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פותח על ידי קלירמאש פתרונות בע"מ -
Increase in reactive oxygen species (ROS) and in senescence-associated gene transcript (SAG) levels during dark-induced senescence of Pelargonium cuttings, and the effect of gibberellic acid
Year:
2006
Source of publication :
Plant Science
Authors :
פרידמן, חיה
;
.
רוזנווסר, שילה
;
.
Volume :
170
Co-Authors:
Rosenvasser, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Mayak, S., Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Friedman, H., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
873
To page:
879
(
Total pages:
7
)
Abstract:
Dark-induced senescence in the leaves of Pelargonium cuttings was manifested in chlorophyll breakdown, and an increase in reactive oxygen species (ROS) levels followed by a subsequent induction of two senescence-associated gene (SAG) transcripts: senescence-related transcription factor PeWRKY6-1 and cystein protease homolog PeSAG12-1. Glutathione applied at the onset of ROS increase, reduced ROS accumulation and prevented the increase in PeSAG12-1 expression. These results suggest that in darkness PeSAG12-1 and maybe PeWRKY6-1 are induced by increases in ROS levels. Since PeWRKY6-1 expression increased concomitantly with that of PeSAG12-1, it most likely does not function as a senescence inducer. Application of gibberellic acid (GA3) to Pelargonium cuttings before the dark treatment prevented chlorophyll breakdown, ROS increase and PeWRKY6-1 and PeSAG12-1 accumulation. GA3 also decreased ROS levels when it was applied during the dark period at the onset of ROS accumulation, but not when applied after high ROS levels were evident. The pattern of GA3 suppression of ROS levels positively correlated with its inhibitory effect on chlorophyll breakdown. However, GA3 application after ROS accumulation inhibited PeWRKY6-1 and PeSAG12-1 gene expressions, despite high ROS levels in the tissue. Taken together, our results suggest that GA3 acts to inhibit leaf senescence of Pelargonium, probably not only by reducing ROS levels, but also by interfering with senescence regulation, through an as yet unknown mechanism.
Note:
Related Files :
Acids
chlorophyll
genetic engineering
microorganisms
Pelargonium
Pelargonium cuttings
reactive oxygen species
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.plantsci.2005.12.010
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23524
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:00
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Scientific Publication
Increase in reactive oxygen species (ROS) and in senescence-associated gene transcript (SAG) levels during dark-induced senescence of Pelargonium cuttings, and the effect of gibberellic acid
170
Rosenvasser, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Mayak, S., Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Friedman, H., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Increase in reactive oxygen species (ROS) and in senescence-associated gene transcript (SAG) levels during dark-induced senescence of Pelargonium cuttings, and the effect of gibberellic acid
Dark-induced senescence in the leaves of Pelargonium cuttings was manifested in chlorophyll breakdown, and an increase in reactive oxygen species (ROS) levels followed by a subsequent induction of two senescence-associated gene (SAG) transcripts: senescence-related transcription factor PeWRKY6-1 and cystein protease homolog PeSAG12-1. Glutathione applied at the onset of ROS increase, reduced ROS accumulation and prevented the increase in PeSAG12-1 expression. These results suggest that in darkness PeSAG12-1 and maybe PeWRKY6-1 are induced by increases in ROS levels. Since PeWRKY6-1 expression increased concomitantly with that of PeSAG12-1, it most likely does not function as a senescence inducer. Application of gibberellic acid (GA3) to Pelargonium cuttings before the dark treatment prevented chlorophyll breakdown, ROS increase and PeWRKY6-1 and PeSAG12-1 accumulation. GA3 also decreased ROS levels when it was applied during the dark period at the onset of ROS accumulation, but not when applied after high ROS levels were evident. The pattern of GA3 suppression of ROS levels positively correlated with its inhibitory effect on chlorophyll breakdown. However, GA3 application after ROS accumulation inhibited PeWRKY6-1 and PeSAG12-1 gene expressions, despite high ROS levels in the tissue. Taken together, our results suggest that GA3 acts to inhibit leaf senescence of Pelargonium, probably not only by reducing ROS levels, but also by interfering with senescence regulation, through an as yet unknown mechanism.
Scientific Publication
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