Detection of an open reading frame related to the CMS-associated urf-s in fertile Petunia lines and species and in other fertile Solanaceae species

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Detection of an open reading frame related to the CMS-associated urf-s in fertile Petunia lines and species and in other fertile Solanaceae species

Year: 

1993

Source of publication :

Theoretical and Applied Genetics

Authors :

גדעוני, דוד

;

טביב, יונה

;

יצהר, שמאי

;

סופרמן-אבשלום, א'

;

פירון, נורית

.

Volume :

Co-Authors: 

Soferman-Avshalom, O., Department of Plant Genetics, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Yesodi, V., Department of Plant Genetics, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Tabib, Y., Department of Plant Genetics, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Gidoni, D., Department of Plant Genetics, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Izhar, S., Department of Plant Genetics, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Firon, N., Department of Plant Genetics, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel

From page: 

308

To page: 

311

(

Total pages: 

4

)

Link :

Detection of an open reading frame related to the CMS-associated urf-s in fertile Petunia lines and species and in other fertile Solanaceae species

Abstract: 

In Petunia, a mitochondrial (mt) locus, S-Pcf, has been found to be strongly associated with cytoplasmic male sterility (CMS). The S-Pcf locus consists of three open reading frames (ORF) that are co-transcribed. The first ORF, termed Pcf, contains an unidentified reading frame urf-s that has been detected so far only in sterile Petunia lines and sterile somatic hybrids. In the study described here, a urf-s-related sequence was detected in seven different normal fertile Petunia lines and species as well as in additional members of the Solanaceae family by means of the polymerase chain reaction. The urf-s-related sequence identified in the fertile lines was termed orf152. In Petunia the nucleotide sequence of orf152 was found to be identical to the corresponding part of urf-s. However, the genome organization around orf152 was found to be different from that of urf-s. These results indicate that: (1) at least part of the urf-s sequence is present in fertile lines and species of Petunia and in other Solanaceae species; (2) the orf152 sequence of Petunia is not part of the Pcf ORF. The relevance of these findings to a better understanding of the evolution of the S-pcf locus in (S) cytoplasm in Petunia is discussed. © 1993 Springer-Verlag.