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פותח על ידי קלירמאש פתרונות בע"מ -
A 'golden' SNP in CmOr governs the fruit flesh color of melon (Cucumis melo)
Year:
2015
Source of publication :
Plant Journal
Authors :
באומקולר, פביאן
;
.
בורגר, יוסף
;
.
חיות, נועם
;
.
לוינסון, אפרים
;
.
מאיר, איילה
;
.
סער, עוזי
;
.
פורטנוי, ויטלי
;
.
צורי, גליל
;
.
קציר, נורית
;
.
שפר, ארתור
;
.
תדמור, יעקב
;
.
Volume :
82
Co-Authors:
Tzuri, G., Plant Science Institute, Israeli Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay, Israel
Zhou, X., Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY, United States
Chayut, N., Plant Science Institute, Israeli Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay, Israel, Faculty of Biology, Technion - Israel Institute of Technology, Haifa, Israel
Yuan, H., Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY, United States
Portnoy, V., Plant Science Institute, Israeli Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay, Israel
Meir, A., Plant Science Institute, Israeli Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay, Israel
Sa'Ar, U., Plant Science Institute, Israeli Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay, Israel
Baumkoler, F., Plant Science Institute, Israeli Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay, Israel
Mazourek, M., Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY, United States
Lewinsohn, E., Plant Science Institute, Israeli Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay, Israel
Fei, Z., Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY, United States
Schaffer, A.A., Plant Science Institute, Agricultural Research Organization, Volcani Center, P.O.B. 6, Bet-Dagan, Israel
Li, L., Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY, United States, US Department of Agriculture-Agricultural Research Service, Robert W Holly Center for Agriculture and Health, Cornell University, Ithaca, NY, United States
Burger, J., Plant Science Institute, Israeli Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay, Israel
Katzir, N., Plant Science Institute, Israeli Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay, Israel
Tadmor, Y., Plant Science Institute, Israeli Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay, Israel
Facilitators :
From page:
267
To page:
279
(
Total pages:
13
)
Abstract:
Summary The flesh color of Cucumis melo (melon) is genetically determined, and can be white, light green or orange, with β-carotene being the predominant pigment. We associated carotenoid accumulation in melon fruit flesh with polymorphism within CmOr, a homolog of the cauliflower BoOr gene, and identified CmOr as the previously described gf locus in melon. CmOr was found to co-segregate with fruit flesh color, and presented two haplotypes (alleles) in a broad germplasm collection, one being associated with orange flesh and the second being associated with either white or green flesh. Allelic variation of CmOr does not affect its transcription or protein level. The variation also does not affect its plastid subcellular localization. Among the identified single nucleotide polymorphisms (SNPs) between CmOr alleles in orange versus green/white-flesh fruit, a single SNP causes a change of an evolutionarily highly conserved arginine to histidine in the CmOr protein. Functional analysis of CmOr haplotypes in an Arabidopsis callus system confirmed the ability of the CmOr orange haplotype to induce β-carotene accumulation. Site-directed mutagenesis of the CmOr green/white haplotype to change the CmOR arginine to histidine triggered β-carotene accumulation. The identification of the 'golden' SNP in CmOr, which is responsible for the non-orange and orange melon fruit phenotypes, provides new tools for studying the Or mechanism of action, and suggests genome editing of the Or gene for nutritional biofortification of crops. Significance Statement Our work demonstrates that a change of arginine to histidine in an evolutionarily highly-conserved location in Or proteins, is responsible for b-carotene accumulation in melon fruit and is capable to induce b-carotene accumulation in a heterologous system. This "golden" SNP could lead not only to better understanding of the mechanism underlying Or-induced carotenoid accumulation, but also to new approaches for biofortification of agricultural products with pro-vitamin A via genome-editing technology. © 2015 The Authors The Plant Journal © 2015 John Wiley & Sons Ltd.
Note:
Related Files :
arabidopsis
color
Cucumis melo
Genetics
metabolism
molecular genetics
pigmentation
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/tpj.12814
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23436
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:59
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Scientific Publication
A 'golden' SNP in CmOr governs the fruit flesh color of melon (Cucumis melo)
82
Tzuri, G., Plant Science Institute, Israeli Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay, Israel
Zhou, X., Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY, United States
Chayut, N., Plant Science Institute, Israeli Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay, Israel, Faculty of Biology, Technion - Israel Institute of Technology, Haifa, Israel
Yuan, H., Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY, United States
Portnoy, V., Plant Science Institute, Israeli Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay, Israel
Meir, A., Plant Science Institute, Israeli Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay, Israel
Sa'Ar, U., Plant Science Institute, Israeli Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay, Israel
Baumkoler, F., Plant Science Institute, Israeli Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay, Israel
Mazourek, M., Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY, United States
Lewinsohn, E., Plant Science Institute, Israeli Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay, Israel
Fei, Z., Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY, United States
Schaffer, A.A., Plant Science Institute, Agricultural Research Organization, Volcani Center, P.O.B. 6, Bet-Dagan, Israel
Li, L., Department of Plant Breeding and Genetics, Cornell University, Ithaca, NY, United States, US Department of Agriculture-Agricultural Research Service, Robert W Holly Center for Agriculture and Health, Cornell University, Ithaca, NY, United States
Burger, J., Plant Science Institute, Israeli Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay, Israel
Katzir, N., Plant Science Institute, Israeli Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay, Israel
Tadmor, Y., Plant Science Institute, Israeli Agricultural Research Organization, Newe ya'Ar Research Center, P.O. Box 1021, Ramat Yishay, Israel
A 'golden' SNP in CmOr governs the fruit flesh color of melon (Cucumis melo)
Summary The flesh color of Cucumis melo (melon) is genetically determined, and can be white, light green or orange, with β-carotene being the predominant pigment. We associated carotenoid accumulation in melon fruit flesh with polymorphism within CmOr, a homolog of the cauliflower BoOr gene, and identified CmOr as the previously described gf locus in melon. CmOr was found to co-segregate with fruit flesh color, and presented two haplotypes (alleles) in a broad germplasm collection, one being associated with orange flesh and the second being associated with either white or green flesh. Allelic variation of CmOr does not affect its transcription or protein level. The variation also does not affect its plastid subcellular localization. Among the identified single nucleotide polymorphisms (SNPs) between CmOr alleles in orange versus green/white-flesh fruit, a single SNP causes a change of an evolutionarily highly conserved arginine to histidine in the CmOr protein. Functional analysis of CmOr haplotypes in an Arabidopsis callus system confirmed the ability of the CmOr orange haplotype to induce β-carotene accumulation. Site-directed mutagenesis of the CmOr green/white haplotype to change the CmOR arginine to histidine triggered β-carotene accumulation. The identification of the 'golden' SNP in CmOr, which is responsible for the non-orange and orange melon fruit phenotypes, provides new tools for studying the Or mechanism of action, and suggests genome editing of the Or gene for nutritional biofortification of crops. Significance Statement Our work demonstrates that a change of arginine to histidine in an evolutionarily highly-conserved location in Or proteins, is responsible for b-carotene accumulation in melon fruit and is capable to induce b-carotene accumulation in a heterologous system. This "golden" SNP could lead not only to better understanding of the mechanism underlying Or-induced carotenoid accumulation, but also to new approaches for biofortification of agricultural products with pro-vitamin A via genome-editing technology. © 2015 The Authors The Plant Journal © 2015 John Wiley & Sons Ltd.
Scientific Publication
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