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פותח על ידי קלירמאש פתרונות בע"מ -
Generation and exploration of a dense genetic map in a region of a QTL affecting corpora lutea in a Meishan x Yorkshire cross
Year:
2001
Source of publication :
Mammalian Genome
Authors :
ולר, יהודה
;
.
Volume :
12
Co-Authors:
Braunschweig, M.H., Food Animal Biotechnology Center, Department of Veterinary PathoBiology, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, United States
Paszek, A.A., Food Animal Biotechnology Center, Department of Veterinary PathoBiology, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, United States
Weller, J.I., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Da, Y., Food Animal Biotechnology Center, Department of Animal Science, University of Minnesota, St. Paul, MN 55108, United States
Hawken, R.J., Food Animal Biotechnology Center, Department of Veterinary PathoBiology, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, United States
Wheeler, M.B., Department of Animal Science, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States
Schook, L.B., Food Animal Biotechnology Center, Department of Veterinary PathoBiology, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, United States
Alexander, L.J., Food Animal Biotechnology Center, Department of Veterinary PathoBiology, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, United States
Facilitators :
From page:
719
To page:
723
(
Total pages:
5
)
Abstract:
Previously genomic scans revealed quantitative trait loci (QTL) on porcine Chromosome 8 (SSC8) as significantly affecting the number of corpora lutea (CL) in swine. In one study, statistical evidence for the putative QTL was found in the chromosomal region defined by the microsatellites (MS) SW205, SW444, SW206, and SW29. A Yeast Artificial Chromosome library was screened by using the corresponding primers for clones containing these MS by PCR. From five positive YAC clones, 10 additional MS were isolated and mapped to SSC8 with the INRA-University of Minnesota porcine Radiation Hybrid (IMpRH) panel. The genetic map position of the QTL has been refined by addition of these 10 markers. The QTL evaluation included pedigrees of F2-intercross Meishan x Yorkshire design, with phenotypic data of 108 F2 female offspring and genotypic data for 29 MS markers on SSC8. The analysis was performed by using the least squares regression method. The calculated QTL effect for CL obtained by the multilocus least squares method showed a maximum test statistic (F value = 13.98) at position 99 cM between three MS derived from YACs containing SW205 and SW1843 spanning an interval of 7.1 cM. The point-wise (nominal) P-value was 5.21 × 10-6 corresponding to a genome-wide P-value of 0.009. The additive QTL effect explained 17.4% of the phenotypic variance.
Note:
Related Files :
animal experiment
Animals
chromosome mapping
corpus luteum
Female
gene mapping
Mammalia
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s003350020040
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23572
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:00
Scientific Publication
Generation and exploration of a dense genetic map in a region of a QTL affecting corpora lutea in a Meishan x Yorkshire cross
12
Braunschweig, M.H., Food Animal Biotechnology Center, Department of Veterinary PathoBiology, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, United States
Paszek, A.A., Food Animal Biotechnology Center, Department of Veterinary PathoBiology, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, United States
Weller, J.I., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Da, Y., Food Animal Biotechnology Center, Department of Animal Science, University of Minnesota, St. Paul, MN 55108, United States
Hawken, R.J., Food Animal Biotechnology Center, Department of Veterinary PathoBiology, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, United States
Wheeler, M.B., Department of Animal Science, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States
Schook, L.B., Food Animal Biotechnology Center, Department of Veterinary PathoBiology, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, United States
Alexander, L.J., Food Animal Biotechnology Center, Department of Veterinary PathoBiology, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, United States
Generation and exploration of a dense genetic map in a region of a QTL affecting corpora lutea in a Meishan x Yorkshire cross
Previously genomic scans revealed quantitative trait loci (QTL) on porcine Chromosome 8 (SSC8) as significantly affecting the number of corpora lutea (CL) in swine. In one study, statistical evidence for the putative QTL was found in the chromosomal region defined by the microsatellites (MS) SW205, SW444, SW206, and SW29. A Yeast Artificial Chromosome library was screened by using the corresponding primers for clones containing these MS by PCR. From five positive YAC clones, 10 additional MS were isolated and mapped to SSC8 with the INRA-University of Minnesota porcine Radiation Hybrid (IMpRH) panel. The genetic map position of the QTL has been refined by addition of these 10 markers. The QTL evaluation included pedigrees of F2-intercross Meishan x Yorkshire design, with phenotypic data of 108 F2 female offspring and genotypic data for 29 MS markers on SSC8. The analysis was performed by using the least squares regression method. The calculated QTL effect for CL obtained by the multilocus least squares method showed a maximum test statistic (F value = 13.98) at position 99 cM between three MS derived from YACs containing SW205 and SW1843 spanning an interval of 7.1 cM. The point-wise (nominal) P-value was 5.21 × 10-6 corresponding to a genome-wide P-value of 0.009. The additive QTL effect explained 17.4% of the phenotypic variance.
Scientific Publication
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