נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
A new avian fibroblast growth factor receptor in myogenic and chondrogenic cell differentiation
Year:
1994
Source of publication :
Experimental Cell Research
Authors :
מונסונגו-אורנן, אפרת
;
.
פינס, מרק
;
.
Volume :
212
Co-Authors:
Halevy, O., Department of Animal Science, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel, Dept. Biol. Chem. Molec. Pharmacol., Harvard Medical School, Longwood Av. 240, Boston, MA 02115, United States
Monsonego, E., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Marcelle, C., Inst. d'Embryologie, Cell. et Molec., 49 bis, Avenue de la Belle Gabrielle, 94736 Nogent Sur-Marne Cedex, France, Developmental Biology Center, University of California, Irvine, CA 92717, United States
Hodik, V., Department of Animal Science, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Mett, A., Department of Animal Science, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Pines, M., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
278
To page:
284
(
Total pages:
7
)
Abstract:
We studied the expression of FREK (fibroblast growth factor receptor-like embryonic kinase), a new receptor recently cloned from quail embryo, during the differentiation of skeletal muscle satellite cells and epiphyseal growth- plate chondrocytes. Although FREK mRNA was expressed in both cell types, satellite cells expressed higher levels of this mRNA than chondrocytes. FREK gene expression was found to be modulated by b-FGF in a biphasic manner: low concentrations increased expression, whereas high concentrations attenuated it. In both cell cultures, the levels of FREK mRNA declined during terminal differentiation. Moreover, retinoic acid (RA), which induces skeletal muscle satellite cells to differentiate, also caused a reduction in FREK gene expression in these cells. Induction of chondrocyte differentiation with ascorbic acid was monitored by a decrease in collagen type II gene expression and an increase in alkaline phosphatase activity. Satellite cell differentiation was marked by morphological changes as well as by increased sarcomeric myogenin content and creatine kinase activity and changes in the expression of the regulatory muscle-specific genes, MyoD and myogenin. DNA synthesis in both cell types was stimulated by b-FGF. However, in satellite cells, the response was bell-shaped, peaking at 1 ng/ml b-FGF, whereas in chondrocytes, higher levels of b-FGF were needed. b-FGF-dependent DNA synthesis in satellite cells was decreased by RA at concentrations over 10- 7 M. The observed correlation between the level of FREK gene expression and various stages of differentiation, its modulation by b-FGF and RA, as well as the correlation between FREK gene expression and the physiological response to b-FGF, suggest that this specific FGF receptor plays an important role in muscle and cartilage cell differentiation.
Note:
Related Files :
animal cell
Animals
gene expression
Growth plate
Receptor Protein-Tyrosine Kinases
retinoic acid
עוד תגיות
תוכן קשור
More details
DOI :
10.1006/excr.1994.1144
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22799
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:54
You may also be interested in
Scientific Publication
A new avian fibroblast growth factor receptor in myogenic and chondrogenic cell differentiation
212
Halevy, O., Department of Animal Science, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel, Dept. Biol. Chem. Molec. Pharmacol., Harvard Medical School, Longwood Av. 240, Boston, MA 02115, United States
Monsonego, E., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Marcelle, C., Inst. d'Embryologie, Cell. et Molec., 49 bis, Avenue de la Belle Gabrielle, 94736 Nogent Sur-Marne Cedex, France, Developmental Biology Center, University of California, Irvine, CA 92717, United States
Hodik, V., Department of Animal Science, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Mett, A., Department of Animal Science, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Pines, M., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
A new avian fibroblast growth factor receptor in myogenic and chondrogenic cell differentiation
We studied the expression of FREK (fibroblast growth factor receptor-like embryonic kinase), a new receptor recently cloned from quail embryo, during the differentiation of skeletal muscle satellite cells and epiphyseal growth- plate chondrocytes. Although FREK mRNA was expressed in both cell types, satellite cells expressed higher levels of this mRNA than chondrocytes. FREK gene expression was found to be modulated by b-FGF in a biphasic manner: low concentrations increased expression, whereas high concentrations attenuated it. In both cell cultures, the levels of FREK mRNA declined during terminal differentiation. Moreover, retinoic acid (RA), which induces skeletal muscle satellite cells to differentiate, also caused a reduction in FREK gene expression in these cells. Induction of chondrocyte differentiation with ascorbic acid was monitored by a decrease in collagen type II gene expression and an increase in alkaline phosphatase activity. Satellite cell differentiation was marked by morphological changes as well as by increased sarcomeric myogenin content and creatine kinase activity and changes in the expression of the regulatory muscle-specific genes, MyoD and myogenin. DNA synthesis in both cell types was stimulated by b-FGF. However, in satellite cells, the response was bell-shaped, peaking at 1 ng/ml b-FGF, whereas in chondrocytes, higher levels of b-FGF were needed. b-FGF-dependent DNA synthesis in satellite cells was decreased by RA at concentrations over 10- 7 M. The observed correlation between the level of FREK gene expression and various stages of differentiation, its modulation by b-FGF and RA, as well as the correlation between FREK gene expression and the physiological response to b-FGF, suggest that this specific FGF receptor plays an important role in muscle and cartilage cell differentiation.
Scientific Publication
You may also be interested in