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פותח על ידי קלירמאש פתרונות בע"מ -
Mitochondrion-mediated apoptosis is enhanced in long-lived αMUPA transgenic mice and calorically restricted wild-type mice
Year:
2003
Source of publication :
Experimental Gerontology
Authors :
יהב, שלמה
;
.
Volume :
38
Co-Authors:
Tirosh, O., Institute of Biochemistry, Food Science and Nutrition, Hebrew University of Jerusalem, Rehovot 76100, Israel
Aronis, A., Institute of Biochemistry, Food Science and Nutrition, Hebrew University of Jerusalem, Rehovot 76100, Israel
Zusman, I., Koret School of Veterinary Medicine, Hebrew University of Jerusalem, Rehovot 76100, Israel
Kossoy, G., Koret School of Veterinary Medicine, Hebrew University of Jerusalem, Rehovot 76100, Israel
Yahav, S., Institute of Animal Science, Agricultural Research Organization, Bet Dagan, Israel
Shinder, D.
Abramovitz, R., Department of Biological Chemistry, Weizmann Institute of Science, P.O. Box 26, Rehovot 76100, Israel
Miskin, R., Department of Biological Chemistry, Weizmann Institute of Science, P.O. Box 26, Rehovot 76100, Israel
Facilitators :
From page:
955
To page:
963
(
Total pages:
9
)
Abstract:
Caloric restriction (CR) can extend the life-span of multiple species and is the only intervention known to attenuate aging in mammals. Mechanisms mediating the CR influence are as yet unclear. To get insight into these mechanisms we took advantage of αMUPA transgenic mice that have previously been reported to spontaneously eat less and live longer compared with their wild-type (WT) control. Here we report that mitochondria isolated from young adult αMUPA livers showed increased susceptibility to calcium-induced high-amplitude swelling, increased cytochrome c release and enhanced glutathione levels. Furthermore, young adult αMUPA mice showed significantly enhanced caspase-3 activity in liver homogenates, increased fraction of apoptotic hepatocytes, and a lower level of serum IGF-1. In addition, αMUPA mice showed a decreased rate of spontaneously occurring lung tumors at an old age. Short-term (8 weeks) calorically restricted WT mice also showed an increase of mitochondrial swelling and caspase-3 activity compared with ad libitum (AL) fed WT mice. These results provide the first indication that CR can enhance mitochondrion-mediated apoptotic capacity. Collectively, the results are consistent with the possibility that long lasting, moderately increased apoptotic capacity, possibly linked in part to IGF-1 and GSH modulation, could play a role in the CR-induced anti-aging influence in mice. © 2003 Published by Elsevier Inc.
Note:
Related Files :
aging
Animals
animal tissue
apoptosis
cytochrome c
Female
Insulin-Like Growth Factor I
lung tumor
Mammalia
mice
senescence
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/S0531-5565(03)00151-7
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23019
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:56
Scientific Publication
Mitochondrion-mediated apoptosis is enhanced in long-lived αMUPA transgenic mice and calorically restricted wild-type mice
38
Tirosh, O., Institute of Biochemistry, Food Science and Nutrition, Hebrew University of Jerusalem, Rehovot 76100, Israel
Aronis, A., Institute of Biochemistry, Food Science and Nutrition, Hebrew University of Jerusalem, Rehovot 76100, Israel
Zusman, I., Koret School of Veterinary Medicine, Hebrew University of Jerusalem, Rehovot 76100, Israel
Kossoy, G., Koret School of Veterinary Medicine, Hebrew University of Jerusalem, Rehovot 76100, Israel
Yahav, S., Institute of Animal Science, Agricultural Research Organization, Bet Dagan, Israel
Shinder, D.
Abramovitz, R., Department of Biological Chemistry, Weizmann Institute of Science, P.O. Box 26, Rehovot 76100, Israel
Miskin, R., Department of Biological Chemistry, Weizmann Institute of Science, P.O. Box 26, Rehovot 76100, Israel
Mitochondrion-mediated apoptosis is enhanced in long-lived αMUPA transgenic mice and calorically restricted wild-type mice
Caloric restriction (CR) can extend the life-span of multiple species and is the only intervention known to attenuate aging in mammals. Mechanisms mediating the CR influence are as yet unclear. To get insight into these mechanisms we took advantage of αMUPA transgenic mice that have previously been reported to spontaneously eat less and live longer compared with their wild-type (WT) control. Here we report that mitochondria isolated from young adult αMUPA livers showed increased susceptibility to calcium-induced high-amplitude swelling, increased cytochrome c release and enhanced glutathione levels. Furthermore, young adult αMUPA mice showed significantly enhanced caspase-3 activity in liver homogenates, increased fraction of apoptotic hepatocytes, and a lower level of serum IGF-1. In addition, αMUPA mice showed a decreased rate of spontaneously occurring lung tumors at an old age. Short-term (8 weeks) calorically restricted WT mice also showed an increase of mitochondrial swelling and caspase-3 activity compared with ad libitum (AL) fed WT mice. These results provide the first indication that CR can enhance mitochondrion-mediated apoptotic capacity. Collectively, the results are consistent with the possibility that long lasting, moderately increased apoptotic capacity, possibly linked in part to IGF-1 and GSH modulation, could play a role in the CR-induced anti-aging influence in mice. © 2003 Published by Elsevier Inc.
Scientific Publication
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