A genomic integration method to visualize localization of endogenous mRNAs in living yeast

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פותח על ידי קלירמאש פתרונות בע"מ -

A genomic integration method to visualize localization of endogenous mRNAs in living yeast

Year: 

2007

Source of publication :

Nature Methods

Authors :

ציפור, גדי

.

Volume :

Co-Authors: 

Haim, L., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Zipor, G., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Aronov, S., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Gerst, J.E., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel

From page: 

409

To page: 

412

(

Total pages: 

4

)

Link :

A genomic integration method to visualize localization of endogenous mRNAs in living yeast

Abstract: 

mRNA localization may be an important determinant for protein localization. We describe a simple PCR-based genomic-tagging strategy (m-TAG) that uses homologous recombination to insert binding sites for the RNA-binding MS2 coat protein (MS2-CP) between the coding region and 3′ untranslated region (UTR) of any yeast gene. Upon coexpression of MS2-CP fused with GFP, we demonstrate the localization of endogenous mRNAs (ASH1, SRO7, PEX3 and OXA1) in living yeast (Saccharomyces cerevisiae).