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פותח על ידי קלירמאש פתרונות בע"מ -
Expression of the grape dihydroflavonol reductase gene and analysis of its promoter region
Year:
2002
Source of publication :
Journal of Experimental Botany
Authors :
אבן, סילבי
;
.
גולופ, רחל
;
.
Volume :
53
Co-Authors:
Gollop, R., Department of Fruit Tree Breeding, ARO, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Even, S., Department of Fruit Tree Breeding, ARO, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Colova-Tsolova, V., Department of Fruit Tree Breeding, ARO, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Perl, A., Department of Fruit Tree Breeding, ARO, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Facilitators :
From page:
1397
To page:
1409
(
Total pages:
13
)
Abstract:
Dihydroflavonol reductase (DFR) is a key enzyme involved in anthocyanin biosynthesis and proanthocyanidin synthesis in grape. DFR catalyses the reduction of dihydroflavonols to leucoanthocyanidins in the anthocyanin pathway. The DFR products, the leucoanthocyanidins, are substrates for the next step in the anthocyanin pathway and are also the substrates for the proanthocyanidin pathway. In the present study the promoter of the grape dfr gene was cloned. Analysis of the dfr promoter sequence revealed the existence of several putative DNA binding motifs. The dfr promoter was fused to the uidA gene and the control of this fusion and the endogenous dfr gene expression, was studied in transformed plants and in red cell suspension originated from fruits. The dfr promoter-uidA gene fusion was expressed in leaves, roots and stems. Deletions of the dfr promoter influenced the specificity of the expression of the GUS gene fusion in plantlet roots and the level of expression in plants and in the red cell suspension originated from fruits. The deletion analysis of the dfr promoter suggests that a specific sequence located between -725 to -233 might be involved in expression of the dfr gene in fruits. Light, calcium and sucrose induced the dfr gene expression. In the transformed suspension cultures, expression of both the endogenous dfr gene and the dfr promoter-uidA gene fusions was induced by white light. The induction by both light and calcium suggests the possible involvement of a UV receptors signal transduction pathway in the induction of the dfr gene. The induction of the dfr gene and the dfr promoter-uidA gene fusions by light and sucrose indicates a close interaction between sucrose and light signalling pathways.
Note:
Related Files :
biosynthesis
DNA
enzymes
Genetics
light
molecular genetics
Vitis
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23152
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:57
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Scientific Publication
Expression of the grape dihydroflavonol reductase gene and analysis of its promoter region
53
Gollop, R., Department of Fruit Tree Breeding, ARO, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Even, S., Department of Fruit Tree Breeding, ARO, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Colova-Tsolova, V., Department of Fruit Tree Breeding, ARO, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Perl, A., Department of Fruit Tree Breeding, ARO, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Expression of the grape dihydroflavonol reductase gene and analysis of its promoter region
Dihydroflavonol reductase (DFR) is a key enzyme involved in anthocyanin biosynthesis and proanthocyanidin synthesis in grape. DFR catalyses the reduction of dihydroflavonols to leucoanthocyanidins in the anthocyanin pathway. The DFR products, the leucoanthocyanidins, are substrates for the next step in the anthocyanin pathway and are also the substrates for the proanthocyanidin pathway. In the present study the promoter of the grape dfr gene was cloned. Analysis of the dfr promoter sequence revealed the existence of several putative DNA binding motifs. The dfr promoter was fused to the uidA gene and the control of this fusion and the endogenous dfr gene expression, was studied in transformed plants and in red cell suspension originated from fruits. The dfr promoter-uidA gene fusion was expressed in leaves, roots and stems. Deletions of the dfr promoter influenced the specificity of the expression of the GUS gene fusion in plantlet roots and the level of expression in plants and in the red cell suspension originated from fruits. The deletion analysis of the dfr promoter suggests that a specific sequence located between -725 to -233 might be involved in expression of the dfr gene in fruits. Light, calcium and sucrose induced the dfr gene expression. In the transformed suspension cultures, expression of both the endogenous dfr gene and the dfr promoter-uidA gene fusions was induced by white light. The induction by both light and calcium suggests the possible involvement of a UV receptors signal transduction pathway in the induction of the dfr gene. The induction of the dfr gene and the dfr promoter-uidA gene fusions by light and sucrose indicates a close interaction between sucrose and light signalling pathways.
Scientific Publication
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