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פותח על ידי קלירמאש פתרונות בע"מ -
In vitro infection of host roots by differentiated calli of the parasitic plant Orobanche
Year:
2004
Source of publication :
Journal of Experimental Botany
Authors :
יואל, דניאל
;
.
Volume :
55
Co-Authors:
Zhou, W.J., Institute of Crop Science, Coll. of Agric. and Biotechnology, Zhejiang University, Hangzhou 310029, China, Center for Research on Wild Plants, Utsunomiya University, Utsunomiya 321-8505, Japan
Yoneyama, K., Center for Research on Wild Plants, Utsunomiya University, Utsunomiya 321-8505, Japan
Takeuchi, Y., Center for Research on Wild Plants, Utsunomiya University, Utsunomiya 321-8505, Japan
Iso, S., Center for Research on Wild Plants, Utsunomiya University, Utsunomiya 321-8505, Japan
Rungmekarat, S., Center for Research on Wild Plants, Utsunomiya University, Utsunomiya 321-8505, Japan
Chae, S.H., Center for Research on Wild Plants, Utsunomiya University, Utsunomiya 321-8505, Japan
Sato, D., Center for Research on Wild Plants, Utsunomiya University, Utsunomiya 321-8505, Japan
Joel, D.M., Center for Research on Wild Plants, Utsunomiya University, Utsunomiya 321-8505, Japan, Dept. Phytopathology and Weed Res., ARO, Newe-Ya'ar Research Center, PO Box 1021, Ramat-Yishay, 30095, Israel
Facilitators :
From page:
899
To page:
907
(
Total pages:
9
)
Abstract:
Root parasites of the genus Orobanche are serious weeds in agriculture. An aseptic infection system of host roots using calli of three Orobanche species was developed for the study of host-parasite interaction. The response of calli to various hormonal combinations was studied, because a requirement for infection is the differentiation of root-like protrusions, which are capable of producing haustorial connections to the host. Infectious root-like protrusions develop under the influence of 0.5-1.0 mg l-1 IAA, and under the combination of 0.2 mg l-1 NAA with 5.0 mg l-1 kinetin. These protocols produced root protrusions with pad-like structures that resembled attachment organs of Orobanche seedlings, and proved effective in parasitizing host roots. Direct contact with the medium inhibited haustorium development and prevented infection. To overcome this problem, certain root portions were isolated from the medium by inserting thin glass plates underneath. Calli were then placed on the raised root portions and successfully infected the roots and developed young Orobanche tubercles with vascular system that directly connected to the host.
Note:
Related Files :
biodiversity
Brassica rapa
Brassica rapa subsp. rapa
germination
hormones
Orobanche
proteins
Trifolium
עוד תגיות
תוכן קשור
More details
DOI :
10.1093/jxb/erh098
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22811
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:54
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Scientific Publication
In vitro infection of host roots by differentiated calli of the parasitic plant Orobanche
55
Zhou, W.J., Institute of Crop Science, Coll. of Agric. and Biotechnology, Zhejiang University, Hangzhou 310029, China, Center for Research on Wild Plants, Utsunomiya University, Utsunomiya 321-8505, Japan
Yoneyama, K., Center for Research on Wild Plants, Utsunomiya University, Utsunomiya 321-8505, Japan
Takeuchi, Y., Center for Research on Wild Plants, Utsunomiya University, Utsunomiya 321-8505, Japan
Iso, S., Center for Research on Wild Plants, Utsunomiya University, Utsunomiya 321-8505, Japan
Rungmekarat, S., Center for Research on Wild Plants, Utsunomiya University, Utsunomiya 321-8505, Japan
Chae, S.H., Center for Research on Wild Plants, Utsunomiya University, Utsunomiya 321-8505, Japan
Sato, D., Center for Research on Wild Plants, Utsunomiya University, Utsunomiya 321-8505, Japan
Joel, D.M., Center for Research on Wild Plants, Utsunomiya University, Utsunomiya 321-8505, Japan, Dept. Phytopathology and Weed Res., ARO, Newe-Ya'ar Research Center, PO Box 1021, Ramat-Yishay, 30095, Israel
In vitro infection of host roots by differentiated calli of the parasitic plant Orobanche
Root parasites of the genus Orobanche are serious weeds in agriculture. An aseptic infection system of host roots using calli of three Orobanche species was developed for the study of host-parasite interaction. The response of calli to various hormonal combinations was studied, because a requirement for infection is the differentiation of root-like protrusions, which are capable of producing haustorial connections to the host. Infectious root-like protrusions develop under the influence of 0.5-1.0 mg l-1 IAA, and under the combination of 0.2 mg l-1 NAA with 5.0 mg l-1 kinetin. These protocols produced root protrusions with pad-like structures that resembled attachment organs of Orobanche seedlings, and proved effective in parasitizing host roots. Direct contact with the medium inhibited haustorium development and prevented infection. To overcome this problem, certain root portions were isolated from the medium by inserting thin glass plates underneath. Calli were then placed on the raised root portions and successfully infected the roots and developed young Orobanche tubercles with vascular system that directly connected to the host.
Scientific Publication
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