נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Photochemical generation of superoxide radical and the cytotoxicity of phthalocyanines
Year:
1985
Authors :
רוזנטל, יונל
;
.
Volume :
48
Co-Authors:
Ben-Hur, E., Department of Radiobiology, Nuclear Research Center-Negev, P.O. Box 9001, Beer-Sheva, Israel
Carmichael, A., Radiation Oncology Branch, Clinical Oncology Program, Division of Cancer Treatment, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20205, United States
Riesz, P., Department of Food Science, A.R.O. Volcani Center, P.O. Box 6, Bet-Dagan, 50250, Israel
Rosenthal, I., Department of Radiobiology, Nuclear Research Center-Negev, P.O. Box 9001, Beer-Sheva, Israel
Facilitators :
From page:
837
To page:
846
(
Total pages:
10
)
Abstract:
The effect of the central metal atom on the photodynamic activity of phthalocyanine dyes has been estimated by cytotoxicity to cultured Chinese hamster cells. Chloroaluminium phthalocyanine, followed by the Zn- derivate, were found to be the only active dyes. In parallel it was found that visible light (615 ± 10 nm) excitation of phthalocyanines dissolved in dimethylsulphoxide in the presence of oxygen generates superoxide radical anion. O- 2 radicals were spintrapped with 5,5-dimethyl-1-pyrroline-1-oxide (DMPO) and identified by electron spin resonance. The quantum yields for O- 2 generation range from 10-5 (Zn-phthalocyanine) to 4·2 × 10-4 (Ga-phthalocyanine). The efficiency of generating O- 2 was apparently uncorrelated with the phototoxicity of the same dyes. Furthermore, the biological photodamage could not be inhibited by the addition of superoxide dismutase. It is concluded that O- 2 is involved very little, if at all, in the phthalocyanine-induced photo-killing of mammalian cells. © 1985 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
Note:
Related Files :
Animal
Electron Spin Resonance Spectroscopy
free radicals
superoxide dismutase
Superoxide radical anion
עוד תגיות
תוכן קשור
More details
DOI :
10.1080/09553008514551931
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22763
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:54
Scientific Publication
Photochemical generation of superoxide radical and the cytotoxicity of phthalocyanines
48
Ben-Hur, E., Department of Radiobiology, Nuclear Research Center-Negev, P.O. Box 9001, Beer-Sheva, Israel
Carmichael, A., Radiation Oncology Branch, Clinical Oncology Program, Division of Cancer Treatment, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20205, United States
Riesz, P., Department of Food Science, A.R.O. Volcani Center, P.O. Box 6, Bet-Dagan, 50250, Israel
Rosenthal, I., Department of Radiobiology, Nuclear Research Center-Negev, P.O. Box 9001, Beer-Sheva, Israel
Photochemical generation of superoxide radical and the cytotoxicity of phthalocyanines
The effect of the central metal atom on the photodynamic activity of phthalocyanine dyes has been estimated by cytotoxicity to cultured Chinese hamster cells. Chloroaluminium phthalocyanine, followed by the Zn- derivate, were found to be the only active dyes. In parallel it was found that visible light (615 ± 10 nm) excitation of phthalocyanines dissolved in dimethylsulphoxide in the presence of oxygen generates superoxide radical anion. O- 2 radicals were spintrapped with 5,5-dimethyl-1-pyrroline-1-oxide (DMPO) and identified by electron spin resonance. The quantum yields for O- 2 generation range from 10-5 (Zn-phthalocyanine) to 4·2 × 10-4 (Ga-phthalocyanine). The efficiency of generating O- 2 was apparently uncorrelated with the phototoxicity of the same dyes. Furthermore, the biological photodamage could not be inhibited by the addition of superoxide dismutase. It is concluded that O- 2 is involved very little, if at all, in the phthalocyanine-induced photo-killing of mammalian cells. © 1985 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
Scientific Publication
You may also be interested in