Marine Environmental Research
Auslander, M., Israel Oceanographic and Limnological Research, Haifa, Israel
Yudkovski, Y., Israel Oceanographic and Limnological Research, Haifa, Israel
Caspi, V., Ben Gurion University of the Negev, Beer Sheva, Israel
Ophir, R., Weizmann Institute of Science, Rehovot, Israel
Tom, M., Israel Oceanographic and Limnological Research, Haifa, Israel
The demersal fish Lithognathus mormyrus was introduced recently as a sentinel species, detecting biological effects of pollutants present in its natural sandy habitat. Hepatic pollutant-affected transcripts were used as biomarkers. A study aimed at identifying novel pollutant-affected genes, and constructing an operational cDNA microarray used as a multi-gene biomonitoring tool was carried out. The RNA populations were isolated from fish injected with a battery of pollutants, including Aroclor 1254, 17 β-estradiol, CdCl2, perfluoro octanoic acid, Lindane, benzo(a)pyrene, tributyl tin chloride, methylmercury chloride, and 4-nonylphenol. cDNA representations were equalized and they were biased towards enrichment of pollutant-affected genes. This is an abstract of a paper presented at the Symposium "Pollutant Responses in Marine Organisms" (Allesandria, Italy 6/19-23/2005).
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Multi-gene evaluation of hepatic transcript expression in the sentinel fish Lithognathus mormyrus exposed to a battery of pollutants
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Auslander, M., Israel Oceanographic and Limnological Research, Haifa, Israel
Yudkovski, Y., Israel Oceanographic and Limnological Research, Haifa, Israel
Caspi, V., Ben Gurion University of the Negev, Beer Sheva, Israel
Ophir, R., Weizmann Institute of Science, Rehovot, Israel
Tom, M., Israel Oceanographic and Limnological Research, Haifa, Israel
Multi-gene evaluation of hepatic transcript expression in the sentinel fish Lithognathus mormyrus exposed to a battery of pollutants
The demersal fish Lithognathus mormyrus was introduced recently as a sentinel species, detecting biological effects of pollutants present in its natural sandy habitat. Hepatic pollutant-affected transcripts were used as biomarkers. A study aimed at identifying novel pollutant-affected genes, and constructing an operational cDNA microarray used as a multi-gene biomonitoring tool was carried out. The RNA populations were isolated from fish injected with a battery of pollutants, including Aroclor 1254, 17 β-estradiol, CdCl2, perfluoro octanoic acid, Lindane, benzo(a)pyrene, tributyl tin chloride, methylmercury chloride, and 4-nonylphenol. cDNA representations were equalized and they were biased towards enrichment of pollutant-affected genes. This is an abstract of a paper presented at the Symposium "Pollutant Responses in Marine Organisms" (Allesandria, Italy 6/19-23/2005).
Scientific Publication