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פותח על ידי קלירמאש פתרונות בע"מ -
Functional analysis of the Cucumber mosaic virus 2b protein: Pathogenicity and nuclear localization
Year:
2004
Source of publication :
Journal of General Virology
Authors :
גאבה, ויקטור
;
.
גל-און, עמית
;
.
Volume :
85
Co-Authors:
Wang, Y., Department of Virology, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel, Department of Plant Pathology, Iowa State University, Ames, IA 50011-1020, United States
Tzfira, T., Dept. of Biochemistry/Cell Biology, State Univ. New York at Stony Brook, Stony Brook, NY 11794-5215, United States
Gaba, V., Department of Virology, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel
Citovsky, V., Dept. of Biochemistry/Cell Biology, State Univ. New York at Stony Brook, Stony Brook, NY 11794-5215, United States
Palukaitis, P., Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, United Kingdom
Gal-On, A., Department of Virology, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
3135
To page:
3147
(
Total pages:
13
)
Abstract:
The 2b protein encoded by Cucumber mosaic virus (CMV) has been shown to be a silencing suppressor and pathogenicity determinant in solanaceous hosts, but a movement determinant in cucumber. In addition, synergistic interactions between CMV and Zucchini yellow mosaic virus (ZYMV) have been described in several cucurbit species. Here, it was shown that deletion of the 2b gene from CMV prevented extensive systemic movement of the virus in zucchini squash, which could not be complemented by co-infection with ZYMV. Thus, ZYMV expressing a silencing suppressor with a different target could not complement the CMV 2b-specific movement function. Expression of the 2b protein from an attenuated ZYMV vector resulted in a synergistic response, largely restoring infection symptoms of wild-type ZYMV in several cucurbit species. Deletion or alteration of either of two nuclear localization signals (NLSs) did not affect nuclear localization in two assays, but did affect pathogenicity in several cucurbit species, whilst deletion of both NLSs led to loss of nuclear localization. The 2b protein interacted with an Arabidopsis thaliana karyopherin α protein (AtKAPα) in the yeast two-hybrid system, as did each of the two single NLS-deletion mutants. However, 2b protein containing a deletion of both NLSs was unable to interact with AtKAPα. These data suggest that the 2b protein localizes to the nucleus by using the karyopherin α-mediated system, but demonstrate that nuclear localization was insufficient for enhancement of the 2b-mediated pathogenic response in cucurbit hosts. Thus, the sequences corresponding to the two NLSs must have another role leading to pathogenicity enhancement. © 2004 SGM.
Note:
Related Files :
arabidopsis
Cucumis sativus
gene silencing
molecular genetics
עוד תגיות
תוכן קשור
More details
DOI :
10.1099/vir.0.80250-0
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23326
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:58
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Scientific Publication
Functional analysis of the Cucumber mosaic virus 2b protein: Pathogenicity and nuclear localization
85
Wang, Y., Department of Virology, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel, Department of Plant Pathology, Iowa State University, Ames, IA 50011-1020, United States
Tzfira, T., Dept. of Biochemistry/Cell Biology, State Univ. New York at Stony Brook, Stony Brook, NY 11794-5215, United States
Gaba, V., Department of Virology, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel
Citovsky, V., Dept. of Biochemistry/Cell Biology, State Univ. New York at Stony Brook, Stony Brook, NY 11794-5215, United States
Palukaitis, P., Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, United Kingdom
Gal-On, A., Department of Virology, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel
Functional analysis of the Cucumber mosaic virus 2b protein: Pathogenicity and nuclear localization
The 2b protein encoded by Cucumber mosaic virus (CMV) has been shown to be a silencing suppressor and pathogenicity determinant in solanaceous hosts, but a movement determinant in cucumber. In addition, synergistic interactions between CMV and Zucchini yellow mosaic virus (ZYMV) have been described in several cucurbit species. Here, it was shown that deletion of the 2b gene from CMV prevented extensive systemic movement of the virus in zucchini squash, which could not be complemented by co-infection with ZYMV. Thus, ZYMV expressing a silencing suppressor with a different target could not complement the CMV 2b-specific movement function. Expression of the 2b protein from an attenuated ZYMV vector resulted in a synergistic response, largely restoring infection symptoms of wild-type ZYMV in several cucurbit species. Deletion or alteration of either of two nuclear localization signals (NLSs) did not affect nuclear localization in two assays, but did affect pathogenicity in several cucurbit species, whilst deletion of both NLSs led to loss of nuclear localization. The 2b protein interacted with an Arabidopsis thaliana karyopherin α protein (AtKAPα) in the yeast two-hybrid system, as did each of the two single NLS-deletion mutants. However, 2b protein containing a deletion of both NLSs was unable to interact with AtKAPα. These data suggest that the 2b protein localizes to the nucleus by using the karyopherin α-mediated system, but demonstrate that nuclear localization was insufficient for enhancement of the 2b-mediated pathogenic response in cucurbit hosts. Thus, the sequences corresponding to the two NLSs must have another role leading to pathogenicity enhancement. © 2004 SGM.
Scientific Publication
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