נגישות
menu      
חיפוש מתקדם
Annals of Applied Biology
COHEN, J., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
FRANCK, A., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
VETTEN, H.J., Institute of Biochemistry and Plant Virology, Biologische Bundesanstalt Für Land- Und Forstwirtschaft, Braunschweig, Germany
LESEMANN, D.E., Institute of Biochemistry and Plant Virology, Biologische Bundesanstalt Für Land- Und Forstwirtschaft, Braunschweig, Germany
LOEBENSTEIN, G., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
A virus causing sunken veins on ‘Georgia Jet’ sweet potato, and yellow brittle leaves and stunting on Ipomoea setosa, was purified and a specific antiserum was prepared. Flexuous particles with a normal length of 850 nm and a diameter of 12 nm with an open helical structure typical of closteroviruses were observed. The virus particle protein has an apparent mol. wt of c. 34 kD. Double‐stranded RNA isolated from SPSVV‐infected I. setosa and subjected to electrophoresis in agarose consisted of one major band with an estimated Mr of 10.5 kbp and two minor bands with Mr of 9.0 and 5.0 kbp. Fibril‐containing vesicles in phloem cells were observed in ultrathin sections of infected leaf tissues. The virus was transmitted by the whitefly Bemisia tabaci in a semi‐persistent manner and by grafting, but not mechanically. The virus could be transmitted to various Ipomoea species, to Nicotiana clevelandii, N. benthamiana and Amaranthus palmeri. The virus did not react with an antiserum to lettuce infectious yellows virus. Based on particle morphology, serology and symptom expression, the virus appears unique and different from all other reported whitefly‐transmitted closteroviruses. We propose it be named “sweet potato sunken vein virus” (SPSVV). Copyright © 1992, Wiley Blackwell. All rights reserved
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Purification and properties of closterovirus‐like particles associated with a whitefly‐transmitted disease of sweet potato
121
COHEN, J., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
FRANCK, A., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
VETTEN, H.J., Institute of Biochemistry and Plant Virology, Biologische Bundesanstalt Für Land- Und Forstwirtschaft, Braunschweig, Germany
LESEMANN, D.E., Institute of Biochemistry and Plant Virology, Biologische Bundesanstalt Für Land- Und Forstwirtschaft, Braunschweig, Germany
LOEBENSTEIN, G., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Purification and properties of closterovirus‐like particles associated with a whitefly‐transmitted disease of sweet potato
A virus causing sunken veins on ‘Georgia Jet’ sweet potato, and yellow brittle leaves and stunting on Ipomoea setosa, was purified and a specific antiserum was prepared. Flexuous particles with a normal length of 850 nm and a diameter of 12 nm with an open helical structure typical of closteroviruses were observed. The virus particle protein has an apparent mol. wt of c. 34 kD. Double‐stranded RNA isolated from SPSVV‐infected I. setosa and subjected to electrophoresis in agarose consisted of one major band with an estimated Mr of 10.5 kbp and two minor bands with Mr of 9.0 and 5.0 kbp. Fibril‐containing vesicles in phloem cells were observed in ultrathin sections of infected leaf tissues. The virus was transmitted by the whitefly Bemisia tabaci in a semi‐persistent manner and by grafting, but not mechanically. The virus could be transmitted to various Ipomoea species, to Nicotiana clevelandii, N. benthamiana and Amaranthus palmeri. The virus did not react with an antiserum to lettuce infectious yellows virus. Based on particle morphology, serology and symptom expression, the virus appears unique and different from all other reported whitefly‐transmitted closteroviruses. We propose it be named “sweet potato sunken vein virus” (SPSVV). Copyright © 1992, Wiley Blackwell. All rights reserved
Scientific Publication
You may also be interested in