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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Expression of human serum albumin in the milk of transgenic mice
Year:
1992
Source of publication :
Transgenic Research
Authors :
ברש, איתמר
;
.
דקל, יצחק
;
.
פיירמן, אלכסנדר
;
.
שני, משה
;
.
Volume :
1
Co-Authors:
Shani, M., Institute of Animal Science, ARO, The Volcani Center, Bet-Dagan, 50250, Israel
Barash, I., Institute of Animal Science, ARO, The Volcani Center, Bet-Dagan, 50250, Israel
Nathan, M., Rhône-Poulenc Rorer Central Research, 550 Arcola Road, Collegeville, 19426-0107, PA, United States
Ricca, G., Rhône-Poulenc Rorer Central Research, 550 Arcola Road, Collegeville, 19426-0107, PA, United States
Searfoss, G.H., Rhône-Poulenc Rorer Central Research, 550 Arcola Road, Collegeville, 19426-0107, PA, United States
Dekel, I., Institute of Animal Science, ARO, The Volcani Center, Bet-Dagan, 50250, Israel
Faerman, A., Institute of Animal Science, ARO, The Volcani Center, Bet-Dagan, 50250, Israel
Givol, D., Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot, 76100, Israel
Hurwitz, D.R., Rhône-Poulenc Rorer Central Research, 550 Arcola Road, Collegeville, 19426-0107, PA, United States
Facilitators :
From page:
195
To page:
208
(
Total pages:
14
)
Abstract:
We have tested the feasibility of producing large quantities of human serum albumin (HSA) in the milk of transgenic livestock by generating transgenic mice as a model system. The sheep β-lactoglobulin (BLG) 5′-regulatory promoter sequences were used to support expression of BLG or HSA in transgenic mice. Transgenic animals generated from the entire BLG gene including 3, 5.5 or 10.8 kb of 5′-sequences demonstrated that 3 kb of 5′-sequences were sufficient to support high levels of expression of BLG, and that the longer 5′-sequences did not improve upon the levels of expression. As such, the 3 kb 5′-sequences were used to drive expression of HSA in BLG-HSA constructs. HSA was not detectably expressed in eight transgenic lines generated from a BLG-HSA construct containing the HSA cDNA. Two transgenic lines of 26 generated, using five different constructs, with an HSA minigene possessing the first intron expressed HSA in their milk. One of these expressed HSA at high levels (2.5 mg ml-1) and has stably transmitted this ability to its progeny. A high percentage of transgenic mouse lines (four of six) generated from a vector containing an HSA minigene possessing introns 1 and 2 expressed HSA in their milk at levels which ranged from 1 to 35 μg ml-1. In a similar trend, levels of expression of HSA by transfected tissue culture cells from BLG-HSA vectors containing an introduced SV40 enhancer were low with the HSA cDNA, increased with the HSA minigene with intron 1 and increased further with the minigene containing introns 1 and 2. This study demonstrates that high levels of HSA can be expressed in the milk of transgenic animals, that introns of the HSA gene play a role in its expression and that transfected cell lines may be used to quickly evaluate the relative expression efficiencies of various vector constructs intended for future transgenic evaluation. © 1992 Chapman & Hall.
Note:
Related Files :
Animal
biosynthesis
DNA
gene expression
Genetics
mice
milk
RNA
Serum Albumin
sheep
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF02524750
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22979
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:55
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Scientific Publication
Expression of human serum albumin in the milk of transgenic mice
1
Shani, M., Institute of Animal Science, ARO, The Volcani Center, Bet-Dagan, 50250, Israel
Barash, I., Institute of Animal Science, ARO, The Volcani Center, Bet-Dagan, 50250, Israel
Nathan, M., Rhône-Poulenc Rorer Central Research, 550 Arcola Road, Collegeville, 19426-0107, PA, United States
Ricca, G., Rhône-Poulenc Rorer Central Research, 550 Arcola Road, Collegeville, 19426-0107, PA, United States
Searfoss, G.H., Rhône-Poulenc Rorer Central Research, 550 Arcola Road, Collegeville, 19426-0107, PA, United States
Dekel, I., Institute of Animal Science, ARO, The Volcani Center, Bet-Dagan, 50250, Israel
Faerman, A., Institute of Animal Science, ARO, The Volcani Center, Bet-Dagan, 50250, Israel
Givol, D., Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot, 76100, Israel
Hurwitz, D.R., Rhône-Poulenc Rorer Central Research, 550 Arcola Road, Collegeville, 19426-0107, PA, United States
Expression of human serum albumin in the milk of transgenic mice
We have tested the feasibility of producing large quantities of human serum albumin (HSA) in the milk of transgenic livestock by generating transgenic mice as a model system. The sheep β-lactoglobulin (BLG) 5′-regulatory promoter sequences were used to support expression of BLG or HSA in transgenic mice. Transgenic animals generated from the entire BLG gene including 3, 5.5 or 10.8 kb of 5′-sequences demonstrated that 3 kb of 5′-sequences were sufficient to support high levels of expression of BLG, and that the longer 5′-sequences did not improve upon the levels of expression. As such, the 3 kb 5′-sequences were used to drive expression of HSA in BLG-HSA constructs. HSA was not detectably expressed in eight transgenic lines generated from a BLG-HSA construct containing the HSA cDNA. Two transgenic lines of 26 generated, using five different constructs, with an HSA minigene possessing the first intron expressed HSA in their milk. One of these expressed HSA at high levels (2.5 mg ml-1) and has stably transmitted this ability to its progeny. A high percentage of transgenic mouse lines (four of six) generated from a vector containing an HSA minigene possessing introns 1 and 2 expressed HSA in their milk at levels which ranged from 1 to 35 μg ml-1. In a similar trend, levels of expression of HSA by transfected tissue culture cells from BLG-HSA vectors containing an introduced SV40 enhancer were low with the HSA cDNA, increased with the HSA minigene with intron 1 and increased further with the minigene containing introns 1 and 2. This study demonstrates that high levels of HSA can be expressed in the milk of transgenic animals, that introns of the HSA gene play a role in its expression and that transfected cell lines may be used to quickly evaluate the relative expression efficiencies of various vector constructs intended for future transgenic evaluation. © 1992 Chapman & Hall.
Scientific Publication
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