bombardment has been described (Gal-On et al., 1995). A simplified method is described now whereby a vacuum chamber and helium propulsive gas are not required to achieve a high efficiency of infection. The new device - the 'HandGun' - is hand-held, and easily constructed from readily available materials. With this technique it is possible to bombard soft plants and seedlings that do not survive particle bombardment by other devices. Bombardment of C. pepo plants with a full length clone of zucchini yellow mosaic potyvirus results in approximately 100% infection at 100 pg cDNA per plant using air or helium to propel the microprojectiles. The HandGun is 105-fold more efficient than mechanical inoculation. Tungsten and gold were found to be the most efficient materials tested for use as microprojectiles. Crude extracts of plasmids from E. coli were found to be effective, as well as column-purified cDNA. A functional, simple version of the HandGun - 'the Blowpipe' - was also constructed, which does not require an electrically controlled valve. Plants can be inoculated with plant viruses from sap with the HandGun.
Scientific Publication
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