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Acidification by gluconic acid of mango fruit tissue during colonization via stem end infection by Phomopsis mangiferae
Year:
2010
Source of publication :
Postharvest Biology and Technology
Authors :
אלקן, נעם
;
.
דוידזון, מעין
;
.
פרוסקי, דב
;
.
קובילר, אילנה
;
.
Volume :
55
Co-Authors:
Davidzon, M., Department of Postharvest Science of Fresh Fruit, The Volcani Center, Agricultural Research Organization, Bet Dagan, 50250, Israel
Alkan, N., Department of Postharvest Science of Fresh Fruit, The Volcani Center, Agricultural Research Organization, Bet Dagan, 50250, Israel
Kobiler, I., Department of Postharvest Science of Fresh Fruit, The Volcani Center, Agricultural Research Organization, Bet Dagan, 50250, Israel
Prusky, D., Department of Postharvest Science of Fresh Fruit, The Volcani Center, Agricultural Research Organization, Bet Dagan, 50250, Israel
Facilitators :
From page:
71
To page:
77
(
Total pages:
7
)
Abstract:
Colonization of mango and other deciduous and tropical fruit by Phomopsis mangiferae was accompanied by local acidification of the host tissue. The fungus acidified the host tissue in mango and grape from pH 5.1 and 4.1, respectively, to 3.8 and 2.5. Analysis of the acidification process in colonized fruit showed that gluconic acid was the main organic acid accumulated at the infection site and in the lesion tissue. In liquid culture conditions the relative induction of transcripts of pmgox1, encoding for glucose oxidase (GOX) was 8-12 times greater at pH 7.0 and 8.0 than at pH 4.0. In infected fruit the detection of high levels of transcripts of pmgox1 and the accumulation of gluconic acid and H2O2 in the decayed tissue, suggested that glucose oxidase contributed to the acidification of the tissue. At the same time, transcripts encoding the endopolygalacturonase gene, pmpg1 accumulated greatly under acidic culture conditions, signifying the importance of the acidification in enhancing the pathogenicity of P. mangiferae. Our results indicate that ambient pH is a regulatory cue for processes linked to pathogenicity of postharvest pathogens, and that specific genes are expressed as a result of the environmental pH created by the pathogen. © 2009 Elsevier B.V. All rights reserved.
Note:
Related Files :
fungi
Host acidification
Mango diseases
Phomopsis
Stem end rots
Storage diseases
Vitaceae
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.postharvbio.2009.08.009
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23641
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:01
Scientific Publication
Acidification by gluconic acid of mango fruit tissue during colonization via stem end infection by Phomopsis mangiferae
55
Davidzon, M., Department of Postharvest Science of Fresh Fruit, The Volcani Center, Agricultural Research Organization, Bet Dagan, 50250, Israel
Alkan, N., Department of Postharvest Science of Fresh Fruit, The Volcani Center, Agricultural Research Organization, Bet Dagan, 50250, Israel
Kobiler, I., Department of Postharvest Science of Fresh Fruit, The Volcani Center, Agricultural Research Organization, Bet Dagan, 50250, Israel
Prusky, D., Department of Postharvest Science of Fresh Fruit, The Volcani Center, Agricultural Research Organization, Bet Dagan, 50250, Israel
Acidification by gluconic acid of mango fruit tissue during colonization via stem end infection by Phomopsis mangiferae
Colonization of mango and other deciduous and tropical fruit by Phomopsis mangiferae was accompanied by local acidification of the host tissue. The fungus acidified the host tissue in mango and grape from pH 5.1 and 4.1, respectively, to 3.8 and 2.5. Analysis of the acidification process in colonized fruit showed that gluconic acid was the main organic acid accumulated at the infection site and in the lesion tissue. In liquid culture conditions the relative induction of transcripts of pmgox1, encoding for glucose oxidase (GOX) was 8-12 times greater at pH 7.0 and 8.0 than at pH 4.0. In infected fruit the detection of high levels of transcripts of pmgox1 and the accumulation of gluconic acid and H2O2 in the decayed tissue, suggested that glucose oxidase contributed to the acidification of the tissue. At the same time, transcripts encoding the endopolygalacturonase gene, pmpg1 accumulated greatly under acidic culture conditions, signifying the importance of the acidification in enhancing the pathogenicity of P. mangiferae. Our results indicate that ambient pH is a regulatory cue for processes linked to pathogenicity of postharvest pathogens, and that specific genes are expressed as a result of the environmental pH created by the pathogen. © 2009 Elsevier B.V. All rights reserved.
Scientific Publication
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