Co-Authors:
Ben-Hur, E., Department of Radiobiology, Nuclear Research Center-Negev, P.O. Box 9001, Beer-Sheva, Israel
Carmichael, A., Radiation Oncology Branch, Clinical Oncology Program, Division of Cancer Treatment, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20205, United States
Riesz, P., Department of Food Science, A.R.O. Volcani Center, P.O. Box 6, Bet-Dagan, 50250, Israel
Rosenthal, I., Department of Radiobiology, Nuclear Research Center-Negev, P.O. Box 9001, Beer-Sheva, Israel
Abstract:
The effect of the central metal atom on the photodynamic activity of phthalocyanine dyes has been estimated by cytotoxicity to cultured Chinese hamster cells. Chloroaluminium phthalocyanine, followed by the Zn- derivate, were found to be the only active dyes. In parallel it was found that visible light (615 ± 10 nm) excitation of phthalocyanines dissolved in dimethylsulphoxide in the presence of oxygen generates superoxide radical anion. O- 2 radicals were spintrapped with 5,5-dimethyl-1-pyrroline-1-oxide (DMPO) and identified by electron spin resonance. The quantum yields for O- 2 generation range from 10-5 (Zn-phthalocyanine) to 4·2 × 10-4 (Ga-phthalocyanine). The efficiency of generating O- 2 was apparently uncorrelated with the phototoxicity of the same dyes. Furthermore, the biological photodamage could not be inhibited by the addition of superoxide dismutase. It is concluded that O- 2 is involved very little, if at all, in the phthalocyanine-induced photo-killing of mammalian cells. © 1985 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
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