נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Expression of sarcotoxin IA gene via a root-specific tob promoter enhanced host resistance against parasitic weeds in tomato plants
Year:
2006
Source of publication :
Plant Cell Reports
Authors :
אכדרי, גיא
;
.
עלי, ראדי
;
.
פלקחין, דינה
;
.
Volume :
25
Co-Authors:
Radi, A., Agricultural Research Organization (ARO), Department of Weed Research, Newe Yaar Research Center, Ramat Yishai, 30095, Israel
Dina, P., Agricultural Research Organization (ARO), Department of Weed Research, Newe Yaar Research Center, Ramat Yishai, 30095, Israel
Guy, A., Agricultural Research Organization (ARO), Department of Weed Research, Newe Yaar Research Center, Ramat Yishai, 30095, Israel
Facilitators :
From page:
297
To page:
303
(
Total pages:
7
)
Abstract:
We have developed a simple genetic engineering strategy for conferring resistance against parasitic weeds on host plants. Transgenic tomato plants expressing the sarcotoxin IA gene were grown either in polyethylene bags (PE) or in pots inoculated with Orobanche aegyptiaca seeds. The results indicate that transgenic plants exhibited strong inhibition of parasite growth and significantly increased yield as compared with non-transgenic ones. In both PE and pot systems most of the parasite tubercles attached to the transgenic root plants turned necrotic and developed abnormally. Integration and expression of the gene were confirmed by Southern blot, RT-PCR and Western blot analysis. Our results indicate that the insect gene produced in the plant cells was selectively toxic to the parasite and non-toxic to the host plant. © Springer-Verlag 2005.
Note:
Related Files :
gene expression
genetic engineering
Genetics
germination
Orobanche
Plants
seeds
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00299-005-0052-y
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23251
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:57
You may also be interested in
Scientific Publication
Expression of sarcotoxin IA gene via a root-specific tob promoter enhanced host resistance against parasitic weeds in tomato plants
25
Radi, A., Agricultural Research Organization (ARO), Department of Weed Research, Newe Yaar Research Center, Ramat Yishai, 30095, Israel
Dina, P., Agricultural Research Organization (ARO), Department of Weed Research, Newe Yaar Research Center, Ramat Yishai, 30095, Israel
Guy, A., Agricultural Research Organization (ARO), Department of Weed Research, Newe Yaar Research Center, Ramat Yishai, 30095, Israel
Expression of sarcotoxin IA gene via a root-specific tob promoter enhanced host resistance against parasitic weeds in tomato plants
We have developed a simple genetic engineering strategy for conferring resistance against parasitic weeds on host plants. Transgenic tomato plants expressing the sarcotoxin IA gene were grown either in polyethylene bags (PE) or in pots inoculated with Orobanche aegyptiaca seeds. The results indicate that transgenic plants exhibited strong inhibition of parasite growth and significantly increased yield as compared with non-transgenic ones. In both PE and pot systems most of the parasite tubercles attached to the transgenic root plants turned necrotic and developed abnormally. Integration and expression of the gene were confirmed by Southern blot, RT-PCR and Western blot analysis. Our results indicate that the insect gene produced in the plant cells was selectively toxic to the parasite and non-toxic to the host plant. © Springer-Verlag 2005.
Scientific Publication
You may also be interested in