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פותח על ידי קלירמאש פתרונות בע"מ -
Proliferation of bovine undifferentiated mammary epithelial cells in vitro is modulated by G-proteins
Year:
1990
Authors :
פינס, מרק
;
.
שמאי, אבי
;
.
Volume :
69
Co-Authors:
Shamay, A., Department of Biochemistry and Human Nutrition, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, 76100, Israel
Pines, M., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Rehovot, 76100, Israel
Waksman, M., Department of Biochemistry and Human Nutrition, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, 76100, Israel
Gertler, A., Department of Biochemistry and Human Nutrition, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, 76100, Israel
Facilitators :
From page:
217
To page:
226
(
Total pages:
10
)
Abstract:
Several cAMP-elevating agents such as cholera toxin (CT), forskolin and 3-isobutyl-l-methylxanthine (IBMX) exhibited weak mitogenic activity on bovine undifferentiated mammary epithelial cells in three-dimensional collagen culture. CT and IBMX strongly synergized with epidermal growth factor (EGF), insulin-like growth factor I (IGF-I) or both, but not with 10% fetal calf serum (FCS). Permeable cAMP analogs also synergized with IGF-I. Other hormones such as ovine prolactin, bovine growth hormone, estrogen or progesterone were not mitogenic and not synergistic with EGF, IGF-I, CT and FCS. Pertussis toxin (PT) reduced the DNA synthesis in cells cultured in the basal medium and attenuated 40-90% of the mitogenic activity stimulated by 10% FCS. PT inhibition of DNA synthesis was accompanied by ADP-ribosylation of 40 kDa and 41 kDa membrane proteins. The 41 kDa protein cross-reacted with antibodies that recognize the Gi-protein of the adenylate cyclase system, indicating the involvement of the latter in the mitogenic process. The nature of the second protein remains unknown. The present results suggest that the mitogenesis of normal mammary epithelial cells which is stimulated by IGF-I, EGF and other factors found in FCS is mediated through both cAMP-dependent and independent pathways. These pathways include PT-sensitive GTP-binding proteins. © 1990.
Note:
Related Files :
Animal
Blood
cattle
Cyclic AMP
DNA
Female
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0303-7207(90)90015-Z
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23297
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:58
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Scientific Publication
Proliferation of bovine undifferentiated mammary epithelial cells in vitro is modulated by G-proteins
69
Shamay, A., Department of Biochemistry and Human Nutrition, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, 76100, Israel
Pines, M., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Rehovot, 76100, Israel
Waksman, M., Department of Biochemistry and Human Nutrition, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, 76100, Israel
Gertler, A., Department of Biochemistry and Human Nutrition, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, 76100, Israel
Proliferation of bovine undifferentiated mammary epithelial cells in vitro is modulated by G-proteins
Several cAMP-elevating agents such as cholera toxin (CT), forskolin and 3-isobutyl-l-methylxanthine (IBMX) exhibited weak mitogenic activity on bovine undifferentiated mammary epithelial cells in three-dimensional collagen culture. CT and IBMX strongly synergized with epidermal growth factor (EGF), insulin-like growth factor I (IGF-I) or both, but not with 10% fetal calf serum (FCS). Permeable cAMP analogs also synergized with IGF-I. Other hormones such as ovine prolactin, bovine growth hormone, estrogen or progesterone were not mitogenic and not synergistic with EGF, IGF-I, CT and FCS. Pertussis toxin (PT) reduced the DNA synthesis in cells cultured in the basal medium and attenuated 40-90% of the mitogenic activity stimulated by 10% FCS. PT inhibition of DNA synthesis was accompanied by ADP-ribosylation of 40 kDa and 41 kDa membrane proteins. The 41 kDa protein cross-reacted with antibodies that recognize the Gi-protein of the adenylate cyclase system, indicating the involvement of the latter in the mitogenic process. The nature of the second protein remains unknown. The present results suggest that the mitogenesis of normal mammary epithelial cells which is stimulated by IGF-I, EGF and other factors found in FCS is mediated through both cAMP-dependent and independent pathways. These pathways include PT-sensitive GTP-binding proteins. © 1990.
Scientific Publication
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