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פותח על ידי קלירמאש פתרונות בע"מ -
Comparative evaluation and selection of a method for lipid and fatty acid extraction from macroalgae
Year:
2011
Source of publication :
Analytical Biochemistry
Authors :
קומארי, פוג'ה
;
.
Volume :
415
Co-Authors:
Kumari, P., Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), Bhavnagar 364021, Gujarat, India
Reddy, C.R.K., Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), Bhavnagar 364021, Gujarat, India
Jha, B., Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), Bhavnagar 364021, Gujarat, India
Facilitators :
From page:
134
To page:
144
(
Total pages:
11
)
Abstract:
A comparative evaluation of Bligh and Dyer, Folch, and Cequier- Sánchez methods for quantitative determination of total lipids (TLs) and fatty acids (FAs) was accomplished in selective green (Ulva fasciata), red (Gracilaria corticata), and brown algae (Sargassum tenerrimum) using a full factorial categorical design. Applications of sonication and buffer individually on lipid extraction solvent systems were also evaluated. The FA recoveries obtained from the aforementioned methods were compared with those of direct transesterification (DT) methods to identify the best extraction methods. The experimental design showed that macroalgal matrix, extraction method, and buffer were key determinants for TL and FA recoveries (P ≤ 0.05), exhibiting significant interactions. But sonication gave erratic results with no interaction with any of the factors investigated. The buffered solvent system of Folch rendered the highest TL yield in U. fasciata and G. corticata while the buffered system of Bligh and Dyer gave the highest yield in S. tenerrimum. DT methods were more convenient and accurate for FA quantification and rendered 1.5-2 times higher yields when compared with the best conventional method, minimizing the use of chlorinated solvents, their cost of analysis, and disposal. The buffered solvent system was found to be the most appropriate for lipid research in macroalgae. © 2011 Elsevier Inc. All rights reserved.
Note:
Related Files :
Buffers
chemical interaction
fatty acid analysis
fatty acids
Gracilaria corticata
green alga
Lipids
Ulva fasciata
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.ab.2011.04.010
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22941
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:55
Scientific Publication
Comparative evaluation and selection of a method for lipid and fatty acid extraction from macroalgae
415
Kumari, P., Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), Bhavnagar 364021, Gujarat, India
Reddy, C.R.K., Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), Bhavnagar 364021, Gujarat, India
Jha, B., Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), Bhavnagar 364021, Gujarat, India
Comparative evaluation and selection of a method for lipid and fatty acid extraction from macroalgae
A comparative evaluation of Bligh and Dyer, Folch, and Cequier- Sánchez methods for quantitative determination of total lipids (TLs) and fatty acids (FAs) was accomplished in selective green (Ulva fasciata), red (Gracilaria corticata), and brown algae (Sargassum tenerrimum) using a full factorial categorical design. Applications of sonication and buffer individually on lipid extraction solvent systems were also evaluated. The FA recoveries obtained from the aforementioned methods were compared with those of direct transesterification (DT) methods to identify the best extraction methods. The experimental design showed that macroalgal matrix, extraction method, and buffer were key determinants for TL and FA recoveries (P ≤ 0.05), exhibiting significant interactions. But sonication gave erratic results with no interaction with any of the factors investigated. The buffered solvent system of Folch rendered the highest TL yield in U. fasciata and G. corticata while the buffered system of Bligh and Dyer gave the highest yield in S. tenerrimum. DT methods were more convenient and accurate for FA quantification and rendered 1.5-2 times higher yields when compared with the best conventional method, minimizing the use of chlorinated solvents, their cost of analysis, and disposal. The buffered solvent system was found to be the most appropriate for lipid research in macroalgae. © 2011 Elsevier Inc. All rights reserved.
Scientific Publication
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