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פותח על ידי קלירמאש פתרונות בע"מ -
Concomitant Induction of Systemic Resistance to Pseudomonas syringae pv. lachrymans in Cucumber by Trichoderma asperellum (T-203) and Accumulation of Phytoalexins
Year:
2003
Authors :
ידידיה, איריס
;
.
קפולניק, יורם
;
.
שורש, מיכל
;
.
Volume :
69
Co-Authors:
Yedidia, I., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Shoresh, M., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Kerem, Z., Institute of Biochemistry, Fac. Agric., Food/Environ. Qual. S., Hebrew University of Jerusalem, Rehovot 76100, Israel
Benhamou, N., Rech. en Sci. de la Vie et de la S., Pavillion Charles-Eugene-Marchand, Laval University, University City, Que. G1K 7P4, Canada
Kapulnik, Y., Dept. of Agronomy and Nat. Resources, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Chet, I., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Facilitators :
From page:
7343
To page:
7353
(
Total pages:
11
)
Abstract:
Most studies on the reduction of disease incidence in soil treated with Trichoderma asperellum have focused on microbial interactions rather than on plant responses. This study presents conclusive evidence for the induction of a systemic response against angular leaf spot of cucumber (Pseudomonas syringae pv. lachrymans) following application of T. asperellum to the root system. To ascertain that T. asperellum was the only micro. organism present in the root milieu, plants were grown in an aseptic hydroponic growth system. Disease symptoms were reduced by as much as 80%, corresponding to a reduction of 2 orders of magnitude in bacterial cell densities in leaves of plants pretreated with T. asperellum. As revealed by electron microscopy, bacterial cell proliferation in these plants was halted. The protection afforded by the biocontrol agent was associated with the accumulation of mRNA of two defense genes: the phenylpropanoid pathway gene encoding phenylalanine ammonia lyase (PAL) and the lipoxygenase pathway gene encoding hydroxyperoxide lyase (HPL). This was further supported by the accumulation of secondary metabolites of a phenolic nature that showed an increase of up to sixfold in inhibition capacity of bacterial growth in vitro. The bulk of the antimicrobial activity was found in the acid-hydrolyzed extract containing the phenolics in their aglycone form. High-performance liquid chromatography analysis of phenolic compounds showed a marked change in their profile in the challenged, preelicited plants relative to that in challenged controls. The results suggest that similar to beneficial rhizobacteria, T. asperellum may activate separate metabolic pathways in cucumber that are involved in plant signaling and biosynthesis, eventually leading to the systemic accumulation of phytoalexins.
Note:
Related Files :
bacteria
biosynthesis
Cucumis sativus
enzymes
Genetics
hydroponics
Trichoderma
עוד תגיות
תוכן קשור
More details
DOI :
10.1128/AEM.69.12.7343-7353.2003
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23151
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:57
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Scientific Publication
Concomitant Induction of Systemic Resistance to Pseudomonas syringae pv. lachrymans in Cucumber by Trichoderma asperellum (T-203) and Accumulation of Phytoalexins
69
Yedidia, I., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Shoresh, M., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Kerem, Z., Institute of Biochemistry, Fac. Agric., Food/Environ. Qual. S., Hebrew University of Jerusalem, Rehovot 76100, Israel
Benhamou, N., Rech. en Sci. de la Vie et de la S., Pavillion Charles-Eugene-Marchand, Laval University, University City, Que. G1K 7P4, Canada
Kapulnik, Y., Dept. of Agronomy and Nat. Resources, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Chet, I., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Concomitant Induction of Systemic Resistance to Pseudomonas syringae pv. lachrymans in Cucumber by Trichoderma asperellum (T-203) and Accumulation of Phytoalexins
Most studies on the reduction of disease incidence in soil treated with Trichoderma asperellum have focused on microbial interactions rather than on plant responses. This study presents conclusive evidence for the induction of a systemic response against angular leaf spot of cucumber (Pseudomonas syringae pv. lachrymans) following application of T. asperellum to the root system. To ascertain that T. asperellum was the only micro. organism present in the root milieu, plants were grown in an aseptic hydroponic growth system. Disease symptoms were reduced by as much as 80%, corresponding to a reduction of 2 orders of magnitude in bacterial cell densities in leaves of plants pretreated with T. asperellum. As revealed by electron microscopy, bacterial cell proliferation in these plants was halted. The protection afforded by the biocontrol agent was associated with the accumulation of mRNA of two defense genes: the phenylpropanoid pathway gene encoding phenylalanine ammonia lyase (PAL) and the lipoxygenase pathway gene encoding hydroxyperoxide lyase (HPL). This was further supported by the accumulation of secondary metabolites of a phenolic nature that showed an increase of up to sixfold in inhibition capacity of bacterial growth in vitro. The bulk of the antimicrobial activity was found in the acid-hydrolyzed extract containing the phenolics in their aglycone form. High-performance liquid chromatography analysis of phenolic compounds showed a marked change in their profile in the challenged, preelicited plants relative to that in challenged controls. The results suggest that similar to beneficial rhizobacteria, T. asperellum may activate separate metabolic pathways in cucumber that are involved in plant signaling and biosynthesis, eventually leading to the systemic accumulation of phytoalexins.
Scientific Publication
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