Abstract:
Circularly polarized chlorophyll luminescence (CPL) was recently shown to be an effective tool for the study of chiral macroaggregate formation of the light-harvesting chlorophyll a/b pigment-protein complexes (LHCIIs) in isolated chloroplasts. The CPL measuring system was modified to study green leaves. Spectral and intensity features of pea leaf CPL signals suggested that CPL indeed detected chiral macroaggregates. The signals were found to depend on the excitation vs emission optical alignment, as well as on the side (adaxial or abaxial) of the leaf. Illumination of attached leaves with either low intensity or strong photoinhibitory light did not affect the chiral macroaggregation status. In contrast, the induction of drought stress in detached leaves led to full disruption of the chiral macroaggregates. The disruption developed gradually during slow dehydration, whereas under fast, heat-stimulated dehydration it was manifested in cooperative diminution of both CPL and photochemical capacity. Simultaneous exposure of the leaves to photoinhibitory illumination and fast dehydration resulted in negative CPL signals. The results demonstrate the potency of CPL as a non-destructive tool for structural studies of LHCII in intact leaves under varying environmental conditions.
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מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
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