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פותח על ידי קלירמאש פתרונות בע"מ -
Replication of tomato yellow leaf curl virus (TYLCV) DNA in agroinoculated leaf discs from selected tomato genotypes
Year:
1993
Source of publication :
Plant Molecular Biology
Authors :
גפני, ידידיה
;
.
Volume :
22
Co-Authors:
Czosnek, H., the Otto Warburg Centre for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76 100, Israel
Kheyr-Pour, A., Institut des Sciences Végétales, Centre National de la Recherche Scientifique, Gif-sur-Yvette, 91198, France
Gronenborn, B., Institut des Sciences Végétales, Centre National de la Recherche Scientifique, Gif-sur-Yvette, 91198, France
Remetz, E., the Otto Warburg Centre for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76 100, Israel
Zeidan, M., the Otto Warburg Centre for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76 100, Israel
Altman, A., the Otto Warburg Centre for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76 100, Israel
Rabinowitch, H.D., the Otto Warburg Centre for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76 100, Israel
Vidavsky, S., the Otto Warburg Centre for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76 100, Israel
Kedar, N., the Otto Warburg Centre for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76 100, Israel
Gafni, Y., Institute of Field and Garden Crops, Department of Genetics, Agricultural Research Organization, P.O. Box 6, Bet Dagan, 50250, Israel
Zamir, D., the Otto Warburg Centre for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76 100, Israel
Facilitators :
From page:
995
To page:
1005
(
Total pages:
11
)
Abstract:
The leaf disc agroinoculation system was applied to study tomato yellow leaf curl virus (TYLCV) replication in explants from susceptible and resistant tomato genotypes. This system was also evaluated as a potential selection tool in breeding programmes for TYLCV resistance. Leaf discs were incubated with a head-to-tail dimer of the TYLCV genome cloned into the Ti plasmid of Agrobacterium tumefaciens. In leaf discs from susceptible cultivars (Lycopersicon esculentum) TYLCV single-stranded genomic DNA and its double-stranded DNA forms appeared within 2-5 days after inoculation. Whiteflies (Bemisia tabaci) efficiently transmitted the TYLCV disease to tomato test plants following acquisition feeding on agroinoculated tomato leaf discs. This indicates that infective viral particles have been produced and have reached the phloem cells of the explant where they can be acquired by the insects. Plants regenerated from agroinfected leaf discs of sensitive tomato cultivars exhibited disease symptoms and contained TYLCV DNA concentrations similar to those present in field-infected tomato plants, indicating that TYLCV can move out from the leaf disc into the regenerating plant. Leaf discs from accessions of the wild tomato species immune to whitefly-mediated inoculation, L. chilense LA1969 and L. hirsutum LA1777, did not support TYLCV DNA replication. Leaf discs from plants tolerant to TYLCV issued from breeding programmes behaved like leaf discs from susceptible cultivars. © 1993 Kluwer Academic Publishers.
Note:
Related Files :
DNA
Genetics
immunity
metabolism
Microbiology
Plant Disease
Plant Diseases
tissue culture
vegetables
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF00028972
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23422
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:59
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Scientific Publication
Replication of tomato yellow leaf curl virus (TYLCV) DNA in agroinoculated leaf discs from selected tomato genotypes
22
Czosnek, H., the Otto Warburg Centre for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76 100, Israel
Kheyr-Pour, A., Institut des Sciences Végétales, Centre National de la Recherche Scientifique, Gif-sur-Yvette, 91198, France
Gronenborn, B., Institut des Sciences Végétales, Centre National de la Recherche Scientifique, Gif-sur-Yvette, 91198, France
Remetz, E., the Otto Warburg Centre for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76 100, Israel
Zeidan, M., the Otto Warburg Centre for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76 100, Israel
Altman, A., the Otto Warburg Centre for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76 100, Israel
Rabinowitch, H.D., the Otto Warburg Centre for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76 100, Israel
Vidavsky, S., the Otto Warburg Centre for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76 100, Israel
Kedar, N., the Otto Warburg Centre for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76 100, Israel
Gafni, Y., Institute of Field and Garden Crops, Department of Genetics, Agricultural Research Organization, P.O. Box 6, Bet Dagan, 50250, Israel
Zamir, D., the Otto Warburg Centre for Biotechnology in Agriculture, P.O. Box 12, Rehovot, 76 100, Israel
Replication of tomato yellow leaf curl virus (TYLCV) DNA in agroinoculated leaf discs from selected tomato genotypes
The leaf disc agroinoculation system was applied to study tomato yellow leaf curl virus (TYLCV) replication in explants from susceptible and resistant tomato genotypes. This system was also evaluated as a potential selection tool in breeding programmes for TYLCV resistance. Leaf discs were incubated with a head-to-tail dimer of the TYLCV genome cloned into the Ti plasmid of Agrobacterium tumefaciens. In leaf discs from susceptible cultivars (Lycopersicon esculentum) TYLCV single-stranded genomic DNA and its double-stranded DNA forms appeared within 2-5 days after inoculation. Whiteflies (Bemisia tabaci) efficiently transmitted the TYLCV disease to tomato test plants following acquisition feeding on agroinoculated tomato leaf discs. This indicates that infective viral particles have been produced and have reached the phloem cells of the explant where they can be acquired by the insects. Plants regenerated from agroinfected leaf discs of sensitive tomato cultivars exhibited disease symptoms and contained TYLCV DNA concentrations similar to those present in field-infected tomato plants, indicating that TYLCV can move out from the leaf disc into the regenerating plant. Leaf discs from accessions of the wild tomato species immune to whitefly-mediated inoculation, L. chilense LA1969 and L. hirsutum LA1777, did not support TYLCV DNA replication. Leaf discs from plants tolerant to TYLCV issued from breeding programmes behaved like leaf discs from susceptible cultivars. © 1993 Kluwer Academic Publishers.
Scientific Publication
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