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פותח על ידי קלירמאש פתרונות בע"מ -
Accelerated expansion of group IID-like phospholipase A2 genes in Bos taurus
Year:
2006
Source of publication :
genomics (source)
Authors :
גוליק, מרינה
;
.
ולר, יהודה
;
.
כהן-צינדר, מירי
;
.
סרוסי, אייל
;
.
רון, מיכה
;
.
Volume :
87
Co-Authors:
Golik, M., Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Cohen-Zinder, M., Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Loor, J.J., Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States
Drackley, J.K., Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States
Band, M.R., Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States
Lewin, H.A., Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States
Weller, J.I., Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Ron, M., Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Seroussi, E., Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
527
To page:
533
(
Total pages:
7
)
Abstract:
Low-molecular-weight, calcium-dependent phospholipase A2 genes (PLA2s) that belong to the secreted type of PLA2s are clustered within a syntenic group on human 1p35-p36 and mouse 4qD3. We reassembled trace files available from the Whole Genome Sequencing (WGS) Project, obtaining an 86-kb contig with three tandem PLA2G2D duplications in the Hereford strain. We used mate-pair data to monitor the assembly and to exclude chimeric clones, demonstrating that the current WGS data may be assembled even in a highly repetitive region with a coverage exceeding fivefold. The genomic structure indicated that most of the PLA2G2D transcripts are formed by four exons. Two alternative first exons were present in all duplications. In two duplications insertions of satellite DNA in the third intron created a novel exon that gave rise to a two-exon product. Linkage and comparative mapping placed the bovine PLA2G2 locus on BTA2, indicating that it evolved from an ancestral PLA2G2D locus common to human, cattle, and rodents. Bovine PLA2G2D variants were capable of encoding 147-amino-acid polypeptides that consisted of putative signal peptide and metal-binding domains. Cysteine residues were conserved in positions analogous to those forming the seven disulfide bonds characteristic of PLA2G2 genes. Quantitative PCR analysis of bovine PLA2G2D transcripts indicated that their expression levels varied between the dry period and lactation in the mammary gland samples and that their expression was polymorphic in liver tissue. The recent burst of duplication and divergence of the bovine PLA2G2D genes and their polymorphic nature are typical of innate immune response genes. © 2005 Elsevier Inc. All rights reserved.
Note:
Related Files :
Animals
cattle
chromosome mapping
Female
gene expression
immune response gene
lactation
mice
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.ygeno.2005.12.015
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23352
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:58
You may also be interested in
Scientific Publication
Accelerated expansion of group IID-like phospholipase A2 genes in Bos taurus
87
Golik, M., Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Cohen-Zinder, M., Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Loor, J.J., Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States
Drackley, J.K., Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States
Band, M.R., Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States
Lewin, H.A., Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States
Weller, J.I., Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Ron, M., Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Seroussi, E., Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Accelerated expansion of group IID-like phospholipase A2 genes in Bos taurus
Low-molecular-weight, calcium-dependent phospholipase A2 genes (PLA2s) that belong to the secreted type of PLA2s are clustered within a syntenic group on human 1p35-p36 and mouse 4qD3. We reassembled trace files available from the Whole Genome Sequencing (WGS) Project, obtaining an 86-kb contig with three tandem PLA2G2D duplications in the Hereford strain. We used mate-pair data to monitor the assembly and to exclude chimeric clones, demonstrating that the current WGS data may be assembled even in a highly repetitive region with a coverage exceeding fivefold. The genomic structure indicated that most of the PLA2G2D transcripts are formed by four exons. Two alternative first exons were present in all duplications. In two duplications insertions of satellite DNA in the third intron created a novel exon that gave rise to a two-exon product. Linkage and comparative mapping placed the bovine PLA2G2 locus on BTA2, indicating that it evolved from an ancestral PLA2G2D locus common to human, cattle, and rodents. Bovine PLA2G2D variants were capable of encoding 147-amino-acid polypeptides that consisted of putative signal peptide and metal-binding domains. Cysteine residues were conserved in positions analogous to those forming the seven disulfide bonds characteristic of PLA2G2 genes. Quantitative PCR analysis of bovine PLA2G2D transcripts indicated that their expression levels varied between the dry period and lactation in the mammary gland samples and that their expression was polymorphic in liver tissue. The recent burst of duplication and divergence of the bovine PLA2G2D genes and their polymorphic nature are typical of innate immune response genes. © 2005 Elsevier Inc. All rights reserved.
Scientific Publication
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