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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Structural characterization and localization analysis of the root-knot nematode Meloidogyne javanica fatty acid and retinol binding protein (Mj-FAR-1)
Year:
2015
Authors :
איברקלייד, יונית
;
.
בלאוסוב, אדוארד
;
.
הורוביץ, סיגל בראון
;
.
צ'ינאפאנדי, ב'
;
.
Volume :
143
Co-Authors:
Iberkleid, I., Department of Entomology, Nematology and Chemistry Units, the Volcani Center, Agricultural Research Organization (ARO), P.O. Box 6, Bet-Dagan, Israel, Department of Plant Pathology and Microbiology, the Robert H. Smith Faculty of Agriculture, Food & Environment, the Hebrew University of Jerusalem, Rehovot, Israel
Yeheskel, A., The Bioinformatics Unit, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
Belausov, E., Department of Plant Sciences, the Volcani Center, Agricultural Research Organization (ARO), Bet Dagan, Israel
Chinnapandi, B., Department of Entomology, Nematology and Chemistry Units, the Volcani Center, Agricultural Research Organization (ARO), P.O. Box 6, Bet-Dagan, Israel
Fitoussi, N., Department of Entomology, Nematology and Chemistry Units, the Volcani Center, Agricultural Research Organization (ARO), P.O. Box 6, Bet-Dagan, Israel, Department of Plant Pathology and Microbiology, the Robert H. Smith Faculty of Agriculture, Food & Environment, the Hebrew University of Jerusalem, Rehovot, Israel
Miyara, S.B., Department of Entomology, Nematology and Chemistry Units, the Volcani Center, Agricultural Research Organization (ARO), P.O. Box 6, Bet-Dagan, Israel
Facilitators :
From page:
133
To page:
149
(
Total pages:
17
)
Abstract:
Plant-parasitic nematodes are extremely destructive pathogens with a cosmopolitan distribution and a host range that affects most crops. They are characterized by distinct parasitic lifestyles, e.g., as sedentary or migratory endo- or ectoparasites, resulting in high losses in yield and revenue. Possessing limited lipid metabolism, they produce one or two structurally unique classes of small α-helix-rich fatty acid and retinol binding (FAR) proteins that have no counterpart in other organisms. We investigated the sequence and structural characteristics of the FAR protein of the root-knot nematode Meloidogyne javanica (Mj-FAR-1) in comparison to other studied FAR proteins. Protein sequence analyses enabled phylogenetic clustering according to trophic groups and lifestyles. Bioinformatics analysis of the FAR protein sequences revealed ten likely core amino acids representing the trophic-group clustering. Clear modifications of four of these amino acids from less reactive (nonpolar, with aliphatic R group) to more reactive (positively or negatively charged R groups, or uncharged polar R groups) might distinguish free-living from parasitic nematode species. Structural predictions of the mature Mj-FAR-1 protein and its ligand-binding pockets, suggest that adaptation toward parasitism is associated with increased reactivity of the second pocket residues, as well as those on the protein surface. Subcellular localization of Mj-FAR-1 with or without its signal peptide was determined by Agrobacterium infiltration of N-terminal mCherry-tagged protein into Nicotiana benthamiana leaves. Intact Mj-FAR-1 with its signal peptide was predominantly localized along the plasma membrane surrounding plant cells while removing the signal peptide resulted in additional localization within the cell nucleus. The nuclear localization agreed with in-silico analysis of the Mj-FAR-1 sequence and sheds new light on its function in manipulating the plant response. Our study provides the first basic structural information and subcellular localization of the plant-parasitic Mj-FAR-1 protein. © 2015, Koninklijke Nederlandse Planteziektenkundige Vereniging.
Note:
Related Files :
Agrobacterium
bioinformatics
Meloidogyne
metabolism
Nematoda
phylogenetics
Plant-parasitic nematode
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s10658-015-0671-2
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23521
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:00
You may also be interested in
Scientific Publication
Structural characterization and localization analysis of the root-knot nematode Meloidogyne javanica fatty acid and retinol binding protein (Mj-FAR-1)
143
Iberkleid, I., Department of Entomology, Nematology and Chemistry Units, the Volcani Center, Agricultural Research Organization (ARO), P.O. Box 6, Bet-Dagan, Israel, Department of Plant Pathology and Microbiology, the Robert H. Smith Faculty of Agriculture, Food & Environment, the Hebrew University of Jerusalem, Rehovot, Israel
Yeheskel, A., The Bioinformatics Unit, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
Belausov, E., Department of Plant Sciences, the Volcani Center, Agricultural Research Organization (ARO), Bet Dagan, Israel
Chinnapandi, B., Department of Entomology, Nematology and Chemistry Units, the Volcani Center, Agricultural Research Organization (ARO), P.O. Box 6, Bet-Dagan, Israel
Fitoussi, N., Department of Entomology, Nematology and Chemistry Units, the Volcani Center, Agricultural Research Organization (ARO), P.O. Box 6, Bet-Dagan, Israel, Department of Plant Pathology and Microbiology, the Robert H. Smith Faculty of Agriculture, Food & Environment, the Hebrew University of Jerusalem, Rehovot, Israel
Miyara, S.B., Department of Entomology, Nematology and Chemistry Units, the Volcani Center, Agricultural Research Organization (ARO), P.O. Box 6, Bet-Dagan, Israel
Structural characterization and localization analysis of the root-knot nematode Meloidogyne javanica fatty acid and retinol binding protein (Mj-FAR-1)
Plant-parasitic nematodes are extremely destructive pathogens with a cosmopolitan distribution and a host range that affects most crops. They are characterized by distinct parasitic lifestyles, e.g., as sedentary or migratory endo- or ectoparasites, resulting in high losses in yield and revenue. Possessing limited lipid metabolism, they produce one or two structurally unique classes of small α-helix-rich fatty acid and retinol binding (FAR) proteins that have no counterpart in other organisms. We investigated the sequence and structural characteristics of the FAR protein of the root-knot nematode Meloidogyne javanica (Mj-FAR-1) in comparison to other studied FAR proteins. Protein sequence analyses enabled phylogenetic clustering according to trophic groups and lifestyles. Bioinformatics analysis of the FAR protein sequences revealed ten likely core amino acids representing the trophic-group clustering. Clear modifications of four of these amino acids from less reactive (nonpolar, with aliphatic R group) to more reactive (positively or negatively charged R groups, or uncharged polar R groups) might distinguish free-living from parasitic nematode species. Structural predictions of the mature Mj-FAR-1 protein and its ligand-binding pockets, suggest that adaptation toward parasitism is associated with increased reactivity of the second pocket residues, as well as those on the protein surface. Subcellular localization of Mj-FAR-1 with or without its signal peptide was determined by Agrobacterium infiltration of N-terminal mCherry-tagged protein into Nicotiana benthamiana leaves. Intact Mj-FAR-1 with its signal peptide was predominantly localized along the plasma membrane surrounding plant cells while removing the signal peptide resulted in additional localization within the cell nucleus. The nuclear localization agreed with in-silico analysis of the Mj-FAR-1 sequence and sheds new light on its function in manipulating the plant response. Our study provides the first basic structural information and subcellular localization of the plant-parasitic Mj-FAR-1 protein. © 2015, Koninklijke Nederlandse Planteziektenkundige Vereniging.
Scientific Publication
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