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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Development of an immunoassay and a sol-gel-based immunoaffinity cleanup method for coplanar polychlorinated biphenyls from soil and sediment samples
Year:
2010
Source of publication :
Analytica Chimica Acta
Authors :
אלטשטיין, מרים
;
.
בן-עזיז, אורנה
;
.
ברונשטיין, אליסה
;
.
סקלקה, ניר
;
.
Volume :
675
Co-Authors:
Altstein, M., Department of Entomology, Volcani Center, Bet Dagan 50250, Israel
Aziz, O.B., Department of Entomology, Volcani Center, Bet Dagan 50250, Israel
Skalka, N., Department of Entomology, Volcani Center, Bet Dagan 50250, Israel
Bronshtein, A., Department of Entomology, Volcani Center, Bet Dagan 50250, Israel
Chuang, J.C., Battelle, Columbus, OH 43201-2693, United States
Van Emon, J.M., National Exposure Research Laboratory, U.S. Environmental Protection Agency, Las Vegas, NV 89193-3478, United States
Facilitators :
From page:
138
To page:
147
(
Total pages:
10
)
Abstract:
Two polychlorinated biphenyls (PCB) enzyme linked immunosorbent assays (ELISAs) were developed using goat PCB purified immunoglobulin (IgG) antibodies (Abs). The IgGs exhibited the highest affinity toward PCB-77 (24ngmL-1) with sensitivities in the range of 6-11ngmL-1. The Abs cross-reacted with PCB-126 and the heptachlorodibenzofuran 1,2,3,4,6,7,8-HpCDF but not with PCB-169, PCB-118, Aroclor 1232, 1248, 1260 or the hexachlorodibenzofuran 2,3,4,6,7,8-HxCDF. The IgGs were also used to develop a sol-gel-based immunoaffinity purification (IAP) method for cleanup of PCB-126. Recovery efficiencies depended on the sol-gel formats; a 1:12 format resulted in the highest binding capacity. Net binding capacity ranged from 112 to 257ng, and 90% of the analyte could be eluted with only 2mL of ethanol. The method was also very efficient in purifying PCB-126 from spiked soil and sediment samples from contaminated sites; and eliminating matrix interferences to a degree that enabled analysis of the purified samples by ELISA. The approaches developed in the course of the study form a basis for the development of additional IAP methods for other PCBs, and their implementation in high-throughput screening programs for PCB in food, soil, and other environmental and biological samples. © 2010 Elsevier B.V.
Note:
Related Files :
Animals
Aroclors
enzymes
ethanol
goats
immunoglobulin G
Recovery efficiency
Sedimentology
soil
Soils
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.aca.2010.07.018
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23253
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:57
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Scientific Publication
Development of an immunoassay and a sol-gel-based immunoaffinity cleanup method for coplanar polychlorinated biphenyls from soil and sediment samples
675
Altstein, M., Department of Entomology, Volcani Center, Bet Dagan 50250, Israel
Aziz, O.B., Department of Entomology, Volcani Center, Bet Dagan 50250, Israel
Skalka, N., Department of Entomology, Volcani Center, Bet Dagan 50250, Israel
Bronshtein, A., Department of Entomology, Volcani Center, Bet Dagan 50250, Israel
Chuang, J.C., Battelle, Columbus, OH 43201-2693, United States
Van Emon, J.M., National Exposure Research Laboratory, U.S. Environmental Protection Agency, Las Vegas, NV 89193-3478, United States
Development of an immunoassay and a sol-gel-based immunoaffinity cleanup method for coplanar polychlorinated biphenyls from soil and sediment samples
Two polychlorinated biphenyls (PCB) enzyme linked immunosorbent assays (ELISAs) were developed using goat PCB purified immunoglobulin (IgG) antibodies (Abs). The IgGs exhibited the highest affinity toward PCB-77 (24ngmL-1) with sensitivities in the range of 6-11ngmL-1. The Abs cross-reacted with PCB-126 and the heptachlorodibenzofuran 1,2,3,4,6,7,8-HpCDF but not with PCB-169, PCB-118, Aroclor 1232, 1248, 1260 or the hexachlorodibenzofuran 2,3,4,6,7,8-HxCDF. The IgGs were also used to develop a sol-gel-based immunoaffinity purification (IAP) method for cleanup of PCB-126. Recovery efficiencies depended on the sol-gel formats; a 1:12 format resulted in the highest binding capacity. Net binding capacity ranged from 112 to 257ng, and 90% of the analyte could be eluted with only 2mL of ethanol. The method was also very efficient in purifying PCB-126 from spiked soil and sediment samples from contaminated sites; and eliminating matrix interferences to a degree that enabled analysis of the purified samples by ELISA. The approaches developed in the course of the study form a basis for the development of additional IAP methods for other PCBs, and their implementation in high-throughput screening programs for PCB in food, soil, and other environmental and biological samples. © 2010 Elsevier B.V.
Scientific Publication
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