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פותח על ידי קלירמאש פתרונות בע"מ -
CaAP2 transcription factor is a candidate gene for a flowering repressor and a candidate for controlling natural variation of flowering time in Capsicum annuum
Year:
2015
Source of publication :
Theoretical and Applied Genetics
Authors :
בורובסקי, ילנה
;
.
פארן, אילן
;
.
Volume :
128
Co-Authors:
Borovsky, Y., Institute of Plant Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, Israel
Sharma, V.K., Institute of Plant Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, Israel, Indian Agricultural Research Institute, Regional Station Katrain, Kullu Valley, HP, India
Verbakel, H., Nunhems Netherlands BV, Bayer CropScience Vegetable Seeds, PO Box 4005, Haelen, Netherlands
Paran, I., Institute of Plant Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, Israel
Facilitators :
From page:
1073
To page:
1082
(
Total pages:
10
)
Abstract:
Key message: TheAPETALA2transcription factor homologCaAP2is a candidate gene for a flowering repressor in pepper, as revealed by induced-mutation phenotype, and a candidate underlying a major QTL controlling natural variation in flowering time. Abstract: To decipher the genetic control of transition to flowering in pepper (Capsicum spp.) and determine the extent of gene function conservation compared to model species, we isolated and characterized several ethyl methanesulfonate (EMS)-induced mutants that vary in their flowering time compared to the wild type. In the present study, we report on the isolation of an early-flowering mutant that flowers after four leaves on the primary stem compared to nine leaves in the wild-type ‘Maor’. By genetic mapping and sequencing of putative candidate genes linked to the mutant phenotype, we identified a member of the APETALA2 (AP2) transcription factor family, CaAP2, which was disrupted in the early-flowering mutant. CaAP2 is a likely ortholog of AP2 that functions as a repressor of flowering in Arabidopsis. To test whether CaAP2 has an effect on controlling natural variation in the transition to flowering in pepper, we performed QTL mapping for flowering time in a cross between early and late-flowering C. annuum accessions. We identified a major QTL in a region of chromosome 2 in which CaAP2 was the most significant marker, explaining 52 % of the phenotypic variation of the trait. Sequence comparison of the CaAP2 open reading frames in the two parents used for QTL mapping did not reveal significant variation. In contrast, significant differences in expression level of CaAP2 were detected between near-isogenic lines that differ for the flowering time QTL, supporting the putative function of CaAP2 as a major repressor of flowering in pepper. © 2015, Springer-Verlag Berlin Heidelberg.
Note:
Related Files :
chromosome map
chromosome mapping
Genes
Genetics
metabolism
phenotype
Putative functions
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00122-015-2491-3
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23049
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:56
Scientific Publication
CaAP2 transcription factor is a candidate gene for a flowering repressor and a candidate for controlling natural variation of flowering time in Capsicum annuum
128
Borovsky, Y., Institute of Plant Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, Israel
Sharma, V.K., Institute of Plant Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, Israel, Indian Agricultural Research Institute, Regional Station Katrain, Kullu Valley, HP, India
Verbakel, H., Nunhems Netherlands BV, Bayer CropScience Vegetable Seeds, PO Box 4005, Haelen, Netherlands
Paran, I., Institute of Plant Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, Israel
CaAP2 transcription factor is a candidate gene for a flowering repressor and a candidate for controlling natural variation of flowering time in Capsicum annuum
Key message: TheAPETALA2transcription factor homologCaAP2is a candidate gene for a flowering repressor in pepper, as revealed by induced-mutation phenotype, and a candidate underlying a major QTL controlling natural variation in flowering time. Abstract: To decipher the genetic control of transition to flowering in pepper (Capsicum spp.) and determine the extent of gene function conservation compared to model species, we isolated and characterized several ethyl methanesulfonate (EMS)-induced mutants that vary in their flowering time compared to the wild type. In the present study, we report on the isolation of an early-flowering mutant that flowers after four leaves on the primary stem compared to nine leaves in the wild-type ‘Maor’. By genetic mapping and sequencing of putative candidate genes linked to the mutant phenotype, we identified a member of the APETALA2 (AP2) transcription factor family, CaAP2, which was disrupted in the early-flowering mutant. CaAP2 is a likely ortholog of AP2 that functions as a repressor of flowering in Arabidopsis. To test whether CaAP2 has an effect on controlling natural variation in the transition to flowering in pepper, we performed QTL mapping for flowering time in a cross between early and late-flowering C. annuum accessions. We identified a major QTL in a region of chromosome 2 in which CaAP2 was the most significant marker, explaining 52 % of the phenotypic variation of the trait. Sequence comparison of the CaAP2 open reading frames in the two parents used for QTL mapping did not reveal significant variation. In contrast, significant differences in expression level of CaAP2 were detected between near-isogenic lines that differ for the flowering time QTL, supporting the putative function of CaAP2 as a major repressor of flowering in pepper. © 2015, Springer-Verlag Berlin Heidelberg.
Scientific Publication
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