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The use of the polymerase chain reaction (PCR) for the detection of bean yellow mosaic virus in gladiolus
Year:
1990
Source of publication :
Annals of Applied Biology
Authors :
רוזנר, אריה
;
.
ריגר-שטיין, עדינה
;
.
Volume :
117
Co-Authors:
VUNSH, R., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
ROSNER, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
STEIN, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Facilitators :
From page:
561
To page:
569
(
Total pages:
9
)
Abstract:
A method is described for the improved detection of bean yellow mosaic virus (BYMV) in gladioli leaves. Specific sequences of BYMV RNA, present in total RNA extracts of infected plants were detected following amplification by the polymerase chain reaction (PCR). The viral RNA was initially reverse‐transcribed into cDNA, then specific sequences were amplified by PCR using specific oligonucleotides as primers. Detectable amounts of virus RNA in BYMV‐infected plant tissue by PCR were approximately three to four orders of magnitude lower as compared with those detectable by ELISA and molecular hybridisation. Combining PCR with molecular hybridisation (using a 32P‐labelled transcript of viral sequences as a probe), further increased the sensitivity of this method to a gain of four to five orders of magnitude as compared with direct molecular hybridisation, and enabled the detection of up to single picogram quantities of the virus. Copyright © 1990, Wiley Blackwell. All rights reserved
Note:
Related Files :
bean yellow mosaic potyvirus
Gladiolus
Polymerase Chain Reaction
virus detection
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1744-7348.1990.tb04822.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22824
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:54
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Scientific Publication
The use of the polymerase chain reaction (PCR) for the detection of bean yellow mosaic virus in gladiolus
117
VUNSH, R., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
ROSNER, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
STEIN, A., Department of Virology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
The use of the polymerase chain reaction (PCR) for the detection of bean yellow mosaic virus in gladiolus
A method is described for the improved detection of bean yellow mosaic virus (BYMV) in gladioli leaves. Specific sequences of BYMV RNA, present in total RNA extracts of infected plants were detected following amplification by the polymerase chain reaction (PCR). The viral RNA was initially reverse‐transcribed into cDNA, then specific sequences were amplified by PCR using specific oligonucleotides as primers. Detectable amounts of virus RNA in BYMV‐infected plant tissue by PCR were approximately three to four orders of magnitude lower as compared with those detectable by ELISA and molecular hybridisation. Combining PCR with molecular hybridisation (using a 32P‐labelled transcript of viral sequences as a probe), further increased the sensitivity of this method to a gain of four to five orders of magnitude as compared with direct molecular hybridisation, and enabled the detection of up to single picogram quantities of the virus. Copyright © 1990, Wiley Blackwell. All rights reserved
Scientific Publication
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