נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Effect of two isolates of Trichoderma harzianum on the activity of hydrolytic enzymes produced by Botrytis cinerea
Year:
1998
Authors :
אלעד, יגאל
;
.
צימנד, גילי
;
.
Volume :
52
Co-Authors:
Kapat, A., Department of Plant Pathology, A.R.O, Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Zimand, G., Department of Plant Pathology, A.R.O, Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Elad, Y., Department of Plant Pathology, A.R.O, Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Facilitators :
From page:
127
To page:
137
(
Total pages:
11
)
Abstract:
Two isolates of Trichoderma harzianum (T39 a biocontrol agent, and NCIM 1185) were tested for their capacity to reduce the level of hydrolytic enzymes produced by Botrytis cinerea both in vitro and in vivo, and to inhibit infection caused by B. cinerea conidia. The hydrolytic enzymes studied were cutin esterase, polygalacturonases (PG), pectin methyl esterase (PME), pectate lyase (PL) and carboxymethyl cellulase (CMCase). On the surface of bean (Phaseolus vulgaris) leaves, isolate T39 inhibited germination of B. cinerea conidia and subsequent disease development significantly better than isolate NCIM1185. The levels of the various hydrolytic enzymes were reduced when B. cinerea was grown either with a T. harzianum isolate in liquid culture or on the surface of bean leaves. T39 reduced enzyme levels by about 64, 80, 25, 77, 42 and 23% for cutin esterase, exoPG, endoPG, PME, PL and CMCase, respectively. NCIM1185 caused 57, 60, 5, 87 and 11% reduction in the production of cutin esterase, exoPG, endoPG, PME, PL and CMCase, respectively. However, NCIM1185 enhanced the production of PL when it was grown with B. cinerea in liquid culture medium. On the surface of bean leaves, T39 was the better inhibitory agent. The only exception was CMCase, the presence of which was not inhibited by either isolate. Cutin esterase, exo and endoPG, PME and PL of B. cinerea on bean leaves were reduced in the presence of T39 by ca 20, 95, 100-50, 100 and 95%, respectively. The importance of reduced enzyme activity of B. cinerea in the biocontrol activity of T. harzianum is discussed.
Note:
Related Files :
germination
hydrolase
liquid culture
pectate lyase
Phaseolus vulgaris
Polygalacturonase
עוד תגיות
תוכן קשור
More details
DOI :
10.1006/pmpp.1997.0140
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23135
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:57
Scientific Publication
Effect of two isolates of Trichoderma harzianum on the activity of hydrolytic enzymes produced by Botrytis cinerea
52
Kapat, A., Department of Plant Pathology, A.R.O, Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Zimand, G., Department of Plant Pathology, A.R.O, Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Elad, Y., Department of Plant Pathology, A.R.O, Volcani Center, P.O.B. 6, Bet Dagan 50250, Israel
Effect of two isolates of Trichoderma harzianum on the activity of hydrolytic enzymes produced by Botrytis cinerea
Two isolates of Trichoderma harzianum (T39 a biocontrol agent, and NCIM 1185) were tested for their capacity to reduce the level of hydrolytic enzymes produced by Botrytis cinerea both in vitro and in vivo, and to inhibit infection caused by B. cinerea conidia. The hydrolytic enzymes studied were cutin esterase, polygalacturonases (PG), pectin methyl esterase (PME), pectate lyase (PL) and carboxymethyl cellulase (CMCase). On the surface of bean (Phaseolus vulgaris) leaves, isolate T39 inhibited germination of B. cinerea conidia and subsequent disease development significantly better than isolate NCIM1185. The levels of the various hydrolytic enzymes were reduced when B. cinerea was grown either with a T. harzianum isolate in liquid culture or on the surface of bean leaves. T39 reduced enzyme levels by about 64, 80, 25, 77, 42 and 23% for cutin esterase, exoPG, endoPG, PME, PL and CMCase, respectively. NCIM1185 caused 57, 60, 5, 87 and 11% reduction in the production of cutin esterase, exoPG, endoPG, PME, PL and CMCase, respectively. However, NCIM1185 enhanced the production of PL when it was grown with B. cinerea in liquid culture medium. On the surface of bean leaves, T39 was the better inhibitory agent. The only exception was CMCase, the presence of which was not inhibited by either isolate. Cutin esterase, exo and endoPG, PME and PL of B. cinerea on bean leaves were reduced in the presence of T39 by ca 20, 95, 100-50, 100 and 95%, respectively. The importance of reduced enzyme activity of B. cinerea in the biocontrol activity of T. harzianum is discussed.
Scientific Publication
You may also be interested in