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A recombination hotspot delimits a wild-species quantitative trait locus for tomato sugar content to 484 bp within an invertase gene
Year:
2000
Authors :
פרידמן, איל
;
.
Volume :
97
Co-Authors:
Fridman, E., Otto Warburg Ctr. for Biotechnology, Fac. Agric., Food and Environ. Sci., Hebrew University of Jerusalem, Rehovot 76100, Israel
Pleban, T., Otto Warburg Ctr. for Biotechnology, Fac. Agric., Food and Environ. Sci., Hebrew University of Jerusalem, Rehovot 76100, Israel
Zamir, D., Otto Warburg Ctr. for Biotechnology, Fac. Agric., Food and Environ. Sci., Hebrew University of Jerusalem, Rehovot 76100, Israel
Facilitators :
From page:
4718
To page:
4723
(
Total pages:
6
)
Abstract:
In nature, genetic variation usually takes the form of a continuous phenotypic range rather than discrete classes. The genetic variation underlying quantitative traits results from the segregation of numerous interacting quantitative trait loci (QTLs), whose expression is modified by the environment. To uncover the molecular basis of this variation, we characterized a QTL (Brix9-2-5) derived from the green-fruited tomato species Lycopersicon pennellii. The wild-species allele increased glucose and fructose contents in cultivated tomato fruits in various genetic backgrounds and environments. Using nearly isogenic lines for the QTL, high-resolution mapping analysis delimited Brix9-2-5 to a single nucleotide polymorphism- defined recombination hotspot of 484 bp spanning an exon and intron of a fruit-specific apoplastic invertase. We suggest that the differences between the Brix9-2-5 alleles of the two species are associated with a polymorphic intronic element that modulates sink strength of tomato fruits. Our results demonstrate a link between naturally occurring DNA variation and a Mendelian determinant of a complex phenotype for a yield-associated trait.
Note:
Related Files :
Base Sequence
gene expression
gene mapping
genetic recombination
Glycoside Hydrolases
Heterozygote
Molecular Sequence Data
phenotype
עוד תגיות
תוכן קשור
More details
DOI :
10.1073/pnas.97.9.4718
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23464
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:59
Scientific Publication
A recombination hotspot delimits a wild-species quantitative trait locus for tomato sugar content to 484 bp within an invertase gene
97
Fridman, E., Otto Warburg Ctr. for Biotechnology, Fac. Agric., Food and Environ. Sci., Hebrew University of Jerusalem, Rehovot 76100, Israel
Pleban, T., Otto Warburg Ctr. for Biotechnology, Fac. Agric., Food and Environ. Sci., Hebrew University of Jerusalem, Rehovot 76100, Israel
Zamir, D., Otto Warburg Ctr. for Biotechnology, Fac. Agric., Food and Environ. Sci., Hebrew University of Jerusalem, Rehovot 76100, Israel
A recombination hotspot delimits a wild-species quantitative trait locus for tomato sugar content to 484 bp within an invertase gene
In nature, genetic variation usually takes the form of a continuous phenotypic range rather than discrete classes. The genetic variation underlying quantitative traits results from the segregation of numerous interacting quantitative trait loci (QTLs), whose expression is modified by the environment. To uncover the molecular basis of this variation, we characterized a QTL (Brix9-2-5) derived from the green-fruited tomato species Lycopersicon pennellii. The wild-species allele increased glucose and fructose contents in cultivated tomato fruits in various genetic backgrounds and environments. Using nearly isogenic lines for the QTL, high-resolution mapping analysis delimited Brix9-2-5 to a single nucleotide polymorphism- defined recombination hotspot of 484 bp spanning an exon and intron of a fruit-specific apoplastic invertase. We suggest that the differences between the Brix9-2-5 alleles of the two species are associated with a polymorphic intronic element that modulates sink strength of tomato fruits. Our results demonstrate a link between naturally occurring DNA variation and a Mendelian determinant of a complex phenotype for a yield-associated trait.
Scientific Publication
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