חיפוש מתקדם
Kapat, A., Department of Plant Pathology, A.R.O, Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Zimand, G., Department of Plant Pathology, A.R.O, Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Elad, Y., Department of Plant Pathology, A.R.O, Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Two isolates of Trichoderma harzianum (T39 a biocontrol agent, and NCIM 1185) were tested for their capacity to reduce the level of hydrolytic enzymes produced by Botrytis cinerea both in vitro and in vivo, and to inhibit infection caused by B. cinerea conidia. The hydrolytic enzymes studied were cutin esterase, polygalacturonases (PG), pectin methyl esterase (PME), pectate lyase (PL) and carboxymethyl cellulase (CMCase). On the surface of bean (Phaseolus vulgaris) leaves, isolate T39 inhibited germination of B. cinerea conidia and subsequent disease development significantly better than isolate NCIM1185. The levels of the various hydrolytic enzymes were reduced when B. cinerea was grown either with a T. harzianum isolate in liquid culture or on the surface of bean leaves. T39 reduced enzyme levels by about 64, 80, 25, 77, 42 and 23% for cutin esterase, exoPG, endoPG, PME, PL and CMCase, respectively. NCIM1185 caused 57, 60, 5, 87 and 11% reduction in the production of cutin esterase, exoPG, endoPG, PME, PL and CMCase, respectively. However, NCIM1185 enhanced the production of PL when it was grown with B. cinerea in liquid culture medium. On the surface of bean leaves, T39 was the better inhibitory agent. The only exception was CMCase, the presence of which was not inhibited by either isolate. Cutin esterase, exo and endoPG, PME and PL of B. cinerea on bean leaves were reduced in the presence of T39 by ca 20, 95, 100-50, 100 and 95%, respectively. The importance of reduced enzyme activity of B. cinerea in the biocontrol activity of T. harzianum is discussed.
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הספר "אוצר וולקני"
אודות
תנאי שימוש
Effect of two isolates of Trichoderma harzianum on the activity of hydrolytic enzymes produced by Botrytis cinerea
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Kapat, A., Department of Plant Pathology, A.R.O, Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Zimand, G., Department of Plant Pathology, A.R.O, Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Elad, Y., Department of Plant Pathology, A.R.O, Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Effect of two isolates of Trichoderma harzianum on the activity of hydrolytic enzymes produced by Botrytis cinerea
Two isolates of Trichoderma harzianum (T39 a biocontrol agent, and NCIM 1185) were tested for their capacity to reduce the level of hydrolytic enzymes produced by Botrytis cinerea both in vitro and in vivo, and to inhibit infection caused by B. cinerea conidia. The hydrolytic enzymes studied were cutin esterase, polygalacturonases (PG), pectin methyl esterase (PME), pectate lyase (PL) and carboxymethyl cellulase (CMCase). On the surface of bean (Phaseolus vulgaris) leaves, isolate T39 inhibited germination of B. cinerea conidia and subsequent disease development significantly better than isolate NCIM1185. The levels of the various hydrolytic enzymes were reduced when B. cinerea was grown either with a T. harzianum isolate in liquid culture or on the surface of bean leaves. T39 reduced enzyme levels by about 64, 80, 25, 77, 42 and 23% for cutin esterase, exoPG, endoPG, PME, PL and CMCase, respectively. NCIM1185 caused 57, 60, 5, 87 and 11% reduction in the production of cutin esterase, exoPG, endoPG, PME, PL and CMCase, respectively. However, NCIM1185 enhanced the production of PL when it was grown with B. cinerea in liquid culture medium. On the surface of bean leaves, T39 was the better inhibitory agent. The only exception was CMCase, the presence of which was not inhibited by either isolate. Cutin esterase, exo and endoPG, PME and PL of B. cinerea on bean leaves were reduced in the presence of T39 by ca 20, 95, 100-50, 100 and 95%, respectively. The importance of reduced enzyme activity of B. cinerea in the biocontrol activity of T. harzianum is discussed.
Scientific Publication
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