נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Immunochemical and biological analysis of pheromone biosynthesis activating neuropeptide in Heliothis peltigera
Year:
1992
Authors :
אלטשטיין, מרים
;
.
בן-עזיז, אורנה
;
.
גזית, יואב
;
.
דונקלבלום, עזרא
;
.
Volume :
19
Co-Authors:
Gazit, Y., Institute of Plant Protection, ARO, The Volcani Center, Bet Dagan, Israel
Dunkelblum, E., Institute of Plant Protection, ARO, The Volcani Center, Bet Dagan, Israel
Ben‐Aziz, O., Institute of Plant Protection, ARO, The Volcani Center, Bet Dagan, Israel
Altstein, M., Institute of Plant Protection, ARO, The Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
247
To page:
260
(
Total pages:
14
)
Abstract:
This study describes the preparation and characterization of a highly specific antiserum to Helicoverpa zea pheromone biosynthesis activating neuropeptide (Hez‐PBAN), and the use of thsi antiserum, in an enzyme linked immunosorbent assay (ELISA), to determine: (a) the content of endogenous PBAN in head extracts of male and female Heliothis peltigera; (b) the level of PBAN at different developmental stages; and (c) the content of PBAN in four different moth species. Cross‐reactivity studies revealed that the antiserum is directed mainly toward the N‐terminal region of the neuropeptide, and that it exhibits similar binding affinities toward the oxidized and reduced forms of PBAN. Analysis of PBAN content in head extracts of male and female H. peltigera, at scotophase, revealed the presence of 4.97 and 4.58 pmol, respectively, in 3‐day‐old moths, and 5.33 and 4.78 pmol, respectively, in 7‐day‐old moths. The similarity in the content of PBAN at both ages and sexes was in accordance with the amount of pheromonotropic activity in these extracts which stimulated pheromone biosynthesis to a similar level. Analysis of PBAN‐like immunoreactivity (IR) in head extracts of H. peltigera larvae and pupae demonstrated the existence of the neuropeptide in the 4th larval instar and continued to increase as a function of development. No IR could be detected in the first three larval instars. The larval and pupal extracts also exerted pheromonotropic activity which followed a similar pattern. The activity in these extracts, however, was considerably lower than that found in adult male and female heads. IR was also detected in head extracts of three other Noctuidae moths: Helicoverpa armigera, Cornutiplusia circumflexa and Spodoptera littoralis, indicating a high degree of chemcial and structural similarity of PBAN in these moths. © 1992 Wiley‐Liss, Inc. Copyright © 1992 Wiley‐Liss, Inc.
Note:
Related Files :
Animal
Female
Growth, Development and Aging
Male
metabolism
neuropeptide
עוד תגיות
תוכן קשור
More details
DOI :
10.1002/arch.940190404
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23054
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:56
You may also be interested in
Scientific Publication
Immunochemical and biological analysis of pheromone biosynthesis activating neuropeptide in Heliothis peltigera
19
Gazit, Y., Institute of Plant Protection, ARO, The Volcani Center, Bet Dagan, Israel
Dunkelblum, E., Institute of Plant Protection, ARO, The Volcani Center, Bet Dagan, Israel
Ben‐Aziz, O., Institute of Plant Protection, ARO, The Volcani Center, Bet Dagan, Israel
Altstein, M., Institute of Plant Protection, ARO, The Volcani Center, Bet Dagan, Israel
Immunochemical and biological analysis of pheromone biosynthesis activating neuropeptide in Heliothis peltigera
This study describes the preparation and characterization of a highly specific antiserum to Helicoverpa zea pheromone biosynthesis activating neuropeptide (Hez‐PBAN), and the use of thsi antiserum, in an enzyme linked immunosorbent assay (ELISA), to determine: (a) the content of endogenous PBAN in head extracts of male and female Heliothis peltigera; (b) the level of PBAN at different developmental stages; and (c) the content of PBAN in four different moth species. Cross‐reactivity studies revealed that the antiserum is directed mainly toward the N‐terminal region of the neuropeptide, and that it exhibits similar binding affinities toward the oxidized and reduced forms of PBAN. Analysis of PBAN content in head extracts of male and female H. peltigera, at scotophase, revealed the presence of 4.97 and 4.58 pmol, respectively, in 3‐day‐old moths, and 5.33 and 4.78 pmol, respectively, in 7‐day‐old moths. The similarity in the content of PBAN at both ages and sexes was in accordance with the amount of pheromonotropic activity in these extracts which stimulated pheromone biosynthesis to a similar level. Analysis of PBAN‐like immunoreactivity (IR) in head extracts of H. peltigera larvae and pupae demonstrated the existence of the neuropeptide in the 4th larval instar and continued to increase as a function of development. No IR could be detected in the first three larval instars. The larval and pupal extracts also exerted pheromonotropic activity which followed a similar pattern. The activity in these extracts, however, was considerably lower than that found in adult male and female heads. IR was also detected in head extracts of three other Noctuidae moths: Helicoverpa armigera, Cornutiplusia circumflexa and Spodoptera littoralis, indicating a high degree of chemcial and structural similarity of PBAN in these moths. © 1992 Wiley‐Liss, Inc. Copyright © 1992 Wiley‐Liss, Inc.
Scientific Publication
You may also be interested in