Journal of Immunoassay
Pines, M., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Margel, S., Department of Materials Research, Weizmann Institute of Science, Rehovot, Israel
Polyacrolein (PA) microspheres contain reactive aldehyde groups through which ligands containing primary amino groups such as proteins and drugs can be covalently bound in a single step at physiological pH. Antibodies against cyclic-AMP, digoxin and rabbit serum were thus coupled to PA microspheres. The immuno-microspheres were kept in suspension or freeze-dried, with insignificant decrease in their binding capacity. The conjugates were used in the respective radioimmunoassay (RIA) systems to facilitate the separation of the free and the antibody-bound 125I ligands, in comparison with precipitation of Protein A of Staphylococcus aureus. Cyclic-AMP was assayed using PA microspheres coupled either with the primary antibody or with anti-rabbit serum as a secondary antibody, in a buffer system, in chick plasma, in urine and in media in which avian dispersed kidney cells had been stimulated by various agents. The results obtained using the immuno-microspheres and the bacterial separation methods were indistinguishable. Other 125I-ligands, such as digoxin in buffer system or thyroxine and triiodothyronine in chick plasma, were assayed in the picogram range. Owing to the solubility of non crosslinked microspheres conjugates in toluene-based scintillation fluids, both the free and the bound fractions could be counted when using 3H-ligands. Corticosterone was assayed using this technique. © 1986, Taylor & Francis Group, LLC. All rights reserved.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Polyacrolein microspheres as a new solid phase for radioimmunoassay
7
Pines, M., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Margel, S., Department of Materials Research, Weizmann Institute of Science, Rehovot, Israel
Polyacrolein microspheres as a new solid phase for radioimmunoassay
Polyacrolein (PA) microspheres contain reactive aldehyde groups through which ligands containing primary amino groups such as proteins and drugs can be covalently bound in a single step at physiological pH. Antibodies against cyclic-AMP, digoxin and rabbit serum were thus coupled to PA microspheres. The immuno-microspheres were kept in suspension or freeze-dried, with insignificant decrease in their binding capacity. The conjugates were used in the respective radioimmunoassay (RIA) systems to facilitate the separation of the free and the antibody-bound 125I ligands, in comparison with precipitation of Protein A of Staphylococcus aureus. Cyclic-AMP was assayed using PA microspheres coupled either with the primary antibody or with anti-rabbit serum as a secondary antibody, in a buffer system, in chick plasma, in urine and in media in which avian dispersed kidney cells had been stimulated by various agents. The results obtained using the immuno-microspheres and the bacterial separation methods were indistinguishable. Other 125I-ligands, such as digoxin in buffer system or thyroxine and triiodothyronine in chick plasma, were assayed in the picogram range. Owing to the solubility of non crosslinked microspheres conjugates in toluene-based scintillation fluids, both the free and the bound fractions could be counted when using 3H-ligands. Corticosterone was assayed using this technique. © 1986, Taylor & Francis Group, LLC. All rights reserved.
Scientific Publication