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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Identification and characterization of Phoma tracheiphila mutants impaired in pathogenicity following Agrobacterium-mediated mutagenesis
Year:
2013
Source of publication :
Phytoparasitica
Authors :
בלאוסוב, אדוארד
;
.
גת, תמי
;
.
ליארזי, אורנה
;
.
סקובורודניקובה, יוליה
;
.
עזרא, דוד
;
.
קרויטור, תמי
;
.
Volume :
41
Co-Authors:
Kroitor-Keren, T., Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Liarzi, O., Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Gat, T., Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Skovorodnikova, J., Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Belausov, E., Plant Sciences Institute, Ornamental Horticulture, Microscopy Unit, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Ezra, D., Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
491
To page:
502
(
Total pages:
12
)
Abstract:
Mal secco disease, caused by the pathogenic fungus Phoma tracheiphila, is a devastating disease of susceptible citrus species, especially lemon. To study the molecular interactions between the pathogen and its host, a method for identifying the genes involved in pathogenicity is needed. This work describes the transformation of P. tracheiphila phialoconidia by Agrobacterium tumefaciens, and the generation of mutated P. tracheiphila isolates exhibiting reduced virulence on rough lemon seedlings. A rapid, replicable, and reliable method for screening P. tracheiphila mutants to assess their virulence by using rough lemon seedlings was developed. Among 2263 transformants obtained, three were non-virulent and 43 displayed reduced virulence. In addition, one of the transformants, which exhibited virulence similar to that of the wild type, was used for in planta visualization of the fungus progression through the plant xylem. To our knowledge, this is the first report of A. tumefaciens-mediated transformation of P. tracheiphila, and subsequent screening of the transformants to identify non-virulent mutants. © 2013 Springer Science+Business Media Dordrecht.
Note:
Related Files :
Agrobacterium
Citrus limon
fungi
RNA-directed RNA polymerase 2
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s12600-013-0328-7
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23008
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:56
Scientific Publication
Identification and characterization of Phoma tracheiphila mutants impaired in pathogenicity following Agrobacterium-mediated mutagenesis
41
Kroitor-Keren, T., Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Liarzi, O., Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Gat, T., Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Skovorodnikova, J., Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Belausov, E., Plant Sciences Institute, Ornamental Horticulture, Microscopy Unit, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Ezra, D., Department of Plant Pathology, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Identification and characterization of Phoma tracheiphila mutants impaired in pathogenicity following Agrobacterium-mediated mutagenesis
Mal secco disease, caused by the pathogenic fungus Phoma tracheiphila, is a devastating disease of susceptible citrus species, especially lemon. To study the molecular interactions between the pathogen and its host, a method for identifying the genes involved in pathogenicity is needed. This work describes the transformation of P. tracheiphila phialoconidia by Agrobacterium tumefaciens, and the generation of mutated P. tracheiphila isolates exhibiting reduced virulence on rough lemon seedlings. A rapid, replicable, and reliable method for screening P. tracheiphila mutants to assess their virulence by using rough lemon seedlings was developed. Among 2263 transformants obtained, three were non-virulent and 43 displayed reduced virulence. In addition, one of the transformants, which exhibited virulence similar to that of the wild type, was used for in planta visualization of the fungus progression through the plant xylem. To our knowledge, this is the first report of A. tumefaciens-mediated transformation of P. tracheiphila, and subsequent screening of the transformants to identify non-virulent mutants. © 2013 Springer Science+Business Media Dordrecht.
Scientific Publication
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