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פותח על ידי קלירמאש פתרונות בע"מ -
Characterization of the early development of specific hypaxial muscles from the ventrolateral myotome
Year:
1999
Source of publication :
Development
Authors :
צינמון, יובל
;
.
Volume :
126
Co-Authors:
Cinnamon, Y., Dept. of Anatomy and Cell Biology, Hebrew Univ.-Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel
Kahane, N., Dept. of Anatomy and Cell Biology, Hebrew Univ.-Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel
Kalcheim, C., Dept. of Anatomy and Cell Biology, Hebrew Univ.-Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel
Facilitators :
From page:
4305
To page:
4315
(
Total pages:
11
)
Abstract:
We have previously found that the myotome is formed by a first wave of pioneer cells generated along the medial epithelial somite and a second wave emanating from the dorsomedial lip (DML), rostral and caudal edges of the dermomyotome. In this study, we have addressed the development and precise fate of the ventrolateral lip (VLL) in non-limb regions of the axis. To this end, fluorescent vital dyes were iontophoretically injected in the center of the VLL and the translocation of labeled cells was followed by confocal microscopy. VLL-derived cells colonized the ventrolateral portion of the myotome. This occurred following an early longitudinal cell translocation along the medial boundary until reaching the rostral or caudal dermomyotome lips from which fibers emerged into the myotome. Thus, the behavior of VLL cells parallels that of their DML counterparts which colonize the opposite, dorsomedial portion of the myotome. To precisely understand the way the myotome expands, we addressed the early generation of hypaxial intercostal muscles. We found that intercostal muscles were formed by VLL-derived fibers that intermingled with fibers emerging from the ventrolateral aspect of both rostral and caudal edges of the dermomyotome. Notably, hypaxial intercostal muscles also contained pioneer myofibers (first wave) showing for the first time that lateral myotome-derived muscles contain a fundamental component of fibers generated in the medial domain of the somite. In addition, we show that during myotome growth and evolution into muscle, second-wave myofibers progressively intercalate between the pioneer fibers, suggesting a constant mode of myotomal expansion in its dorsomedial to ventrolateral extent. This further suggests that specific hypaxial muscles develop following a consistent ventral expansion of a 'compound myotome' into the somatopleure.
Note:
Related Files :
Animals
animal tissue
Dorsomedial lip
Models, Biological
muscle cell
Myogenesis
sclerotome
somite
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23437
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:59
Scientific Publication
Characterization of the early development of specific hypaxial muscles from the ventrolateral myotome
126
Cinnamon, Y., Dept. of Anatomy and Cell Biology, Hebrew Univ.-Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel
Kahane, N., Dept. of Anatomy and Cell Biology, Hebrew Univ.-Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel
Kalcheim, C., Dept. of Anatomy and Cell Biology, Hebrew Univ.-Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel
Characterization of the early development of specific hypaxial muscles from the ventrolateral myotome
We have previously found that the myotome is formed by a first wave of pioneer cells generated along the medial epithelial somite and a second wave emanating from the dorsomedial lip (DML), rostral and caudal edges of the dermomyotome. In this study, we have addressed the development and precise fate of the ventrolateral lip (VLL) in non-limb regions of the axis. To this end, fluorescent vital dyes were iontophoretically injected in the center of the VLL and the translocation of labeled cells was followed by confocal microscopy. VLL-derived cells colonized the ventrolateral portion of the myotome. This occurred following an early longitudinal cell translocation along the medial boundary until reaching the rostral or caudal dermomyotome lips from which fibers emerged into the myotome. Thus, the behavior of VLL cells parallels that of their DML counterparts which colonize the opposite, dorsomedial portion of the myotome. To precisely understand the way the myotome expands, we addressed the early generation of hypaxial intercostal muscles. We found that intercostal muscles were formed by VLL-derived fibers that intermingled with fibers emerging from the ventrolateral aspect of both rostral and caudal edges of the dermomyotome. Notably, hypaxial intercostal muscles also contained pioneer myofibers (first wave) showing for the first time that lateral myotome-derived muscles contain a fundamental component of fibers generated in the medial domain of the somite. In addition, we show that during myotome growth and evolution into muscle, second-wave myofibers progressively intercalate between the pioneer fibers, suggesting a constant mode of myotomal expansion in its dorsomedial to ventrolateral extent. This further suggests that specific hypaxial muscles develop following a consistent ventral expansion of a 'compound myotome' into the somatopleure.
Scientific Publication
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