אסיף מאגר המחקר החקלאי

Multi-functional T-DNA/Ds tomato lines designed for gene cloning and molecular and physical dissection of the tomato genome

Year:

2003

Source of publication :

Authors :

Volume :

51

Co-Authors:

Gidoni, D., Department of Plant Genetics, Institute of Field Crops, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Fuss, E., Institute for Genetics, University of Cologne, Cologne, Germany, Max Planck Inst. Plant Breed. Res., Cologne, Germany, Inst. Entwicklungs-/M., Heinrich-Heine-Universitaet D., Duesseldorf, Germany
Burbidge, A., Dept. of Physiol./Environ. Science, Sutton Bonington Campus, University of Nottingham, Nottingham, United Kingdom
Speckmann, G.-J., Keygene N.V., Agro Business Park 90, P.O. Box 216, NL-6700 AE Wageningen, Netherlands
James, S., Zeneca Plant Science, Jealott's Hill Research Station, Bracknell Berkshire, United Kingdom
Nijkamp, D., Free University of Amsterdam, Department of Genetics, Inst. for Molec./Biol. Sci. (IMBW), Amsterdam, Netherlands
Mett, A., Department of Plant Genetics, Institute of Field Crops, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Feiler, J., Department of Plant Genetics, Institute of Field Crops, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Smoker, M., John Innes Centre, Sainsbury Laboratory, Norwich, United Kingdom
De Vroomen, M.J., Free University of Amsterdam, Department of Genetics, Inst. for Molec./Biol. Sci. (IMBW), Amsterdam, Netherlands
Leader, D., Zeneca Plant Science, Jealott's Hill Research Station, Bracknell Berkshire, United Kingdom, Wheat Improvement Centre, Syngenta, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom
Liharska, T., Free University of Amsterdam, Department of Genetics, Inst. for Molec./Biol. Sci. (IMBW), Amsterdam, Netherlands
Groenendijk, J., Keygene N.V., Agro Business Park 90, P.O. Box 216, NL-6700 AE Wageningen, Netherlands
Coppoolse, E., Free University of Amsterdam, Department of Genetics, Inst. for Molec./Biol. Sci. (IMBW), Amsterdam, Netherlands
Smit, J.J.M., Free University of Amsterdam, Department of Genetics, Inst. for Molec./Biol. Sci. (IMBW), Amsterdam, Netherlands
Levin, I., Department of Plant Genetics, Institute of Field Crops, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
De Both, M., Keygene N.V., Agro Business Park 90, P.O. Box 216, NL-6700 AE Wageningen, Netherlands
Schuch, W., Zeneca Plant Science, Jealott's Hill Research Station, Bracknell Berkshire, United Kingdom, CellFor Inc., 355 Burrard Street, Vancouver, BC V6C 2G8, Canada
Jones, J.D.G., John Innes Centre, Sainsbury Laboratory, Norwich, United Kingdom
Taylor, I.B., Dept. of Physiol./Environ. Science, Sutton Bonington Campus, University of Nottingham, Nottingham, United Kingdom
Theres, K., Institute for Genetics, University of Cologne, Cologne, Germany, Max Planck Inst. Plant Breed. Res., Cologne, Germany, Max Planck Inst. Plant Breed. Res., Carl-von-Linne-Weg 10, D-50829 Cologne, Germany
Van Haaren, M.J.J., Keygene N.V., Agro Business Park 90, P.O. Box 216, NL-6700 AE Wageningen, Netherlands

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Abstract:

In order to make the tomato genome more accessible for molecular analysis and gene cloning, we have produced 405 individual tomato (Lycopersicon esculentum) lines containing a characterized copy of pJasm13, a multifunctional T-DNA/modified Ds transposon element construct. Both the T-DNA and the Ds element in pJasm13 harbor a set of selectable marker genes to monitor excision and reintegration of Ds and additionally, target sequences for rare cutting restriction enzymes (I-PpoI, SfiI, NotI) and for site-specific recombinases (Cre, FLP, R). Blast analysis of flanking genomic sequences of 174 T-DNA inserts revealed homology to transcribed genes in 69 (40%), of which about half are known or putatively identified as genes and ESTs. The map position of 140 individual inserts was determined on the molecular genetic map of tomato. These inserts are distributed over the 12 chromosomes of tomato, allowing targeted and non-targeted transposon tagging, marking of closely linked genes of interest and induction of chromosomal rearrangements including translocations or creation of saturation-deletions or inversions within defined regions linked to the T-DNA insertion site. The different features of pJasm13 were successfully tested in tomato and Arabidopsis thaliana, thus providing a new tool for molecular/genetic dissection studies, including molecular and physical mapping, mutation analysis and cloning strategies in tomato and potentially, in other plants as well.

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