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פותח על ידי קלירמאש פתרונות בע"מ -
Sugar-dependent gibberellin-induced chalcone synthase gene expression in Petunia corollas
Year:
1997
Authors :
בנו-מועלם, דלילה
;
.
Volume :
113
Co-Authors:
Moalem-Beno, D., Kennedy-Leigh Ctr. for Hort. Res., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel, Inst. Technol. Storage Agric. Prod., Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Tamari, G., Kennedy-Leigh Ctr. for Hort. Res., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Leitner-Dagan, Y., Kennedy-Leigh Ctr. for Hort. Res., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Borochov, A., Kennedy-Leigh Ctr. for Hort. Res., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Weiss, D., Kennedy-Leigh Ctr. for Hort. Res., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Facilitators :
From page:
419
To page:
424
(
Total pages:
6
)
Abstract:
The induction of anthocyanin synthesis and anthocyanin biosynthetic gene expression in detached petunia (Petunia hybrida) corollas by gibberellic acid (GA3) requires sucrose. Neither sucrose nor GA3 alone can induce these processes. We found that GA3 enhances sucrose uptake by 20 to 30%, and we tested whether this is the mechanism by which the hormone induces gene expression. Changing the intracellular level of sucrose with the inhibitors p-chloromercuribenzenesulfonic acid and vanadate did not inhibit the induction of chalcone synthase gene (chs) expression by GA3. Growing detached corollas in various sucrose concentrations did not affect the induction of the gene but did affect its level of expression and the level of anthocyanin accumulated. Only metabolic sugars promoted GA3-induced anthocyanin accumulation. Mannitol and sorbitol had no effect and 3-O-methylglucose only slightly promoted chs expression and anthocyanin accumulation. Our results do not support the suggestion that sugars act as specific signals in the activation of anthocyanin biosynthetic gene expression during petunia corolla development. We suggest that sugars are essential as general sources of carbohydrates for carbon metabolism, upon which the induction of pigmentation is dependent.
Note:
Related Files :
Petunia corollas
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23471
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:59
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Scientific Publication
Sugar-dependent gibberellin-induced chalcone synthase gene expression in Petunia corollas
113
Moalem-Beno, D., Kennedy-Leigh Ctr. for Hort. Res., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel, Inst. Technol. Storage Agric. Prod., Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Tamari, G., Kennedy-Leigh Ctr. for Hort. Res., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Leitner-Dagan, Y., Kennedy-Leigh Ctr. for Hort. Res., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Borochov, A., Kennedy-Leigh Ctr. for Hort. Res., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Weiss, D., Kennedy-Leigh Ctr. for Hort. Res., Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Sugar-dependent gibberellin-induced chalcone synthase gene expression in Petunia corollas
The induction of anthocyanin synthesis and anthocyanin biosynthetic gene expression in detached petunia (Petunia hybrida) corollas by gibberellic acid (GA3) requires sucrose. Neither sucrose nor GA3 alone can induce these processes. We found that GA3 enhances sucrose uptake by 20 to 30%, and we tested whether this is the mechanism by which the hormone induces gene expression. Changing the intracellular level of sucrose with the inhibitors p-chloromercuribenzenesulfonic acid and vanadate did not inhibit the induction of chalcone synthase gene (chs) expression by GA3. Growing detached corollas in various sucrose concentrations did not affect the induction of the gene but did affect its level of expression and the level of anthocyanin accumulated. Only metabolic sugars promoted GA3-induced anthocyanin accumulation. Mannitol and sorbitol had no effect and 3-O-methylglucose only slightly promoted chs expression and anthocyanin accumulation. Our results do not support the suggestion that sugars act as specific signals in the activation of anthocyanin biosynthetic gene expression during petunia corolla development. We suggest that sugars are essential as general sources of carbohydrates for carbon metabolism, upon which the induction of pigmentation is dependent.
Scientific Publication
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