Annals of Applied Biology
ROSNER, A., Department of Virology, Volcani Center, Bet Dagan, 50250, Israel
STEIN, A., Department of Virology, Volcani Center, Bet Dagan, 50250, Israel
LEVY, S., Department of Virology, Volcani Center, Bet Dagan, 50250, Israel
The RNA genome of bean yellow mosaic virus (BYMV) was detected in gladioli corms by reverse transcription and polymerase chain reaction (PCR). However, a positive signal was not obtained from some infected corms because the virus concentration was below the sensitivity limits of PCR. Virus was detected in these corms by using a modified protocol in which transcription of the PCR products by T7 RNA polymerase yielded an additional 10‐ to 100‐fold signal amplification. The relative concentrations of BYMV in gladioli corms were evaluated using PCR. Virus accumulation in corms was found to be a 100 times lower than in leaves, and be within the range of picogram quantities of viral RNA per gram fresh weight of corm tissue. Copyright © 1992, Wiley Blackwell. All rights reserved
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Transcription amplification of polymerase chain reaction products of bean yellow mosaic virus RNA extracted from gladioli corms
121
ROSNER, A., Department of Virology, Volcani Center, Bet Dagan, 50250, Israel
STEIN, A., Department of Virology, Volcani Center, Bet Dagan, 50250, Israel
LEVY, S., Department of Virology, Volcani Center, Bet Dagan, 50250, Israel
Transcription amplification of polymerase chain reaction products of bean yellow mosaic virus RNA extracted from gladioli corms
The RNA genome of bean yellow mosaic virus (BYMV) was detected in gladioli corms by reverse transcription and polymerase chain reaction (PCR). However, a positive signal was not obtained from some infected corms because the virus concentration was below the sensitivity limits of PCR. Virus was detected in these corms by using a modified protocol in which transcription of the PCR products by T7 RNA polymerase yielded an additional 10‐ to 100‐fold signal amplification. The relative concentrations of BYMV in gladioli corms were evaluated using PCR. Virus accumulation in corms was found to be a 100 times lower than in leaves, and be within the range of picogram quantities of viral RNA per gram fresh weight of corm tissue. Copyright © 1992, Wiley Blackwell. All rights reserved
Scientific Publication