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פותח על ידי קלירמאש פתרונות בע"מ -
Polyacrolein microspheres as a new solid phase for radioimmunoassay
Year:
1986
Source of publication :
Journal of Immunoassay
Authors :
פינס, מרק
;
.
Volume :
7
Co-Authors:
Pines, M., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Margel, S., Department of Materials Research, Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
97
To page:
111
(
Total pages:
15
)
Abstract:
Polyacrolein (PA) microspheres contain reactive aldehyde groups through which ligands containing primary amino groups such as proteins and drugs can be covalently bound in a single step at physiological pH. Antibodies against cyclic-AMP, digoxin and rabbit serum were thus coupled to PA microspheres. The immuno-microspheres were kept in suspension or freeze-dried, with insignificant decrease in their binding capacity. The conjugates were used in the respective radioimmunoassay (RIA) systems to facilitate the separation of the free and the antibody-bound 125I ligands, in comparison with precipitation of Protein A of Staphylococcus aureus. Cyclic-AMP was assayed using PA microspheres coupled either with the primary antibody or with anti-rabbit serum as a secondary antibody, in a buffer system, in chick plasma, in urine and in media in which avian dispersed kidney cells had been stimulated by various agents. The results obtained using the immuno-microspheres and the bacterial separation methods were indistinguishable. Other 125I-ligands, such as digoxin in buffer system or thyroxine and triiodothyronine in chick plasma, were assayed in the picogram range. Owing to the solubility of non crosslinked microspheres conjugates in toluene-based scintillation fluids, both the free and the bound fractions could be counted when using 3H-ligands. Corticosterone was assayed using this technique. © 1986, Taylor & Francis Group, LLC. All rights reserved.
Note:
Related Files :
Adrenal Glands
Animal
Chickens
corticosterone
corticosterone h 3
Cyclic AMP
Digoxin
microsphere
Microspheres
radioimmunoassay
עוד תגיות
תוכן קשור
More details
DOI :
10.1080/01971528608063048
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22773
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:54
Scientific Publication
Polyacrolein microspheres as a new solid phase for radioimmunoassay
7
Pines, M., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Margel, S., Department of Materials Research, Weizmann Institute of Science, Rehovot, Israel
Polyacrolein microspheres as a new solid phase for radioimmunoassay
Polyacrolein (PA) microspheres contain reactive aldehyde groups through which ligands containing primary amino groups such as proteins and drugs can be covalently bound in a single step at physiological pH. Antibodies against cyclic-AMP, digoxin and rabbit serum were thus coupled to PA microspheres. The immuno-microspheres were kept in suspension or freeze-dried, with insignificant decrease in their binding capacity. The conjugates were used in the respective radioimmunoassay (RIA) systems to facilitate the separation of the free and the antibody-bound 125I ligands, in comparison with precipitation of Protein A of Staphylococcus aureus. Cyclic-AMP was assayed using PA microspheres coupled either with the primary antibody or with anti-rabbit serum as a secondary antibody, in a buffer system, in chick plasma, in urine and in media in which avian dispersed kidney cells had been stimulated by various agents. The results obtained using the immuno-microspheres and the bacterial separation methods were indistinguishable. Other 125I-ligands, such as digoxin in buffer system or thyroxine and triiodothyronine in chick plasma, were assayed in the picogram range. Owing to the solubility of non crosslinked microspheres conjugates in toluene-based scintillation fluids, both the free and the bound fractions could be counted when using 3H-ligands. Corticosterone was assayed using this technique. © 1986, Taylor & Francis Group, LLC. All rights reserved.
Scientific Publication
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